All Phenotypes Ubr5<tm1Ckww> MGI Mouse

embryonic lethality during organogenesis, complete penetrance ( J:92269 )

• embryos are present at E10.5 but only partially resorbed embryos are found at E11.5

increased embryonic tissue cell apoptosis ( J:92269 )

• increased apoptosis is seen throughout the embryo at E9.5 and E10.5

small pharyngeal arch ( J:92269 )

• the branchial arches are underdeveloped

incomplete embryo turning ( J:92269 )

• turning is absent in greater than 90% of embryos at E9.5 and E10.5

embryonic growth retardation ( J:92269 )

• at E8.5 homozygous embryos are developmentally retarded by about 0.5 days and this becomes more severe by E9.5 and E10.5 with significant underdevelopment of the head and branchial arches

decreased embryo size ( J:92269 )

abnormal allantois morphology ( J:92269 )

• at E8.5 the allantois fails to expand and remains narrow and uneven without the numerous vessels seen in wild-type mice

• only isolated islands of endothelial cells that fail to coalesce in to blood vessels are seen in the allantois

• at E10.5 the allantois forms a large blood filled mass near the hindgut

abnormal visceral yolk sac morphology ( J:92269 )

• the visceral endoderm and mesoderm are more widely separated with few connections

pale yolk sac ( J:92269 )

• the yolk sac is pale and fragile

failure of chorioallantoic fusion ( J:92269 )

• at E8.5 failure of chorioallantoic fusion is seen in greater than 90% of embryos and in those where fusion occurs attachments are weak and placentation is not observed

abnormal vitelline vascular remodeling ( J:92269 )

• at E9.5 no organized vessel formation is seen and at E10.5 vessels are smaller and disorganized with many dead-end sprouts

increased embryonic tissue cell apoptosis ( J:92269 )

• increased apoptosis is seen throughout the embryo at E9.5 and E10.5

decreased cell proliferation ( J:92269 )

• decreased cell proliferation is seen throughout the embryo at E9.5 and by E10.5 almost no proliferation is seen

abnormal angiogenesis ( J:92269 )

• angiogenesis is impaired in the yolk sac and allantois; however early development of the dorsal aorta appears normal

pericardial effusion ( J:92269 )

• pericardial effusion is seen at E10.5

abnormal brain development ( J:92269 )

• far less neural epithelium is present and the epithelial structure is disorganized compared to wild-type embryos