Phenotypes associated with this allele

• Allele Symbol: Igfbp3^tm1Jep
• Allele Name: targeted mutation 1, John E Pintar
• Allele ID: MGI:3663361
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Igfbp3^tm1Jep/Igfbp3^tm1Jep	involves: 129S/SvEv * C57BL/6J	MGI:3664117
cx2	Igfbp3^tm1Jep/Igfbp3^tm1Jep Igfbp4^tm1Jep/Igfbp4^tm1Jep Igfbp5^tm1Jep/Igfbp5^tm1Jep	involves: 129S/SvEv * C57BL/6J	MGI:3664121

Genotype
MGI:3664117

hm1

• Allelic Composition: Igfbp3^tm1Jep/Igfbp3^tm1Jep
• Genetic Background: involves: 129S/SvEv * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

vision/eye

abnormal retina vasculature morphology ( J:282246 )

• mice exhibit degenerate capillaries in the retinal vasculature

• mice treated with glycyrrhizin for 6 months show fewer degenerate capillaries

decreased retina ganglion cell number ( J:282246 )

• fewer cells in the ganglion cell layer

• mice treated with glycyrrhizin for 2 months show restoration of cell numbers in the ganglion cell layer

decreased total retina thickness ( J:282246 )

• thinner retinas

• mice treated with glycyrrhizin for 2 months show restoration of retinal thickness

nervous system

decreased retina ganglion cell number ( J:282246 )

• fewer cells in the ganglion cell layer

• mice treated with glycyrrhizin for 2 months show restoration of cell numbers in the ganglion cell layer

cellular

oxidative stress ( J:282246 )

• mice exhibit increased reactive oxygen species production at 2 and 6 months of age

• glycyrrhizin treatment for 6 months, but not 2 months, reduces reactive oxygen species levels

cardiovascular system

abnormal retina vasculature morphology ( J:282246 )

• mice exhibit degenerate capillaries in the retinal vasculature

• mice treated with glycyrrhizin for 6 months show fewer degenerate capillaries

growth/size/body

growth/size/body region phenotype ( J:112029 )

N • body weights of male and female mutants are indistinguishable from controls from birth to 42 days of age

Genotype
MGI:3664121

cx2

• Allelic Composition: Igfbp3^tm1Jep/Igfbp3^tm1Jep; Igfbp4^tm1Jep/Igfbp4^tm1Jep; Igfbp5^tm1Jep/Igfbp5^tm1Jep
• Genetic Background: involves: 129S/SvEv * C57BL/6J

growth/size/body

decreased body weight ( J:112029 )

• mice exhibit significantly greater growth retardation than Igfbp4-null mice becoming significant at P20 (78% of normal weight) and continues through week 14

homeostasis/metabolism

decreased circulating insulin-like growth factor I level ( J:112029 )

• mutants have a 50% reduction in total serum Igf-1 compared to wild-type; Igf-1 bioactivity is significantly suppressed in mutants

• mutants show lower circulating Igf-1 levels than wild-type controls at all time points examined after glucose challenge; percentage increase in Igf-1 levels after challenge is significantly higher in mutants compared to wild-type at 30 minutes (43%) while wild-type only show a 6% increase at 30 minutes and 8% at 60 minutes

abnormal glucose homeostasis ( J:112029 )

• after an overnight fast, mutants have significantly reduced glucose levels vs wild-type mice

increased insulin secretion ( J:112029 )

• mice show enhanced insulin secretion after glucose challenge

decreased circulating glucose level ( J:112029 )

• mutants show significantly decreased glucose levels after glucose challenge (from 30-120 minutes) compared to wild-type or Igfbp3 or Igfbp5 mutants; decrease is significantly enhanced compared to Igfbp4 mutants

• after an overnight fast, mutants have significantly reduced glucose levels vs wild-type mice

increased circulating insulin level ( J:112029 )

• mutants show increased circulating insulin levels after glucose challenge that are significantly higher than the increase seen in wild-type after glucose challenge

abnormal enzyme/coenzyme activity ( J:112029 )

• mutants show slightly increased AKT activation levels and significantly decreased Erk/MAPK (72% decreased) activation levels vs wild-type

endocrine/exocrine glands

abnormal pancreatic beta cell morphology ( J:112029 )

• islets in mutants have increased area; beta-cell area is increased ~37% compared to wild-type

increased insulin secretion ( J:112029 )

• mice show enhanced insulin secretion after glucose challenge

adipose tissue

abnormal abdominal fat pad morphology ( J:112029 )

• pads of mutants contain much smaller adipocytes than in wild-type fat pads

decreased total fat pad weight ( J:112029 )

• weights of all fat pads are significantly decreased in mutants

muscle

decreased quadriceps weight ( J:112029 )

• quadriceps muscle is decreased in weight compared to controls

limbs/digits/tail

decreased quadriceps weight ( J:112029 )

• quadriceps muscle is decreased in weight compared to controls