Phenotypes associated with this allele

• Allele Symbol: Tg(Col1a1-cre)2Bek
• Allele Name: transgene insertion 2, Barbara E Kream
• Allele ID: MGI:3574763
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Casr^tm1Wch/Casr^tm1Wch Tg(Col1a1-cre)2Bek/0	involves: 129S4/SvJae * C57BL/6 * CD-1	MGI:5306891
cn2	Fam20c^tm1.1Cqi/Fam20c^tm1.1Cqi Tg(Col1a1-cre)2Bek/0	involves: 129S6/SvEvTac * CD-1	MGI:5755483
cn3	Kitl^tm2.1Sjm/Kitl^tm2.2Sjm Tg(Col1a1-cre)2Bek/0	involves: BALB/cJ * C57BL/6 * C57BL/Ka * CD-1 * SJL	MGI:5306352
cn4	Cxcl12^tm1.1Sjm/Cxcl12^tm1.1Sjm Tg(Col1a1-cre)2Bek/?	involves: C57BL/6 * CD-1	MGI:5488611
cn5	Npy1r^tm1Wpg/Npy1r^tm1Wpg Tg(Col1a1-cre)2Bek/0	involves: CD-1	MGI:4946407

Genotype
MGI:5306891

cn1

• Allelic Composition: Casr^tm1Wch/Casr^tm1Wch; Tg(Col1a1-cre)2Bek/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CD-1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:180651 )

• mice die within 3 weeks of birth

skeleton

abnormal skeleton morphology ( J:180651 )

• small, fragile, and undermineralized skeleton

decreased bone mineral density ( J:180651 )

decreased compact bone volume ( J:180651 )

decreased compact bone thickness ( J:180651 )

abnormal trabecular bone morphology ( J:180651 )

• mice exhibit increased trabecular spacing compared with control mice

decreased trabecular bone volume ( J:180651 )

decreased bone trabecula number ( J:180651 )

decreased trabecular bone connectivity density ( J:180651 )

decreased trabecular bone thickness ( J:180651 )

abnormal skeleton development ( J:180651 )

• blocked

decreased bone mineralization ( J:180651 )

• undermineralized skeleton

fragile skeleton ( J:180651 )

growth/size/body

decreased body size ( J:180651 )

• at 7 day and 21 days

decreased body weight ( J:180651 )

postnatal growth retardation ( J:180651 )

• blocked

Genotype
MGI:5755483

cn2

• Allelic Composition: Fam20c^tm1.1Cqi/Fam20c^tm1.1Cqi; Tg(Col1a1-cre)2Bek/0
• Genetic Background: involves: 129S6/SvEvTac * CD-1

digestive/alimentary system

detached junctional epithelium ( J:225679 )

• periodontium shows detachment of the junctional epithelium in 12 and 24 week old mice

skeleton

alveolar process atrophy ( J:225679 )

• the interdental region between the first and second mandibular molars shows bone loss at 12 weeks of age and by 24 weeks of age, very little alveolar bone remains in the interdental region

• at 24 weeks of age, the alveolar bone in the furcation region becomes island-like (bone spicules)

abnormal periodontal ligament morphology ( J:225679 )

• at 4 weeks of age, collagen fibers in the periodontal ligament are thinner and more disorganized and some collagen fibers appear broken or detached from the alveolar bone or root surface

• inflammation is seen in the periodontal ligament of 12 and 24 week old mice

periodontal ligament necrosis ( J:225679 )

• by 24 weeks of age, certain areas of the periodontal ligament are necrotized, accompanied by formation of abscesses due to bone absorption

abnormal cementum morphology ( J:225679 )

• amounts of cellular cementum appear to be reduced in 4, 12 and 24 week old mice

tooth abscess ( J:225679 )

• by 24 weeks of age, certain areas of the periodontal ligament are necrotized, accompanied by formation of abscesses due to bone absorption

dental pulp inflammation ( J:225679 )

• inflammation in the dental pulp

decreased bone mineral density ( J:225679 )

• alveolar bone in the furcation and interdental regions shows a lower mineral density at 4 weeks of age, it appears more porous at 12 weeks of age, and abnormalities worsen with age, with 24 week old mice showing severe defects and porosities in the alveolar bone

decreased bone volume ( J:225679 )

• reduction in alveolar bone volume at 12 and 24 week of age

abnormal osteocyte lacunocanalicular system morphology ( J:225679 )

• at 4 weeks of age, the lacuno-canalicular networks in alveolar bone appear disorganized

abnormal osteocyte morphology ( J:225679 )

• lacunae of osteocytes in the alveolar bone of the interdental or furcation regions appear larger and are irregularly distributed with fewer organized canaliculi

abnormal osteocyte dendritic process morphology ( J:225679 )

• osteocytes processes in the alveolar bone appear abnormal

abnormal osteocyte canaliculus morphology ( J:225679 )

• fewer organized canaliculi are observed in alveolar bone; canaliculi appear thicker and more randomly distributed

abnormal osteocyte lacuna morphology ( J:225679 )

• the lacunae of osteocytes appeared "collapsed"

immune system

dental pulp inflammation ( J:225679 )

• inflammation in the dental pulp

periodontium inflammation ( J:225679 )

• mice develop periodontal disease, showing increased inflammation in the interdental and furcation regions

