Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Scn9atm1Dgen mutation
(1 available);
any
Scn9a mutation
(120 available)
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mortality/aging
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• Background Sensitivity: no viable mice are obtained on a congenic C57BL/6 background, indicating neonatal lethality, compared to 20% survival on mixed backgrounds
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Scn9atm1Dgen mutation
(1 available);
any
Scn9a mutation
(120 available)
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mortality/aging
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• Background Sensitivity: nurturing of neonates on the congenic BALB/c background does not improve survival as it does on a mixed backgrounds
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Allelic
Composition |
Scn9atm1Dgen/Scn9atm1Dgen
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Genetic
Background |
either: (involves: 129P2/OlaHsd * BALB/c * C57BL/6) or (involves: 129P2/OlaHsd * C57BL/6 * CD-1) |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Scn9atm1Dgen mutation
(1 available);
any
Scn9a mutation
(120 available)
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mortality/aging
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• Background Sensitivity: most mice on a mixed BALB/c background die before P14 while most mice on a mixed CD-1 background die before P20 compared to complete neonatal lethality of mice on a congenic C57BL/6 or BALB/c background
• Background Sensitivity: mice on either a mixed BALB/c or CD-1 background show an approximate 20% survival rate to adulthood when neonates are nurtured (by hand feeding, by receiving supplementary dextrose injections when dehydrated, and by culling litters to prevent competition for resources)
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growth/size/body
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• neonates are smaller than wild-type mice, however mice that survive to adulthood show normal body size
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behavior/neurological
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• in the tail-clip assay, mutants are insensitive to mechanical hyperalgesia and do not react to a clip placed on the tail
• however, mice show unchanged tactile sensitivity measured with von Frey fibers
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• mice lack a response to histamine, showing few to no scratching bouts in response to histamine injection, although a classic flare response at the injection site is seen
• 24 hours after injection of complete Freunds adjuvant into the paw, mutants do not develop thermal hyperalgesia at any time point measured in the Hargreaves apparatus as seen in controls
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• mice do not respond to nociceptive stimuli
• however, mice exhibit normal overall locomotion and rearing
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• mice exhibit reduced responses in both phases of the formalin pain assay, with licking and lifting in phase I of the formalin response being about 80% less in mutants and little to no licking and lifting was seen in phase II
• mice treated with veratradine or grayanotoxin III show reduced lifting and licking response, with an 85% and 96% lower response, respectively, than controls indicating that mice are insensitive to pain resulting from activation of peripheral sodium channels
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• in the hot plate test, latency to withdrawal at each temperature is longer in mutants and mice do not display painful behaviors
• in the Hargreaves apparatus, mutant mice react with an approximate 40% increase in latency time compared to controls
• 24 hours after injection of complete Freunds adjuvant into the paw, mutants do not develop thermal hyperalgesia at any time point measured in the Hargreaves apparatus as seen in controls
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immune system
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• Background Sensitivity: in the early stages of production where F1 and F2 mice are bred, mice often show dermatitis
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integument
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• Background Sensitivity: in the early stages of production where F1 and F2 mice are bred, mice often show dermatitis
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nervous system
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• C-fiber spontaneous action potential frequency is 6-fold lower in saphenous nerve
• C-fiber action potential frequency is reduced in response to mechanical stimuli at the higher spiking rates evoked by stronger stimulus
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• mice show reduced tetrodotoxin-sensitive sodium current recorded from sensory neuron cell bodies
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renal/urinary system
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• Background Sensitivity: in the early stages of production where F1 and F2 mice are bred, some mice, mainly males, present with urinary issues such as prolapses and blockage
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taste/olfaction
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• in a buried food assay, 18 of 19 mice do not find the hidden food pellet
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Scn9atm1Dgen mutation
(1 available);
any
Scn9a mutation
(120 available)
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mortality/aging
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• grossly normal homozygous embryos are identified at E12.5 to E18.5, but not at later stages
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Allelic
Composition |
Scn9atm1Dgen/Scn9a+
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Genetic
Background |
involves: 129P2/OlaHsd * C57BL/6 |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Scn9atm1Dgen mutation
(1 available);
any
Scn9a mutation
(120 available)
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nervous system
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• exhibit significantly increased prepulse inhibition compared with age- and gender-matched wildtype controls
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Analysis Tools
