Phenotypes associated with this allele

• Allele Symbol: Dlk1^tm1Srba
• Allele Name: targeted mutation 1, Steven R Bauer
• Allele ID: MGI:3526402
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dlk1^tm1Srba/Dlk1^tm1Srba	involves: 129X1/SvJ * C57BL/6	MGI:3838139
ht2	Dlk1^tm1Srba/Dlk1^+	involves: 129X1/SvJ * C57BL/6	MGI:3838138

Genotype
MGI:3838139

hm1

• Allelic Composition: Dlk1^tm1Srba/Dlk1^tm1Srba
• Genetic Background: involves: 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased body size ( J:145076 )

• mice have growth retardation compared to controls

immune system

abnormal B cell number ( J:145076 )

decreased immature B cell number ( J:145076 )

• immature "Fraction C" pro-B cell numbers in the bone marrow are slightly decreased at 6- and 8- weeks of age

increased immature B cell number ( J:145076 )

• immature "Fraction A" pro-B cell numbers in the bone marrow are almost double that of controls at 4 weeks of age

• culturing bone marrow cells with IL-7 leads to 75% development of "Fraction C" pro-B cells compared to 50% in controls

increased transitional stage B cell number ( J:145076 )

• T1 transitional B cell numbers are increased 6-fold in the spleen of 8 week old mice

decreased mature B cell number ( J:145076 )

• the number of recirculating mature B cells in the bone marrow of 8 week old mice is about half that of controls

abnormal spleen B cell follicle morphology ( J:145076 )

• spleen follicular zone areas are slightly but significantly decreased

abnormal spleen marginal zone morphology ( J:145076 )

• marginal zones are 1.8-fold larger in area than controls

increased marginal zone B cell number ( J:145076 )

• marginal zone B cell numbers are 4-fold higher than controls

increased immunoglobulin level ( J:145076 )

increased IgG1 level ( J:145076 )

• sera levels of IgG1 are increased 3-fold over controls

• there is also a 2-fold increase in IgG1 levels following immunization with a T cell-dependent antigen as compared to controls

increased IgG2a level ( J:145076 )

• there is a 2-fold increase in IgG2a/c levels following immunization with a T cell-dependent antigen as compared to controls

increased IgG2b level ( J:145076 )

• there is a 2-fold increase in IgG2b levels following immunization with a T cell-dependent antigen as compared to controls

increased IgG2c level ( J:145076 )

• there is a 2-fold increase in IgG2a/c levels following immunization with a T cell-dependent antigen as compared to controls

increased IgG3 level ( J:145076 )

• sera levels of IgG3 are increased 3-fold over controls

increased IgM level ( J:145076 )

• there is a 2-fold increase in IgM levels following immunization with a T cell-dependent antigen as compared to controls

hematopoietic system

abnormal B cell number ( J:145076 )

decreased immature B cell number ( J:145076 )

• immature "Fraction C" pro-B cell numbers in the bone marrow are slightly decreased at 6- and 8- weeks of age

increased immature B cell number ( J:145076 )

• immature "Fraction A" pro-B cell numbers in the bone marrow are almost double that of controls at 4 weeks of age

• culturing bone marrow cells with IL-7 leads to 75% development of "Fraction C" pro-B cells compared to 50% in controls

increased transitional stage B cell number ( J:145076 )

• T1 transitional B cell numbers are increased 6-fold in the spleen of 8 week old mice

decreased mature B cell number ( J:145076 )

• the number of recirculating mature B cells in the bone marrow of 8 week old mice is about half that of controls

abnormal spleen B cell follicle morphology ( J:145076 )

• spleen follicular zone areas are slightly but significantly decreased

abnormal spleen marginal zone morphology ( J:145076 )

• marginal zones are 1.8-fold larger in area than controls

increased marginal zone B cell number ( J:145076 )

• marginal zone B cell numbers are 4-fold higher than controls

increased immunoglobulin level ( J:145076 )

increased IgG1 level ( J:145076 )

• sera levels of IgG1 are increased 3-fold over controls

• there is also a 2-fold increase in IgG1 levels following immunization with a T cell-dependent antigen as compared to controls

increased IgG2a level ( J:145076 )

• there is a 2-fold increase in IgG2a/c levels following immunization with a T cell-dependent antigen as compared to controls

increased IgG2b level ( J:145076 )

• there is a 2-fold increase in IgG2b levels following immunization with a T cell-dependent antigen as compared to controls

increased IgG2c level ( J:145076 )

• there is a 2-fold increase in IgG2a/c levels following immunization with a T cell-dependent antigen as compared to controls

increased IgG3 level ( J:145076 )

• sera levels of IgG3 are increased 3-fold over controls

increased IgM level ( J:145076 )

• there is a 2-fold increase in IgM levels following immunization with a T cell-dependent antigen as compared to controls

Genotype
MGI:3838138

ht2

• Allelic Composition: Dlk1^tm1Srba/Dlk1⁺
• Genetic Background: involves: 129X1/SvJ * C57BL/6

cellular

maternal imprinting ( J:145076 )

• locus is maternally imprinted with no gene expression detectable in neonatal liver of mice that have paternally inherited the mutant allele