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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Ptpn22tm2Achn
targeted mutation 2, Andrew C Chan
MGI:3043477
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Ptpn22tm2Achn/Ptpn22tm2Achn B6.Cg-Ptpn22tm2Achn MGI:3842292
hm2
Ptpn22tm2Achn/Ptpn22tm2Achn involves: C57BL/6 MGI:3043479
cx3
Ptpn22tm2Achn/Ptpn22tm2Achn
Ptprctm1Weis/Ptprctm1Weis
B6.Cg-Ptprctm1Weis Ptpn22tm2Achn MGI:3842290


Genotype
MGI:3842292
hm1
Allelic
Composition
Ptpn22tm2Achn/Ptpn22tm2Achn
Genetic
Background
B6.Cg-Ptpn22tm2Achn
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptpn22tm2Achn mutation (1 available); any Ptpn22 mutation (36 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• double-positive thymocytes have CD5 and CD69 slighlty upregulated and are more responsive to in vitro stimulation
• the CD4:CD8 T cell ratio in the periphery is over 1.5 at six months of age
• CD44hi CD62Llo memory T cells are increased about 2-fold at 6 months of age compared to controls
• differences are noticeable at 3 months of age with increases progressing with age
• the activation marker CD69 is slightly upregulated on both CD4 and CD8 T cells in vivo
• nave CD8 T cells are hyper-response to in vitro stimulation
• double-positive thymocytes are more responsive to in vitro stimulation than controls
• mild lymphadenopathy is observed in these mice

hematopoietic system
• double-positive thymocytes have CD5 and CD69 slighlty upregulated and are more responsive to in vitro stimulation
• the CD4:CD8 T cell ratio in the periphery is over 1.5 at six months of age
• CD44hi CD62Llo memory T cells are increased about 2-fold at 6 months of age compared to controls
• differences are noticeable at 3 months of age with increases progressing with age
• the activation marker CD69 is slightly upregulated on both CD4 and CD8 T cells in vivo
• nave CD8 T cells are hyper-response to in vitro stimulation
• double-positive thymocytes are more responsive to in vitro stimulation than controls

endocrine/exocrine glands
• double-positive thymocytes are more responsive to in vitro stimulation than controls




Genotype
MGI:3043479
hm2
Allelic
Composition
Ptpn22tm2Achn/Ptpn22tm2Achn
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptpn22tm2Achn mutation (1 available); any Ptpn22 mutation (36 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• homozygous mice older than 6 months develop enlarged spleens

hematopoietic system
• homozygous mice older than 6 months develop enlarged spleens
• associated with the increased germinal center formation is an increase in GL-7+ B cells and CD23+ follicular B cells
• an increase in CD23+ follicular B cells is seen
• homozygous mice display increased numbers of large, well-formed germinal centers compared to wild-type mice
• no evidence of autoimmune-mediated organ damage is seen despite the spontaneous formation off germinal centers
• serum levels of IgE are increased in homozygotes compared to wild-type mice
• associated with the increased germinal center formation is an increase IgG1+ foci
• serum levels of IgG1 are increased in homozygotes compared to wild-type mice
• serum levels of IgG2a are increased in homozygotes compared to wild-type mice
• following antigen stimulation increased development of CD4+ and CD8+ T cells is seen in homozygotes
• 3 - 4 days after T cell receptor activation CD4+ and CD8+ T cells display increased cell cycling, enhanced proliferation, and increased cytokine production compared to T cells from wild-type mice
• negative selection of T cells is unaffected in homozygous mice

immune system
• homozygous mice older than 6 months develop enlarged spleens
• associated with the increased germinal center formation is an increase in GL-7+ B cells and CD23+ follicular B cells
• an increase in CD23+ follicular B cells is seen
• homozygous mice display increased numbers of large, well-formed germinal centers compared to wild-type mice
• no evidence of autoimmune-mediated organ damage is seen despite the spontaneous formation off germinal centers
• serum levels of IgE are increased in homozygotes compared to wild-type mice
• associated with the increased germinal center formation is an increase IgG1+ foci
• serum levels of IgG1 are increased in homozygotes compared to wild-type mice
• serum levels of IgG2a are increased in homozygotes compared to wild-type mice
• following antigen stimulation increased development of CD4+ and CD8+ T cells is seen in homozygotes
• 3 - 4 days after T cell receptor activation CD4+ and CD8+ T cells display increased cell cycling, enhanced proliferation, and increased cytokine production compared to T cells from wild-type mice
• negative selection of T cells is unaffected in homozygous mice
• homozygous mice display increased numbers of large, well-formed germinal centers compared to wild-type mice
• no evidence of autoimmune-mediated organ damage is seen despite the spontaneous formation off germinal centers
• homozygous mice older than 6 months develop enlarged lymph nodes




Genotype
MGI:3842290
cx3
Allelic
Composition
Ptpn22tm2Achn/Ptpn22tm2Achn
Ptprctm1Weis/Ptprctm1Weis
Genetic
Background
B6.Cg-Ptprctm1Weis Ptpn22tm2Achn
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptpn22tm2Achn mutation (1 available); any Ptpn22 mutation (36 available)
Ptprctm1Weis mutation (3 available); any Ptprc mutation (145 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• 33% of mice die between 10 and 12 months of age
• likely cause of death is glomerulonephritis

growth/size/body
• decreased body weight is observed in mice starting at 6 months of age
• mice that survive to 12 months of age are smaller than controls

immune system
• double-positive thymocytes have CD5 and CD69 upregulated and are hyper-responsive to in vitro stimulation
• T1 and T2 B cell numbers are increased in the spleen similar to mice homozygous for just the Ptprctm1Weis allele
• follicular B cell numbers are reduced by about a third compared to controls
• a similar phenotype is observed in mice homozygous for just the Ptprctm1Weis allele
• plasma cell numbers in the spleen is increased 4-fold at 3 months of age compared to controls
• a similar phenotype is observed in mice homozygous for just the Ptprctm1Weis allele
• the CD4:CD8 T cell ratio in the periphery is over 2.5 at six months of age
• shift in ratio is evident at six weeks of age and lasts through 12 months of age
• CD44hi CD62Llo memory T cells are increased about 3-fold at 6 months of age compared to controls
• differences are noticeable at 2 months of age with increases progressing with age
• an increased percentage of B cells express the activation marker CD69 in vivo at 6 months of age
• a larger percentage of B cells express CD69 than controls in response to stimulation in vitro
• follicular B cells are hyper-responsive to stimulation in vitro
• a similar phenotype is observed in mice homozygous for just the Ptprctm1Weis allele
• the activation marker CD69 is upregulated on both CD4 and CD8 T cells in vivo
• double-positive thymocytes are hyper-responsive to in vitro stimulation
• lymphadenopathy is observed as early as 8 weeks of age
• is progressive with time and greatly exceeds the mild lymphoproliferation evident in each of the single mutants
• cell counts are variable with some counts being 10-fold higher than controls at six months of age
• lymphadenopathy is generally out of proportion to the degree of splenomegaly observed
• auto-IgG antibodies are detected in some mice starting at 3 months of age
• by 9 months of age, auto-antibodies titers can reach levels observed in MRL/Lpr mice
• perivascular infiltrates occur in the liver of mice over 12 months of age
• kidneys of 12 month old mice have perivascular lymphocytic infiltrates, interstitial lymphocytic infiltrates, and hypercellular glomeruli with evidence of segmental sclerosis
• perivascular infiltrates occur in the lung of mice over 12 months of age

renal/urinary system
• mice over 12 months of age have variable degrees of proteinuria
• kidneys of 12 month old mice have perivascular lymphocytic infiltrates, interstitial lymphocytic infiltrates, and hypercellular glomeruli with evidence of segmental sclerosis
• kidneys of 12 month old mice have hypercellular glomeruli with evidence of segmental sclerosis
• kidneys from mice over 12 months of age are pale and nodular

homeostasis/metabolism
• mice over 12 months of age have variable degrees of proteinuria

liver/biliary system
• perivascular infiltrates occur in the liver of mice over 12 months of age

respiratory system
• perivascular infiltrates occur in the lung of mice over 12 months of age

hematopoietic system
• double-positive thymocytes have CD5 and CD69 upregulated and are hyper-responsive to in vitro stimulation
• T1 and T2 B cell numbers are increased in the spleen similar to mice homozygous for just the Ptprctm1Weis allele
• follicular B cell numbers are reduced by about a third compared to controls
• a similar phenotype is observed in mice homozygous for just the Ptprctm1Weis allele
• plasma cell numbers in the spleen is increased 4-fold at 3 months of age compared to controls
• a similar phenotype is observed in mice homozygous for just the Ptprctm1Weis allele
• the CD4:CD8 T cell ratio in the periphery is over 2.5 at six months of age
• shift in ratio is evident at six weeks of age and lasts through 12 months of age
• CD44hi CD62Llo memory T cells are increased about 3-fold at 6 months of age compared to controls
• differences are noticeable at 2 months of age with increases progressing with age
• an increased percentage of B cells express the activation marker CD69 in vivo at 6 months of age
• a larger percentage of B cells express CD69 than controls in response to stimulation in vitro
• follicular B cells are hyper-responsive to stimulation in vitro
• a similar phenotype is observed in mice homozygous for just the Ptprctm1Weis allele
• the activation marker CD69 is upregulated on both CD4 and CD8 T cells in vivo
• double-positive thymocytes are hyper-responsive to in vitro stimulation

endocrine/exocrine glands
• double-positive thymocytes are hyper-responsive to in vitro stimulation





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last database update
01/24/2023
MGI 6.22
The Jackson Laboratory