Phenotypes associated with this allele

• Allele Symbol: Hmgn1^tm1Mbus
• Allele Name: targeted mutation 1, Michael Bustin
• Allele ID: MGI:3590648
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hmgn1^tm1Mbus/Hmgn1^tm1Mbus	either: (involves: 129/Sv * 129X1/SvJ) or (involves: 129/Sv * 129X1/SvJ * C57BL/6)	MGI:3617360
hm2	Hmgn1^tm1Mbus/Hmgn1^tm1Mbus	involves: 129X1/SvJ	MGI:3617361
ht3	Hmgn1^tm1Mbus/Hmgn1^+	either: (involves: 129/Sv * 129X1/SvJ) or (involves: 129/Sv * 129X1/SvJ * C57BL/6)	MGI:3617364
ht4	Hmgn1^tm1Mbus/Hmgn1^+	involves: 129X1/SvJ	MGI:3617363

Genotype
MGI:3617360

hm1

• Allelic Composition: Hmgn1^tm1Mbus/Hmgn1^tm1Mbus
• Genetic Background: either: (involves: 129/Sv * 129X1/SvJ) or (involves: 129/Sv * 129X1/SvJ * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, incomplete penetrance ( J:100282 )

• at E11.5 only 8% of mice are homozygous rather than the expected 25%

cellular

abnormal cell physiology ( J:100282 )

• in primary MEFs, the removal of UV induced cyclobutane pyrimidine dimers is slower than in wild-type cells

increased cellular sensitivity to ultraviolet irradiation ( J:100282 )

• primary MEFs are more sensitive to UV-C irradiation induced death (D50 = 3 J/m2 compared to 13.5 J/m2 for wild-type cells)

integument

increased sensitivity to skin irradiation ( J:100282 )

• a cumulative UV-B dose of 1200 J/m2 produces skin alterations including acanthosis and localized hyperkeratosis in homozygotes but not in wild-type mice

Genotype
MGI:3617361

hm2

• Allelic Composition: Hmgn1^tm1Mbus/Hmgn1^tm1Mbus
• Genetic Background: involves: 129X1/SvJ

mortality/aging

increased mortality induced by gamma-irradiation ( J:100773 )

• 1 year after sublethal gamma-irradiation only 45% of homozygous mice survive compared to 75% of wild-type littermates

neoplasm

increased metastatic potential ( J:100773 )

• at 1 year of age 25% of male homozygotes had malignant tumors that metastasized compared to 0% of wild-type males

increased tumor incidence ( J:100773 )

• at 1 year of age over 40% of homozygotes have multiple tumors compared to 17% and 27% of wild-type males and females, respectively

• life span and average age of tumor detection are not different from wild-type; however in mice that die young tumors were detected at significantly younger ages in homozygotes compared to wild-type mice

• a marginal increase in the incidence of hematopoietic and hepatic neoplasms, as well as occurrence of unusual tumors (granule cell tumors of the brain, jejunal carcinoma, and renal tubule neoplasms) are seen

increased pheochromocytoma incidence ( J:100773 )

• the frequency of endocrine tumors, including adrenal pheochromocytoma, are increased

increased hemangioma incidence ( J:100773 )

• a marginal increase in the incidence of hemangiosarcomas is seen

increased malignant tumor incidence ( J:100773 )

• at 1 year of age 58% of male and 82% of female homozygotes have malignant tumors compared to 29% and 55% of wild-type males and females, respectively

increased incidence of tumors by ionizing radiation induction ( J:100773 )

• of the mice that die within 1 year 90% have tumors usually a large thymic mass indicating an increase in the incidence of lymphomas

cellular

abnormal cell cycle checkpoint function ( J:100773 )

• 1 hour after gamma-irradiation with 0.6 Gy homozygous cells do not delay entry into mitosis unlike wild-type cells

increased cellular sensitivity to gamma-irradiation ( J:100773 )

• primary MEFs are more sensitive to gamma-irradiation induced death (D50 = 3.5 Gy compared to greater than 7 Gy for wild-type cells)

increased fibroblast proliferation ( J:100773 )

• the doubling time of primary MEFs is only 12 hours compared to 19 hours in wild-type cells

• homozygous cells reach senescence at passage 13 rather than passage 8 as in wild-type cells

homeostasis/metabolism

decreased circulating corticosterone level ( J:199604 )

♀ • in female mice

increased mortality induced by gamma-irradiation ( J:100773 )

• 1 year after sublethal gamma-irradiation only 45% of homozygous mice survive compared to 75% of wild-type littermates

behavior/neurological

decreased startle reflex ( J:199604 )

• lower acoustic startle activity

decreased vertical activity ( J:199604 )

• in an open field

nervous system

decreased prepulse inhibition ( J:199604 )

• lower acoustic startle activity

endocrine/exocrine glands

increased pheochromocytoma incidence ( J:100773 )

• the frequency of endocrine tumors, including adrenal pheochromocytoma, are increased

Genotype
MGI:3617364

ht3

• Allelic Composition: Hmgn1^tm1Mbus/Hmgn1⁺
• Genetic Background: either: (involves: 129/Sv * 129X1/SvJ) or (involves: 129/Sv * 129X1/SvJ * C57BL/6)

cellular

increased cellular sensitivity to ultraviolet irradiation ( J:100282 )

• primary MEFs are more sensitive to UV-C irradiation induced death compared to wild-type cells but less sensitive than homozygous cells

Genotype
MGI:3617363

ht4

• Allelic Composition: Hmgn1^tm1Mbus/Hmgn1⁺
• Genetic Background: involves: 129X1/SvJ

mortality/aging

increased mortality induced by gamma-irradiation ( J:100773 )

• 1 year after sublethal gamma-irradiation only 49% of heterozygous mice survive compared to 75% of wild-type littermates

cellular

increased cellular sensitivity to gamma-irradiation ( J:100773 )

• primary MEFs are more sensitive to gamma-irradiation induced death compared to wild-type cells but less sensitive than homozygous cells

homeostasis/metabolism

increased mortality induced by gamma-irradiation ( J:100773 )

• 1 year after sublethal gamma-irradiation only 49% of heterozygous mice survive compared to 75% of wild-type littermates