Phenotypes associated with this allele

• Allele Symbol: Tg(CAG-EGFP)D4Nagy
• Allele Name: transgene insertion D4, Andras Nagy
• Allele ID: MGI:3055027
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Porcn^tm1.1Jrt/Porcn^+ Tg(CAG-EGFP)D4Nagy/0 Tg(Zp3-cre)3Mrt/0	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6J * C57BL/6NCr * FVB/N	MGI:5523431
cn2	Porcn^tm1.1Jrt/Y Tg(CAG-EGFP)D4Nagy/0 Tg(Zp3-cre)3Mrt/0	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6J * C57BL/6NCr * FVB/N	MGI:5523432
cn3	Hccs^tm1Tcc/Hccs^+ Nkx2-5^tm2(cre)Rph/Nkx2-5^+ Tg(CAG-EGFP)D4Nagy/0 Tg(Hmgcr-lacZ)H253Sest/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2	MGI:3826982
cx4	Tg(CAG-EGFP)D4Nagy/0 Xist^tm5.2Nbd/Xist^+	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ	MGI:4950463

Genotype
MGI:5523431

cn1

• Allelic Composition: Porcn^tm1.1Jrt/Porcn⁺; Tg(CAG-EGFP)D4Nagy/0; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6J * C57BL/6NCr * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

embryo

embryo phenotype ( J:198636 )

♀N • heterozygous females implant normally when the mutant allele ant the cre transgene are from the mother and the reporter is from the father

Genotype
MGI:5523432

cn2

• Allelic Composition: Porcn^tm1.1Jrt/Y; Tg(CAG-EGFP)D4Nagy/0; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6J * C57BL/6NCr * FVB/N

embryo

failure to gastrulate ( J:198636 )

♂ • hemizygous males fail to gastrulate when mutant and cre are derived from the mother and the reporter is derived from the father

Genotype
MGI:3826982

cn3

• Allelic Composition: Hccs^tm1Tcc/Hccs⁺; Nkx2-5^tm2(cre)Rph/Nkx2-5⁺; Tg(CAG-EGFP)D4Nagy/0; Tg(Hmgcr-lacZ)H253Sest/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2

cardiovascular system

abnormal fetal cardiomyocyte morphology ( J:143285 )

♀ • at E12.5, the relative volume of abnormal heart tissue begins to decline to 42% in ventricles and 47% in atria and is further reduced at E16.5 to 18% in ventricles and 19% in the atria then 10% in ventricles and 17% in the atria prior to birth

♀ • at E12.5 and E13.5, cardiomyocytes appear small and round compared to wild-type cells

♀ • however, the absolute volume of heart tissue before birth is unchanged

decreased fetal cardiomyocyte size ( J:143285 )

♀ • cardiomyocytes appear smaller at E12.5 and E13.5

abnormal fetal cardiomyocyte proliferation ( J:143285 )

♀ • while the number of affected cardiomyocytes decreases proliferation of normal cardiomyocytes increases in a compensatory manner

muscle

abnormal fetal cardiomyocyte proliferation ( J:143285 )

♀ • while the number of affected cardiomyocytes decreases proliferation of normal cardiomyocytes increases in a compensatory manner

cellular

abnormal fetal cardiomyocyte proliferation ( J:143285 )

♀ • while the number of affected cardiomyocytes decreases proliferation of normal cardiomyocytes increases in a compensatory manner

Genotype
MGI:4950463

cx4

• Allelic Composition: Tg(CAG-EGFP)D4Nagy/0; Xist^tm5.2Nbd/Xist⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ

cellular

abnormal dosage compensation, by inactivation of X chromosome ( J:171502 )

♀ • when the allele is maternally inherited, mice exhibit secondary non-random X inactivation compared with wild-type mice