Analysis Tools
mortality/aging
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• between E10.5 and E11.5, about 1 day longer than null mice without the transgene
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cardiovascular system
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• a compact nonvascularized cell layer is seen in place of the normal capillary bed
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• seen at E9.5; however no increase in apoptosis is seen in the myocardium unlike in null mice without the transgene
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• at E9.0 and E10.5, heart rate is reduced to about 46 and 57 bpm compared to 72 and 76 bpm in wild-type but increased compared to null mice without the transgene (about 4 and 0 bpm)
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embryo
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• a compact nonvascularized cell layer is seen in place of the normal capillary bed
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• begins around E9.0; however, the increased number of somites and presence of a forelimb bud at E10.5 indicate that the partially rescued embryos develop beyond E9.0 when null embryos without the transgene stop developing
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• the mesoderm and endoderm are separate with few areas of contact
• at E9.5-E10.5, the yolk sac is wrinkled, shrunken, and anemic
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• no definitive vasculature is present at E9.5; however some primitive vascular cells are present and hematopoiesis is normal
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growth/size/body
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• begins around E9.0; however, the increased number of somites and presence of a forelimb bud at E10.5 indicate that the partially rescued embryos develop beyond E9.0 when null embryos without the transgene stop developing
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