• inflammation is seen in the periodontal ligament of 12 and 24 week old mice

craniofacial

alveolar process atrophy ( J:225679 )

• the interdental region between the first and second mandibular molars shows bone loss at 12 weeks of age and by 24 weeks of age, very little alveolar bone remains in the interdental region

• at 24 weeks of age, the alveolar bone in the furcation region becomes island-like (bone spicules)

abnormal periodontal ligament morphology ( J:225679 )

• at 4 weeks of age, collagen fibers in the periodontal ligament are thinner and more disorganized and some collagen fibers appear broken or detached from the alveolar bone or root surface

• inflammation is seen in the periodontal ligament of 12 and 24 week old mice

periodontal ligament necrosis ( J:225679 )

• by 24 weeks of age, certain areas of the periodontal ligament are necrotized, accompanied by formation of abscesses due to bone absorption

abnormal cementum morphology ( J:225679 )

• amounts of cellular cementum appear to be reduced in 4, 12 and 24 week old mice

tooth abscess ( J:225679 )

• by 24 weeks of age, certain areas of the periodontal ligament are necrotized, accompanied by formation of abscesses due to bone absorption

detached junctional epithelium ( J:225679 )

• periodontium shows detachment of the junctional epithelium in 12 and 24 week old mice

periodontal pocket ( J:225679 )

• 12 and 24 week old mice show formation of periodontal pockets

growth/size/body

alveolar process atrophy ( J:225679 )

• the interdental region between the first and second mandibular molars shows bone loss at 12 weeks of age and by 24 weeks of age, very little alveolar bone remains in the interdental region

• at 24 weeks of age, the alveolar bone in the furcation region becomes island-like (bone spicules)

abnormal periodontal ligament morphology ( J:225679 )

• at 4 weeks of age, collagen fibers in the periodontal ligament are thinner and more disorganized and some collagen fibers appear broken or detached from the alveolar bone or root surface

• inflammation is seen in the periodontal ligament of 12 and 24 week old mice

periodontal ligament necrosis ( J:225679 )

• by 24 weeks of age, certain areas of the periodontal ligament are necrotized, accompanied by formation of abscesses due to bone absorption

abnormal cementum morphology ( J:225679 )

• amounts of cellular cementum appear to be reduced in 4, 12 and 24 week old mice

tooth abscess ( J:225679 )

• by 24 weeks of age, certain areas of the periodontal ligament are necrotized, accompanied by formation of abscesses due to bone absorption

detached junctional epithelium ( J:225679 )

• periodontium shows detachment of the junctional epithelium in 12 and 24 week old mice

periodontal pocket ( J:225679 )

• 12 and 24 week old mice show formation of periodontal pockets

decreased body height ( J:225679 )

• small stature

homeostasis/metabolism

decreased circulating phosphate level ( J:225679 )

• about 50% reduction in serum phosphorus levels

decreased collagen level ( J:225679 )

• the majority of collagen fibers in the interdental area are lost in 12 week old mice and nearly all of the collagen fibers in the interdental region are broken down at 24 weeks of age

Genotype
MGI:5306352

cn3

• Allelic Composition: Kitl^tm2.1Sjm/Kitl^tm2.2Sjm; Tg(Col1a1-cre)2Bek/0
• Genetic Background: involves: BALB/cJ * C57BL/6 * C57BL/Ka * CD-1 * SJL

hematopoietic system

hematopoietic system phenotype ( J:180431 )

N • exhibit normal blood cell counts, bone marrow composition, bone marrow and spleen cellularity, and reconstitution capacity

decreased hematopoietic cell number ( J:180431 )

• 2-fold as in Kitl^tm2.2Sjm heterozygotes in the bone marrow

Genotype
MGI:5488611

cn4

• Allelic Composition: Cxcl12^tm1.1Sjm/Cxcl12^tm1.1Sjm; Tg(Col1a1-cre)2Bek/?
• Genetic Background: involves: C57BL/6 * CD-1

hematopoietic system

decreased bone marrow cell number ( J:194748 )

• reduced CLP and IL7RalphaLMPP cells

abnormal bone marrow cell physiology ( J:194748 )

• effectiveness of donor reconstitution of T and B cells is reduced

Genotype
MGI:4946407

cn5

• Allelic Composition: Npy1r^tm1Wpg/Npy1r^tm1Wpg; Tg(Col1a1-cre)2Bek/0
• Genetic Background: involves: CD-1

skeleton

abnormal osteoblast physiology ( J:170291 )

• mice exhibit increased osteoblast activity compared to in wild-type mice

increased diameter of femur ( J:170291 )

• with an increase in total tissue area, marrow area, periosteal perimeter and endosteal perimeter

increased bone mineral content ( J:170291 )

• whole body and lumbar vertebrae

increased bone mineral density ( J:170291 )

increased bone trabecula number ( J:170291 )

increased trabecular bone thickness ( J:170291 )

abnormal bone ossification ( J:170291 )

• bone formation rate in cancellous and cortical bone is increased compared to in wild-type mice

increased bone strength ( J:170291 )

limbs/digits/tail

increased diameter of femur ( J:170291 )

• with an increase in total tissue area, marrow area, periosteal perimeter and endosteal perimeter

cellular

abnormal osteoblast physiology ( J:170291 )

• mice exhibit increased osteoblast activity compared to in wild-type mice