Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rps12em1Nbakr mutation
(1 available);
any
Rps12 mutation
(44 available)
Tg(Tal1-cre/ERT)42-056Jrg mutation
(2 available)
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hematopoietic system
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• in tamoxifen-treated mice
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• in tamoxifen-treated mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Adartm1.1Phs mutation
(2 available);
any
Adar mutation
(71 available)
Adartm2Phs mutation
(0 available);
any
Adar mutation
(71 available)
Tg(Tal1-cre/ERT)42-056Jrg mutation
(2 available)
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hematopoietic system
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• bone marrow cellularity is reduced in mice after cre induction
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• the frequency of Lin- c-Kit+Sca-1- hematopoietic stem cells (HSC) in the bone marrow after cre induction is much lower than controls
• the frequency of Lin- c-Kit+Sca-1+ HSC in the bone marrow after cre induction is much higher than controls
• 68% of actively cycling HSCs are apoptotic after cre induction versus 3-13% in control mice
• the bone marrow has many fewer Lin- c-Kit+Sca-1+CD34lo HSC cells
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hif1atm3Rsjo mutation
(3 available);
any
Hif1a mutation
(48 available)
Tg(Tal1-cre/ERT)42-056Jrg mutation
(2 available)
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hematopoietic system
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• in tamoxifen-treated mice
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• tamoxifen-treated mice exhibit an increase in B lymphoid cells in the bone marrow compared with control mice
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• tamoxifen-treated mice exhibit an increase in T lymphoid cells in the bone marrow compared with control mice
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• in tamoxifen-treated mice
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• long-term hematopoietic stem cells from tamoxifen-treated mice exhibit increased oxygen consumption rates and lower levels of lactate indicating decreased cytoplasmic glycolysis compared with control cells
• long-term hematopoietic stem cells from tamoxifen-treated mice exhibit loss of quiescence compared with control cells
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immune system
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• tamoxifen-treated mice exhibit an increase in B lymphoid cells in the bone marrow compared with control mice
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• tamoxifen-treated mice exhibit an increase in T lymphoid cells in the bone marrow compared with control mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Meis1tm2Ngc mutation
(1 available);
any
Meis1 mutation
(46 available)
Tg(Tal1-cre/ERT)42-056Jrg mutation
(2 available)
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hematopoietic system
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• tamoxifen-treated mice exhibit reduction in granulocyte-monocyte progenitor (GMPs) and common myeloid progenitor (CMPs) cells and mild reduction in the total number of megakaryocyte/erythroid progenitor cells (MEP) compared with control mice
• however, tamoxifen-treated mice exhibit normal percentage of differentiated myeloid progenitor cells and erythroid progenitor cells and BFU-E and total number of colonies are rescued by re-expression of Hif1a
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• tamoxifen-treated mice exhibit reduced common myeloid progenitors and primitive myeloid progenitors compared with control mice
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• increased frequency in tamoxifen-treated mice
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• long-term hematopoietic stem cells are less quiescent, prone to proliferation and more apoptotic in tamoxifen-treated mice than in control mice
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cellular
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• increased reactive oxygen species in the long-term hematopoietic stem cells of tamoxifen-treated mice
• however, treatment with N-acetyl-L-cysteine (NAC) restores reactive oxygen species
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Analysis Tools