Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Unc13atm2Bros mutation
(0 available);
any
Unc13a mutation
(74 available)
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mortality/aging
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• despite initially breathing, responding to tactile stimuli and exhibiting pain-avoidance reflexes, mice die within 1 to 2 hours of birth
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nervous system
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• probability of vesicle release is increased (10.5%+/-0.8% compared to 6.9%+/-0.6% in wild-type cells)
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• ready releasable pools (RRPs) of vesicles are reduced
• however, the rate of RRP refill after complete depletion is normal
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• after a 40Hz train, the recovery response to RRP depletion is slower than in wild-type cells
• after a 10Hz continuous stimulation in a hypertonic solution and return to normal osmolarity, the recovery response to RRP depletion is slower than in wild-type cells
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• while excitatory postsynaptic currents are normal they only exhibit a small response to treatment with (4beta-phorbol-12,13-dibutyrate) PDBu
• at 10Hz action potential trains, excitatory postsynaptic current depression is enhanced relative to that in wild-type cells
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Unc13atm2Bros mutation
(0 available);
any
Unc13a mutation
(74 available)
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mortality/aging
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• mice die within 1 to 2 hours of birth
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Allelic
Composition |
Unc13atm2Bros/Unc13a+
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Genetic
Background |
Not Specified |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Unc13atm2Bros mutation
(0 available);
any
Unc13a mutation
(74 available)
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homeostasis/metabolism
endocrine/exocrine glands
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• mice exhibit a reduction in the size of ready releasable pools (RRPs) of vesicles in islet cells and do not respond to PMA (12-myristate 13-acetate ester) treatment
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Unc13atm2Bros mutation
(0 available);
any
Unc13a mutation
(74 available)
Unc13btm2Bros mutation
(1 available);
any
Unc13b mutation
(119 available)
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nervous system
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• spontaneous release rates are intermediate and are increased by PDBu (4beta-phorbol-12,13-dibutyrate) treatment
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• the number of fusion-competent vesicles is reduced to 2763+/-305 vesicles compared to 4109+/-722 vesicles in wild-type mice
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• at 10Hz action potential trains, excitatory postsynaptic current depression is enhanced relative to that in wild-type cells but not as much as in homozygotes and further enhanced by treatment with PDBu (4beta-phorbol-12,13-dibutyrate)
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Unc13atm2Bros mutation
(0 available);
any
Unc13a mutation
(74 available)
Unc13btm2Bros mutation
(1 available);
any
Unc13b mutation
(119 available)
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nervous system
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• the rate of vesicle release is doubled compared to in wild-type cells for all doses tested
• spontaneous release rates are increased and are not effected by PDBu (4beta-phorbol-12,13-dibutyrate) treatment
• the probability of vesicle release is increased relative to in wild-type cells
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• the number of fusion-competent vesicles is reduced to 2299+/-300 vesicles compared to 4109+/-722 vesicles in wild-type mice
• following stimulation with 250mM sucrose, the number of vesicles released is greater (2000+/-460 vesicles) than in wild-type cells (1100+/-300 vesicles)
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• at 10Hz action potential trains, excitatory postsynaptic current depression is enhanced relative to that in wild-type cells and is further enhanced by treatment with PDBu by (4beta-phorbol-12,13-dibutyrate)
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• the absolute frequency of miniature excitory postsynaptic currents is more than doubled that in wild-type cells but similar to that in phorbol ester-treated wild-type cells
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• long term potential is enhanced relative to in wild-type cells but similar to in that in wild-type cells treated with PDBu (4beta-phorbol-12,13-dibutyrate)
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Analysis Tools