All Phenotypes Hspa5<tm1.1Alee> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hspa5^tm1.1Alee/Hspa5^tm1.1Alee	involves: 129 * C57BL/6 * FVB/N	MGI:3712814
cn2	Hspa5^tm1Alee/Hspa5^tm1.1Alee Pten^tm1Hwu/Pten^tm1Hwu Tg(Pbsn-cre)4Prb/?	involves: 129 * 129S4/SvJae * C57BL/6 * DBA/2	MGI:4943512

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

embryonic lethality at implantation, complete penetrance ( J:111416 )

• most embryos survive to E3.5, while no homozygous embryos are recovered at E7.5: only empty deciduas are observed, indicating embryo degeneration and resorption shortly after E3.5

embryo

abnormal inner cell mass apoptosis ( J:111416 )

• there is a dramatic increase in apoptotic cell death in inner cell mass after 24 hours in culture compared to almost none in wild-type or heterozygous embryo culture

absent inner cell mass proliferation ( J:111416 )

• cultured E3.5 embryos fail to expand when cultured for 5 days after zona pellucida was mechanically removed

• after 1 day in culture, only 7% of cells show BrdU uptake vs 60% of wild-type cells

failure of blastocyst to hatch from the zona pellucida ( J:111416 )

abnormal blastocyst morphology ( J:111416 )

• after 96 hours in culture, blastocysts appear shrunken compared to wild-type

cellular

abnormal inner cell mass apoptosis ( J:111416 )

• there is a dramatic increase in apoptotic cell death in inner cell mass after 24 hours in culture compared to almost none in wild-type or heterozygous embryo culture

absent inner cell mass proliferation ( J:111416 )

• cultured E3.5 embryos fail to expand when cultured for 5 days after zona pellucida was mechanically removed

• after 1 day in culture, only 7% of cells show BrdU uptake vs 60% of wild-type cells

reproductive system

empty decidua capsularis ( J:111416 )

• only empty deciduas are observed

Allelic Composition	Hspa5^tm1Alee/Hspa5^tm1.1Alee Pten^tm1Hwu/Pten^tm1Hwu Tg(Pbsn-cre)4Prb/?
Genetic Background	involves: 129 * 129S4/SvJae * C57BL/6 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hspa5^tm1.1Alee mutation (1 available); any Hspa5 mutation (38 available)
Hspa5^tm1Alee mutation (0 available); any Hspa5 mutation (38 available)
Pten^tm1Hwu mutation (16 available); any Pten mutation (81 available)
Tg(Pbsn-cre)4Prb mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

neoplasm

neoplasm ( J:142106 )

N	• mice do not develop detectable cancerous or precancerous lesions in the prostate

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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