Unc13b^tm2Bros
Targeted Allele Detail

Summary

• Symbol: Unc13b^tm2Bros
• Name: unc-13 homolog B; targeted mutation 2, Nils Brose
• MGI ID: MGI:2449706
• Synonyms: Munc13-2 KO, Unc13b^KO, Unc13b^tm1Rmnd
• Gene: Unc13b Location: Chr4:43058953-43264871 bp, + strand Genetic Position: Chr4, 23.0 cM, cytoband B1
• Alliance: Unc13b^tm2Bros page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77339
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of nucleotides 1439-2436 with a neomycin resistance gene via homologous recombination. Gene expression was completely absent in homozygous mutant animals as determined by Western blot analysis. An antibody recognizing all proteins isoforms was used for Western blotting. (J:77339)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Unc13b Mutation: 120 strains or lines available

References

• Original: J:77339 Varoqueaux F, et al., Total arrest of spontaneous and evoked synaptic transmission but normal synaptogenesis in the absence of Munc13-mediated vesicle priming. Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):9037-42
• All: 40 reference(s)