Phenotypes associated with this allele

• Allele Symbol: Dad1^tm1Kni
• Allele Name: targeted mutation 1, Kiyomasa Nishii
• Allele ID: MGI:3586491
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dad1^tm1Kni/Dad1^tm1Kni	involves: 129S/SvEv	MGI:3586725
ht2	Dad1^tm1Kni/Dad1^+	involves: 129S/SvEv	MGI:3586726

Genotype
MGI:3586725

hm1

• Allelic Composition: Dad1^tm1Kni/Dad1^tm1Kni
• Genetic Background: involves: 129S/SvEv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:99897 )

• homozygous embryos die between E6.5 and E7.5

embryo

embryonic growth retardation ( J:99897 )

• at E6.5 embryos are small and lack a well-defined distinction between embryonic and extra-embryonic tissues

• in culture about 50% of homozygous embryos initially grow normally but detach from the culture dish after 2-5 days, the other half display growth impairment

growth/size/body

embryonic growth retardation ( J:99897 )

• at E6.5 embryos are small and lack a well-defined distinction between embryonic and extra-embryonic tissues

• in culture about 50% of homozygous embryos initially grow normally but detach from the culture dish after 2-5 days, the other half display growth impairment

Genotype
MGI:3586726

ht2

• Allelic Composition: Dad1^tm1Kni/Dad1⁺
• Genetic Background: involves: 129S/SvEv

mortality/aging

embryonic lethality between implantation and somite formation, incomplete penetrance ( J:99897 )

• about 20% of heterozygous embryos are lost by E8.5

embryo

embryonic growth retardation ( J:99897 )

• in culture, 4 of 21 heterozygous embryos displayed impaired growth

immune system

decreased thymus weight ( J:99897 )

• the thymus weighs about 10% less than in wild-type mice; however, no difference in body weight is detected

limbs/digits/tail

syndactyly ( J:99897 )

• soft tissue syndactyly, generally as webbing between the second and third digits, is seen in 14% of heterozygotes on the hindlimbs and in 5% of heterozygotes on the forelimbs

• all pups with forelimb syndactyly also have hindlimb deformities

hematopoietic system

decreased thymus weight ( J:99897 )

• the thymus weighs about 10% less than in wild-type mice; however, no difference in body weight is detected

growth/size/body

embryonic growth retardation ( J:99897 )

• in culture, 4 of 21 heterozygous embryos displayed impaired growth

endocrine/exocrine glands

decreased thymus weight ( J:99897 )

• the thymus weighs about 10% less than in wild-type mice; however, no difference in body weight is detected