Phenotypes associated with this allele

• Allele Symbol: Nlrc4^tm1Nnz
• Allele Name: targeted mutation 1, Gabriel Nunez
• Allele ID: MGI:3664623
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nlrc4^tm1Nnz/Nlrc4^tm1Nnz	involves: 129 * C57BL/6J	MGI:3706937

Genotype
MGI:3706937

hm1

• Allelic Composition: Nlrc4^tm1Nnz/Nlrc4^tm1Nnz
• Genetic Background: involves: 129 * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal macrophage physiology ( J:112671 , J:117251 )

• treatment of macrophages with purified flagellin in the presence or absence of DOTAP which mediates protein delivery into the cytosol does not induce activation of Casp1 in mutants, but does treatment with flagellin and DOTAP induces activation in Tlr5-deficient cells or wild-type macrophages (J:112671)

• in response to Legionella activation, activation of Casp1 which suppresses Legionella activation, does not occur in deficient macrophages (J:117251)

increased susceptibility to bacterial infection ( J:117251 )

• Legionella counts increase 150-fold over a 72 hour period in infected bone marrow-derived macrophages from null mice, whereas macrophages from Tlr4-, Nod1-, or Nlrp3-deficient mice do not support bacterial replication (multiplicity of infection 0.5)

• infected peritoneal macrophages support bacterial growth ~1000-fold

• increases compared to other mutants and controls is not due to differences in uptake of bacteria by macrophages, but permissivity of replication within macrophages

hematopoietic system

abnormal macrophage physiology ( J:112671 , J:117251 )

• treatment of macrophages with purified flagellin in the presence or absence of DOTAP which mediates protein delivery into the cytosol does not induce activation of Casp1 in mutants, but does treatment with flagellin and DOTAP induces activation in Tlr5-deficient cells or wild-type macrophages (J:112671)

• in response to Legionella activation, activation of Casp1 which suppresses Legionella activation, does not occur in deficient macrophages (J:117251)