Phenotypes associated with this allele

• Allele Symbol: Syngap1⁺
• Allele Name: wild type
• Allele ID: MGI:3049765
• Summary: 9 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Syngap1^tm1Grnt/Syngap1^+	involves: 129P2/OlaHsd * MF1	MGI:3581300
ht2	Syngap1^tm1Rlh/Syngap1^+	involves: 129S1/Sv * 129X1/SvJ	MGI:5469876
ht3	Syngap1^tm1Rlh/Syngap1^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3576467
ht4	Syngap1^tm1Rlh/Syngap1^+	involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:5485349
cn5	Gt(ROSA)26Sor^tm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor^+ Syngap1^tm1.1Geno/Syngap1^+	involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6 * C57BL/6NCr	MGI:5469877
cn6	Syngap1^tm2Geno/Syngap1^+ Tg(CAG-cre/Esr1*)5Amc/0	involves: 129S2/SvPas * C57BL/6 * CBA	MGI:5469879
cx7	Dlg4^tm1Grnt/Dlg4^tm1Grnt Syngap1^tm1Grnt/Syngap1^+	involves: 129P2/OlaHsd * MF1	MGI:3581308
cx8	Hras^tm1Grnt/Hras^tm1Grnt Syngap1^tm1Grnt/Syngap1^+	involves: 129P2/OlaHsd * MF1	MGI:3581305
cx9	Syngap1^tm2Geno/Syngap1^+ Tg(CAG-cre/Esr1*)5Amc/0	involves: 129S2/SvPas * C57BL/6 * CBA	MGI:5469878

Genotype
MGI:3581300

ht1

• Allelic Composition: Syngap1^tm1Grnt/Syngap1⁺
• Genetic Background: involves: 129P2/OlaHsd * MF1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

impaired synaptic plasticity ( J:98903 )

reduced long-term potentiation ( J:98903 )

• reduced long term potentiation in the hippocampal CA1 region (across a wide range of LTP induction protocols) in the absence of any detectable alteration in basal synaptic transmission and NMDAR-mediated synaptic currents

behavior/neurological

abnormal spatial learning ( J:98903 )

• heterozygotes initially learn slower than wildtype in spatial water maze tests, however with further training, exhibit normal learning indicating unimpaired long-term memory

Genotype
MGI:5469876

ht2

• Allelic Composition: Syngap1^tm1Rlh/Syngap1⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

nervous system

nervous system phenotype ( J:193208 )

N • at P14, mice exhibit normal medial perforant path release probability and intrinsic spiking of dentate gyrus neurons

increased susceptibility to pharmacologically induced seizures ( J:193208 )

• fluorothyl-induced

audiogenic seizures ( J:193208 )

abnormal dendrite morphology ( J:193208 )

• at P14, dentate gyrus neuron dendritic trees exhibit a decrease in the spatial volume compared with in wild-type mice

abnormal dendritic spine morphology ( J:193208 )

• larger spines by P14 and persisting into adulthood

• more mushroom-type spines and fewer stubby spines beginning in the second postnatal week

• spines are less motile at P14

• some spines exhibit a spontaneous increase in head volume

• however, spine density is normal

abnormal CNS synaptic transmission ( J:193208 )

• photosimulation-evoked signals originating in the dentate gyrus are amplified as they spread through the hippocampus unlike in wild-type mice

• synaptic transmission in the Schaeffer Collateral pathway in the CA1 is strong during early development compared with wild-type mice

• however, synaptic transmission in the Schaeffer Collateral pathway in the CA1 in adult mice is normal

abnormal excitatory postsynaptic potential ( J:193208 )

• by P14, field excitatory postsynaptic potential is increased compared to in control mice

• however, synaptic function at P9, P21 and later is normal

abnormal glutamate-mediated receptor currents ( J:193208 )

• at P14, mice exhibit an increase in the ratio of AMPA/NMDA currents in dentate gyrus granule neurons compared with wild-type mice

• however, NMDAR-evoked synaptic transmission is normal

enhanced AMPA-mediated synaptic currents ( J:193208 )

• increased sensitivity at P14

abnormal miniature excitatory postsynaptic currents ( J:193208 )

• increased amplitude and frequency at P14

• however, both are normal at P21

abnormal miniature inhibitory postsynaptic currents ( J:193208 )

• increased frequency and amplitude in the dentate gyrus neurons

increased miniature inhibitory postsynaptic current amplitude ( J:193208 )

• in the dentate gyrus neurons

increased miniature inhibitory postsynaptic current frequency ( J:193208 )

• in the dentate gyrus neurons

behavior/neurological

behavior/neurological phenotype ( J:193208 )

N • mice exhibit normal contextual fear memory

increased susceptibility to pharmacologically induced seizures ( J:193208 )

• fluorothyl-induced

abnormal discrimination learning ( J:193208 )

• mice are unable to discriminate between two contexts during contextual fear training unlike wild-type mice

hyperactivity ( J:193208 )

• in an open field at P14

audiogenic seizures ( J:193208 )

Genotype
MGI:3576467

ht3

• Allelic Composition: Syngap1^tm1Rlh/Syngap1⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

nervous system

reduced long-term potentiation ( J:97391 )

• long term potentiation induced by TBS was significantly decreased in the CA1 region of hippocampal slices, however there was no difference in the magnitude of long term depression

Genotype
MGI:5485349

ht4

• Allelic Composition: Syngap1^tm1Rlh/Syngap1⁺
• Genetic Background: involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6J

behavior/neurological

impaired cued conditioning behavior ( J:194146 )

