Phenotypes associated with this allele

• Allele Symbol: Ly9^tm1Mckn
• Allele Name: targeted mutation 1, David J McKean
• Allele ID: MGI:3618122
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ly9^tm1Mckn/Ly9^tm1Mckn	involves: 129/Sv * C57BL/6	MGI:3618124

Genotype
MGI:3618124

hm1

• Allelic Composition: Ly9^tm1Mckn/Ly9^tm1Mckn
• Genetic Background: involves: 129/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased T cell proliferation ( J:105482 )

• homozygous mutant splenocytes stimulated in vitro with anti-CD3 antibody proliferated significantly less than did those from wild-type mice

abnormal T-helper 2 cell morphology ( J:105482 )

• following in vitro stimulation with low doses of anti-T cell receptor (anti-TCR) and anti-CD28 antibodies, the response of purified mutant splenic CD4⁺ T cells previously cultured under Th1/Th2 neutral conditions was skewed toward Th1, as indicated by their reduced expression, relative to wild-type cells, of IL-4; mutant and wild-type CD4⁺ T cells' responses were indistinguishable if stimulation followed preculture under Th1 or Th2 polarizing conditionss, and optimization of anti-TCR/anti-CD28 doses reduced the response difference to insignificance

abnormal cytokine secretion ( J:105482 )

• homozygous mutant splenocytes stimulated in vitro with anti-CD3 antibody produced significantly less interleukin 2 (IL2) than did those from wild-type mice

hematopoietic system

decreased T cell proliferation ( J:105482 )

• homozygous mutant splenocytes stimulated in vitro with anti-CD3 antibody proliferated significantly less than did those from wild-type mice

abnormal T-helper 2 cell morphology ( J:105482 )

• following in vitro stimulation with low doses of anti-T cell receptor (anti-TCR) and anti-CD28 antibodies, the response of purified mutant splenic CD4⁺ T cells previously cultured under Th1/Th2 neutral conditions was skewed toward Th1, as indicated by their reduced expression, relative to wild-type cells, of IL-4; mutant and wild-type CD4⁺ T cells' responses were indistinguishable if stimulation followed preculture under Th1 or Th2 polarizing conditionss, and optimization of anti-TCR/anti-CD28 doses reduced the response difference to insignificance

cellular

decreased T cell proliferation ( J:105482 )

• homozygous mutant splenocytes stimulated in vitro with anti-CD3 antibody proliferated significantly less than did those from wild-type mice