Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek1tm1Jmr mutation
(1 available);
any
Chek1 mutation
(41 available)
Tg(Wap-cre)11738Mam mutation
(3 available)
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cellular
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• about 10% - 12% is seen in the differentiating lobuloalveolar mammary epithelial cells compared to less than 1% in wild-type
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• about 4% of cells in the mammary glands are proliferating at day 1 of lactation compared to 6.5% in wild-type
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endocrine/exocrine glands
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• the number of lobuloalveolar mammary epithelial cells is reduced by 50% to 60% at day 1 of lactation in proportion to the number of cells expressing cre
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• females fail to nurse their young
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reproductive system
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• the number of lobuloalveolar mammary epithelial cells is reduced by 50% to 60% at day 1 of lactation in proportion to the number of cells expressing cre
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integument
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• the number of lobuloalveolar mammary epithelial cells is reduced by 50% to 60% at day 1 of lactation in proportion to the number of cells expressing cre
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• females fail to nurse their young
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek1tm1Jmr mutation
(1 available);
any
Chek1 mutation
(41 available)
Tg(Wap-cre)11738Mam mutation
(3 available)
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cellular
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• about 35% of cells in the mammary glands are proliferating at day 1 of lactation compared to 6.5% in wild-type
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embryo
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• significantly fewer beta-gal+ melanoblasts are noted in the head and trunk regions at E12.5, and by E13.5, melanoblasts are completely lost even from the pinna, vibrissae, and around the eyes
• many cleaved caspase 3+ melanoblasts are observed in the head and trunk regions, unlike in control embryos, suggesting increased melanoblast apoptosis
• however, the number and distribution of beta-gal+ melanoblasts is normal at E10.5 and E11.5
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nervous system
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• significantly fewer beta-gal+ melanoblasts are noted in the head and trunk regions at E12.5, and by E13.5, melanoblasts are completely lost even from the pinna, vibrissae, and around the eyes
• many cleaved caspase 3+ melanoblasts are observed in the head and trunk regions, unlike in control embryos, suggesting increased melanoblast apoptosis
• however, the number and distribution of beta-gal+ melanoblasts is normal at E10.5 and E11.5
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cellular
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• many gamma-H2AX+ melanoblasts are noted in the head and trunk regions, unlike in control embryos, suggesting that aberrant DNA replication leads to spontaneous DNA damage and ultimately cell death
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homeostasis/metabolism
pigmentation
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• despite a slight reduction of melanoblast numbers at E13.5, adult mice exhibit normal coat pigmentation relative to control mice
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embryo
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• at E13.5, a slight but significant reduction of melanoblast numbers is noted in the head, dorsal, and ventral regions relative to control embryos
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nervous system
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• at E13.5, a slight but significant reduction of melanoblast numbers is noted in the head, dorsal, and ventral regions relative to control embryos
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek1tm1Jmr mutation
(1 available);
any
Chek1 mutation
(41 available)
Tg(Tyr-cre)1Lru mutation
(1 available)
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pigmentation
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• no DCT-expressing melanocytes are observed in the hair follicles, unlike in control mice
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• adult male mice are completely devoid of coat pigmentation, unlike control mice
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• male mice show complete lack of epidermal melanocytes
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• adult males show a marked reduction or absence of neural crest-derived melanocytes in the eyes, unlike control mice
• however, males retain normal eye pigmentation in the retinal pigmented epithelium (RPE) due to inefficient transgene expression in the RPE
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integument
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• no DCT-expressing melanocytes are observed in the hair follicles, unlike in control mice
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• adult male mice are completely devoid of coat pigmentation, unlike control mice
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• male mice show complete lack of epidermal melanocytes
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Analysis Tools