Phenotypes associated with this allele

• Allele Symbol: Cebpa^tm1Dgt
• Allele Name: targeted mutation 1, Daniel G Tenen
• Allele ID: MGI:3522010
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cebpa^tm1Dgt/Cebpa^tm1Dgt	involves: 129S6/SvEvTac	MGI:3525183
cn2	Cebpa^tm1Dgt/Cebpa^tm1Dgt Tg(SFTPC-rtTA)5Jaw/0 Tg(tetO-cre)1Jaw/0	involves: 129 * 129S6/SvEvTac * C57BL/6	MGI:4942158
cn3	Cebpa^tm1Dgt/Cebpa^tm1Dgt Tg(Mx1-cre)1Cgn/0	involves: 129S6/SvEvTac * C57BL/6 * CBA	MGI:3525184

Genotype
MGI:3525183

hm1

• Allelic Composition: Cebpa^tm1Dgt/Cebpa^tm1Dgt
• Genetic Background: involves: 129S6/SvEvTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:94682 )

• no notable abnormalities in the hematopoietic system were detected

Genotype
MGI:4942158

cn2

• Allelic Composition: Cebpa^tm1Dgt/Cebpa^tm1Dgt; Tg(SFTPC-rtTA)5Jaw/0; Tg(tetO-cre)1Jaw/0
• Genetic Background: involves: 129 * 129S6/SvEvTac * C57BL/6

mortality/aging

neonatal lethality, incomplete penetrance ( J:105593 )

• although born in Mendelian ratios, most mice exposed to doxycycline from conception die of respiratory failure at birth

respiratory system

abnormal pulmonary alveolus morphology ( J:105593 )

• doxycycline-exposed newborn mice show a reduction of airspace, sometimes obliterating the alveolar structure

abnormal pulmonary alveolus epithelial cell morphology ( J:105593 )

• doxycycline-exposed newborn mice display increased numbers of Ki-67-positive alveolar cells, indicating increased alveolar cell proliferation

• in addition, neonatal alveolar cells show significantly decreased numbers of TUNEL-positive cells, indicating reduced apoptosis

absent type I pneumocytes ( J:105593 )

• doxycycline-exposed newborn mice lack type I alveolar cells

abnormal type II pneumocyte morphology ( J:105593 )

• alveoli of doxycycline-exposed newborn mice are lined by immature cuboidal type II cells with a clear cytosol, round nuclei and high glycogen content, indicating a type II cell differentiation arrest

absent alveolar lamellar bodies ( J:105593 )

• immature type II alveolar cells of doxycycline-exposed newborn mice lack lamellar bodies

absent type II pneumocytes ( J:105593 )

• doxycycline-exposed newborn mice lack mature type II alveolar cells

increased pulmonary alveolus wall thickness ( J:105593 )

• doxycycline-exposed newborn mice display thicker alveolar walls due to marked cellular accumulation

respiratory distress ( J:105593 )

• 86% of mice exposed to doxycycline from conception show signs of respiratory distress within hours after birth

respiratory failure ( J:105593 )

• most mice exposed to doxycycline from conception to birth display respiratory failure

abnormal surfactant secretion ( J:105593 )

• doxycycline-exposed newborn mice show a marked loss of surfactant protein B (SP-B) by immunostaining

• mRNAs for SP-A and SP-D are significantly down-regulated

liver/biliary system

liver/biliary system phenotype ( J:105593 )

N • doxycycline-exposed mice have a normal liver architecture and show normal Cebpa mRNA levels in liver

hematopoietic system

hematopoietic system phenotype ( J:105593 )

N • doxycycline-exposed mice exhibit normal granulocytic differentiation in fetal liver and contain granulocytes in peripheral blood

Genotype
MGI:3525184

cn3

• Allelic Composition: Cebpa^tm1Dgt/Cebpa^tm1Dgt; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CBA

mortality/aging

premature death ( J:94682 )

• mutants treated with poly I:C rarely survive beyond 4-5 weeks of age due to granulocytopenia and sepsis

hematopoietic system

abnormal myelopoiesis ( J:94682 )

• a dramatic loss of Gr-1+ Mac-1+ neutrophils/monocytes is seen in the bone marrow, spleen, and peripheral blood, however T and B cell numbers are normal

failure of myelopoiesis ( J:94682 )

• after poly I:C treatment myelocytes, neutrophils, monocytes, and eosinophils are rarely seen in the bone marrow and the number of immature myeloblasts is increased 32%

• in vitro common myeloid progenitor cells fail to form any mature granulocyte-macrophage, macrophage, or granulocytic colonies

decreased granulocyte number ( J:94682 )

• injection of poly I:C at 2 days after birth resulted in absence of mature granulocytes, however mutants do not develop anemia, thrombocytopenia, or leukemia

immune system

abnormal myelopoiesis ( J:94682 )

• a dramatic loss of Gr-1+ Mac-1+ neutrophils/monocytes is seen in the bone marrow, spleen, and peripheral blood, however T and B cell numbers are normal

failure of myelopoiesis ( J:94682 )

• after poly I:C treatment myelocytes, neutrophils, monocytes, and eosinophils are rarely seen in the bone marrow and the number of immature myeloblasts is increased 32%

• in vitro common myeloid progenitor cells fail to form any mature granulocyte-macrophage, macrophage, or granulocytic colonies

decreased granulocyte number ( J:94682 )

• injection of poly I:C at 2 days after birth resulted in absence of mature granulocytes, however mutants do not develop anemia, thrombocytopenia, or leukemia

sepsis ( J:94682 )