About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Chd4tm1.2Kge
targeted mutation 1.2, Katia Georgopoulos
MGI:3641409
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Chd4tm1.1Kge/Chd4tm1.2Kge
Tg(CAG-Bgeo/GFP)21Lbe/0
Tg(Lck-cre)1Cwi/0 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:3713384
cn2
 		Chd4tm1.2Kge/Chd4tm1.2Kge
Tg(Lck-cre)1Cwi/0 		involves: C57BL/6 * DBA/2 * SJL MGI:3653117
cn3
 		Chd4tm1.1Kge/Chd4tm1.2Kge
Tg(Lck-cre)1Cwi/0 		involves: C57BL/6 * SJL MGI:3713382


Genotype
MGI:3713384
cn1
Allelic
Composition		 Chd4tm1.1Kge/Chd4tm1.2Kge
Tg(CAG-Bgeo/GFP)21Lbe/0
Tg(Lck-cre)1Cwi/0
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chd4tm1.1Kge mutation (0 available); any Chd4 mutation (58 available)
Chd4tm1.2Kge mutation (0 available); any Chd4 mutation (58 available)
Tg(CAG-Bgeo/GFP)21Lbe mutation (2 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal cell cycle ( J:109547 )
• progressive decrease in cycling T cells is observed from second to third cell division in vitro, along with large number of nonblast T cells that appear to fail to enter cell cycle
decreased T cell proliferation ( J:109547 )
• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase

immune system
decreased T cell proliferation ( J:109547 )
• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase

hematopoietic system
decreased T cell proliferation ( J:109547 )
• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase




Genotype
MGI:3653117
cn2
Allelic
Composition		 Chd4tm1.2Kge/Chd4tm1.2Kge
Tg(Lck-cre)1Cwi/0
Genetic
Background		 involves: C57BL/6 * DBA/2 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chd4tm1.2Kge mutation (0 available); any Chd4 mutation (58 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased T cell proliferation ( J:109547 )
• in vitro, TCR/CD3 stimulation shows a 5-7 fold decrease in proliferating T cells compared to controls, specifically CD8+CD4lo and CD8+ CD4- populations
thymus hypoplasia ( J:109547 )
• null mice exhibit a consistent reduction in cellularity compared to wild-type or heterozygotes
increased double-negative T cell number ( J:109547 )
• there is an increase in the population of the T cells in the late double negative (DN4) stage in the neonatal thymus
decreased double-positive T cell number ( J:109547 )
• percentage of double positive thymocytes is severely reduced
increased CD8-positive, alpha-beta T cell number ( J:109547 )
• CD8+ cells are greatly increased in number
• CD8+ populations in the thymus express TCR levels more similar to double-positive cells rather than intermediate single-positive or single-positive levels

hematopoietic system
decreased T cell proliferation ( J:109547 )
• in vitro, TCR/CD3 stimulation shows a 5-7 fold decrease in proliferating T cells compared to controls, specifically CD8+CD4lo and CD8+ CD4- populations
thymus hypoplasia ( J:109547 )
• null mice exhibit a consistent reduction in cellularity compared to wild-type or heterozygotes
increased double-negative T cell number ( J:109547 )
• there is an increase in the population of the T cells in the late double negative (DN4) stage in the neonatal thymus
decreased double-positive T cell number ( J:109547 )
• percentage of double positive thymocytes is severely reduced
increased CD8-positive, alpha-beta T cell number ( J:109547 )
• CD8+ cells are greatly increased in number
• CD8+ populations in the thymus express TCR levels more similar to double-positive cells rather than intermediate single-positive or single-positive levels

cellular
abnormal cell cycle ( J:109547 )
• activated T cells are unable to go through normal cell divisions
decreased T cell proliferation ( J:109547 )
• in vitro, TCR/CD3 stimulation shows a 5-7 fold decrease in proliferating T cells compared to controls, specifically CD8+CD4lo and CD8+ CD4- populations

endocrine/exocrine glands
thymus hypoplasia ( J:109547 )
• null mice exhibit a consistent reduction in cellularity compared to wild-type or heterozygotes




Genotype
MGI:3713382
cn3
Allelic
Composition		 Chd4tm1.1Kge/Chd4tm1.2Kge
Tg(Lck-cre)1Cwi/0
Genetic
Background		 involves: C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chd4tm1.1Kge mutation (0 available); any Chd4 mutation (58 available)
Chd4tm1.2Kge mutation (0 available); any Chd4 mutation (58 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
thymus hypoplasia ( J:109547 )
• reduced in mutant mice

immune system
thymus hypoplasia ( J:109547 )
• reduced in mutant mice

endocrine/exocrine glands
thymus hypoplasia ( J:109547 )
• reduced in mutant mice




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory