All Phenotypes Lat<tm4.1Mal> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lat^tm4.1Mal/Lat^tm4.1Mal	involves: 129P2/OlaHsd	MGI:3691075
cn2	Lat^tm4.1Mal/Lat^{tm6.1(HBEGF/EGFP)Mal}	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6	MGI:4357975
cx3	Lat^tm4.1Mal/Lat^tm4.1Mal Trdc^tm1Mal/Trdc^tm1Mal	involves: 129P2/OlaHsd * C57BL/6	MGI:3690995
cx4	Lat^tm4.1Mal/Lat^tm4.1Mal Tg(Lck*F505)3073Rmp/?	involves: 129P2/OlaHsd * C57BL/6 * DBA/2	MGI:4357976

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal thymus cell ratio ( J:94074 )

• thymi contain CD3+ gamma/delta TCR+ cells, in contrast to some published reports

abnormal thymocyte activation ( J:94074 )

• thymocytes are unable to be activated by anti-CD3epsilon antibody treatment

abnormal lymphocyte morphology ( J:94074 )

abnormal gamma-delta T cell differentiation ( J:94074 )

• 5.3-fold reduction in numbers found in the thymus

arrested T cell differentiation ( J:94074 )

• there is a strict developmental block in double negative T cells at the transition from the CD44-negative,CD25-positive stage to the CD44-, CD25- negative stage

decreased T cell number ( J:94074 )

• fewer gamma/delta T cells are present compared to wild-type thymi; progeny of these cells are subsequently lost before reaching periphery

decreased double-positive T cell number ( J:94074 )

• double-positive T cells are completely absent

absent CD4-positive, alpha-beta T cells ( J:94074 )

• no single-positive cells are found in the thymus

absent CD8-positive, alpha-beta T cells ( J:94074 )

• no single-positive cells are found in the thymus

absent mature gamma-delta T cells ( J:94074 )

• no gamma-delta T cells are found in the periphery

abnormal splenic cell ratio ( J:94074 )

• spleens lack gamma/delta T cells

abnormal lymph node cell ratio ( J:94074 )

• lymph nodes lack gamma/delta T cells

hematopoietic system

abnormal thymus cell ratio ( J:94074 )

• thymi contain CD3+ gamma/delta TCR+ cells, in contrast to some published reports

abnormal thymocyte activation ( J:94074 )

• thymocytes are unable to be activated by anti-CD3epsilon antibody treatment

abnormal lymphocyte morphology ( J:94074 )

abnormal gamma-delta T cell differentiation ( J:94074 )

• 5.3-fold reduction in numbers found in the thymus

arrested T cell differentiation ( J:94074 )

• there is a strict developmental block in double negative T cells at the transition from the CD44-negative,CD25-positive stage to the CD44-, CD25- negative stage

decreased T cell number ( J:94074 )

• fewer gamma/delta T cells are present compared to wild-type thymi; progeny of these cells are subsequently lost before reaching periphery

decreased double-positive T cell number ( J:94074 )

• double-positive T cells are completely absent

absent CD4-positive, alpha-beta T cells ( J:94074 )

• no single-positive cells are found in the thymus

absent CD8-positive, alpha-beta T cells ( J:94074 )

• no single-positive cells are found in the thymus

absent mature gamma-delta T cells ( J:94074 )

• no gamma-delta T cells are found in the periphery

abnormal splenic cell ratio ( J:94074 )

• spleens lack gamma/delta T cells

decreased platelet aggregation ( J:125744 )

• platelets do not aggregate in response to low doses of a snake venom clotting agonist or collagen

homeostasis/metabolism

decreased platelet aggregation ( J:125744 )

• platelets do not aggregate in response to low doses of a snake venom clotting agonist or collagen

abnormal thrombosis ( J:125744 )

• platelets are unable to form a multilayered thrombi on collagen coated surfaces

• the volume of thrombi are reduced by more than 2.6-fold and the surface area covered is reduced by 1.8-fold

endocrine/exocrine glands

abnormal thymus cell ratio ( J:94074 )

• thymi contain CD3+ gamma/delta TCR+ cells, in contrast to some published reports

abnormal thymocyte activation ( J:94074 )

• thymocytes are unable to be activated by anti-CD3epsilon antibody treatment

Allelic Composition	Lat^tm4.1Mal/Lat^{tm6.1(HBEGF/EGFP)Mal}
Genetic Background	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lat^tm4.1Mal mutation (0 available); any Lat mutation (9 available)
Lat^{tm6.1(HBEGF/EGFP)Mal} mutation (1 available); any Lat mutation (9 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal T-helper 2 physiology ( J:151840 )

• CD4⁺ T cells were first transfected with a cre recombinase vector and then treated with dipthera toxin so that surviving T cells only expressed the mutant Lat^tm1Mal allele

• when activated and transferred into T cell deficient hosts, these T cells cause splenomegaly, lymphadenopathy and secrete high levels of IL-4

• these abnormal T cells promote B cell activation in their hosts leading to high levels of IgG1 and IgE

hematopoietic system

abnormal T-helper 2 physiology ( J:151840 )

• CD4⁺ T cells were first transfected with a cre recombinase vector and then treated with dipthera toxin so that surviving T cells only expressed the mutant Lat^tm1Mal allele

• when activated and transferred into T cell deficient hosts, these T cells cause splenomegaly, lymphadenopathy and secrete high levels of IL-4

• these abnormal T cells promote B cell activation in their hosts leading to high levels of IgG1 and IgE

Allelic Composition	Lat^tm4.1Mal/Lat^tm4.1Mal Trdc^tm1Mal/Trdc^tm1Mal
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lat^tm4.1Mal mutation (0 available); any Lat mutation (9 available)
Trdc^tm1Mal mutation (1 available); any Trdc mutation (7 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal double-negative T cell morphology ( J:112658 )

• surface expression of Tcr gamma/delta heterodimers is normal, but stage 3 double negative (DN3) T cells are unable to mature beyond the EGFP^int Tcr gamma/delta^lo stage

• mature gamma/delta T cells are absent

• pool of EGFP^int Tcr gamma/delta^lo DN3 cells comprises ~0.5% of total DN3 cells

immune system

abnormal double-negative T cell morphology ( J:112658 )

• surface expression of Tcr gamma/delta heterodimers is normal, but stage 3 double negative (DN3) T cells are unable to mature beyond the EGFP^int Tcr gamma/delta^lo stage

• mature gamma/delta T cells are absent

• pool of EGFP^int Tcr gamma/delta^lo DN3 cells comprises ~0.5% of total DN3 cells

Allelic Composition	Lat^tm4.1Mal/Lat^tm4.1Mal Tg(Lck*F505)3073Rmp/?
Genetic Background	involves: 129P2/OlaHsd * C57BL/6 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lat^tm4.1Mal mutation (0 available); any Lat mutation (9 available)
Tg(Lck*F505)3073Rmp mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

spleen hyperplasia ( J:151840 )

• spleen cellularity is increased 3-fold compared to transgenics on a wild-type background

increased CD4-positive, alpha-beta T cell number ( J:151840 )

• CD4⁺CD5⁺ T cell numbers are increased over 5-fold compared to controls

increased IgE level ( J:151840 )

• serum IgE levels are about 10,000-fold higher than controls

increased IgG1 level ( J:151840 )

• serum IgG1 levels are more than 100-fold higher than controls

abnormal T-helper 2 physiology ( J:151840 )

• transgene allows rescue of deficient thymocyte development of Lat-null mice

• 60% of these T cells express IL-4 upon PMA stimulation

• Th2 T cells hyper proliferate and stimulate immunoglobulin production

increased interleukin-4 secretion ( J:151840 )

• 60% of peripheral T cells express IL-4 upon PMA stimulation

hematopoietic system

spleen hyperplasia ( J:151840 )

• spleen cellularity is increased 3-fold compared to transgenics on a wild-type background

increased CD4-positive, alpha-beta T cell number ( J:151840 )

• CD4⁺CD5⁺ T cell numbers are increased over 5-fold compared to controls

increased IgE level ( J:151840 )

• serum IgE levels are about 10,000-fold higher than controls

increased IgG1 level ( J:151840 )

• serum IgG1 levels are more than 100-fold higher than controls

abnormal T-helper 2 physiology ( J:151840 )

• transgene allows rescue of deficient thymocyte development of Lat-null mice

• 60% of these T cells express IL-4 upon PMA stimulation

• Th2 T cells hyper proliferate and stimulate immunoglobulin production

growth/size/body

spleen hyperplasia ( J:151840 )

• spleen cellularity is increased 3-fold compared to transgenics on a wild-type background

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/23/2024 MGI 6.23