• mutants exhibit reduced freezing in response to a conditioning tone previously associated with a mild footshock, however the freezing response is intact as behavioral responses to a fear-associated context are normal

• activity during conditioning tone presentation is normal, however activity is elevated during tone presentation in the retrieval test

abnormal spatial working memory ( J:194146 )

• although mutants are able to learn forced alteration rule in the automated eight-arm radial maze, mutants show a time-dependent decrease in nonmatching performance, indicating spatial working memory deficits

decreased anxiety-related response ( J:194146 )

• mutants exhibit random vectors through the open field unlike wild-type mice that primarily run around the outside chamber and spend most of the time in the corners, indicating abnormal anxiety levels

• mutants are more likely to venture into the center of the field during the first few minutes of the test session, indicating decreased thigmotaxis

• in the elevated plus maze, mutants spend less time in the closed arm and more time in the open arm compared to wild-type mice, indicating decreased anxiety levels

increased startle reflex ( J:194146 )

• mutants respond more strongly to the 120 dB stimulus then wild-type mice

• mutants show enhanced startle responses at the highest amplitudes (loudest pulses)

increased locomotor activity ( J:194146 )

• mutants exhibit elevated horizontal activity compared to wild-type littermates and this behavior is nonhabituating over repeated trials

• juvenile, newly weaned mutants also exhibit increased horizontal activity

• antipsychotic drug clozapine ameliorates the persistent hyperactivity

• the NMDAR antagonist, MK-801, has a reduced effect on hyperactivity in mutants compared to wild-type mice

increased horizontal stereotypic behavior ( J:194146 )

• mutants show an increase in stereotypies in the open field test compared to wild-type littermates, most pronounced at the beginning of a 2 hour test session

• mutants show increased stereotypic behavior in 30 minute open field trials over 5 consecutive days, indicating behavior is nonhabituating

abnormal social/conspecific interaction behavior ( J:194146 )

• mutants are unable to distinguish between familiar and novel conspecifics compared to wild-type mice which spend more time with the novel conspecifics

social withdrawal ( J:194146 )

• in a test for social isolation, mutants spend more time in the center compartment (a space that is separated from the other mice) than in ends of chamber where novel conspecifics were placed, indicating a preference for social isolation

nervous system

decreased prepulse inhibition ( J:194146 )

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
schizophrenia		DOID:5419	OMIM:181500	J:194146

Genotype
MGI:5469877

cn5

• Allelic Composition: Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺; Syngap1^tm1.1Geno/Syngap1⁺
• Genetic Background: involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6 * C57BL/6NCr

nervous system

abnormal CNS synaptic transmission ( J:193208 )

• adult injection of cre-expressing virus produces an increase in intrinsic excitability of dentate gyrus neurons compared with control mice

abnormal glutamate-mediated receptor currents ( J:193208 )

• mice injected with a cre-expressing virus exhibit an increase in dentate gyrus neuron AMPA/NMDA ratio compared with control mice

Genotype
MGI:5469879

cn6

• Allelic Composition: Syngap1^tm2Geno/Syngap1⁺; Tg(CAG-cre/Esr1*)5Amc/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * CBA

behavior/neurological

abnormal behavior ( J:193208 )

• mice exhibit behavioral abnormalities as in Syngap1^tm1Rlh heterozygotes

abnormal spatial working memory ( J:193208 )

• untreated mice and mice treated with tamoxifen in adulthood exhibit absence of spontaneous alteration in a T-maze compared with control mice

decreased anxiety-related response ( J:193208 )

• untreated mice and mice treated with tamoxifen in adulthood spend increased time in open arms of an elevated plus maze compared with control mice

hyperactivity ( J:193208 )

• untreated mice and mice treated with tamoxifen in adulthood exhibit increased activity in an open field compared with control mice

Genotype
MGI:3581308

cx7

• Allelic Composition: Dlg4^tm1Grnt/Dlg4^tm1Grnt; Syngap1^tm1Grnt/Syngap1⁺
• Genetic Background: involves: 129P2/OlaHsd * MF1

nervous system

enhanced long-term potentiation ( J:98903 )

• 150 plulse trains of 5 Hz stimulations induced a more than twofold increase in long term potentiation (LTP) than in wildtype, which was similar to the LTP seen in single homozygous Dlgh4 mice

Genotype
MGI:3581305

cx8

• Allelic Composition: Hras^tm1Grnt/Hras^tm1Grnt; Syngap1^tm1Grnt/Syngap1⁺
• Genetic Background: involves: 129P2/OlaHsd * MF1

nervous system

reduced long-term potentiation ( J:98903 )

• long term potentiation induced by 150 pulses of 5 Hz stimulation in the hippocampal CA1 region was reduced compared to wildtype and single homozygous Hras1 mutants

Genotype
MGI:5469878

cx9

• Allelic Composition: Syngap1^tm2Geno/Syngap1⁺; Tg(CAG-cre/Esr1*)5Amc/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * CBA

behavior/neurological

abnormal behavior ( J:193208 )

• mice exhibit behavioral abnormalities as in Syngap1^tm1Rlh heterozygotes

abnormal spatial working memory ( J:193208 )

• untreated mice and mice treated with tamoxifen in adulthood exhibit absence of spontaneous alteration in a T-maze compared with control mice

decreased anxiety-related response ( J:193208 )

• untreated mice and mice treated with tamoxifen in adulthood spend increased time in open arms of an elevated plus maze compared with control mice

hyperactivity ( J:193208 )

• untreated mice and mice treated with tamoxifen in adulthood exhibit increased activity in an open field compared with control mice