Phenotypes associated with this allele

• Allele Symbol: Retn^tm1Laz
• Allele Name: targeted mutation 1, Mitchell A Lazar
• Allele ID: MGI:3043419
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Retn^tm1Laz/Retn^tm1Laz	involves: 129S/SvEv * C57BL/6	MGI:3043421
cx2	Retn^tm1Laz/Retn^tm1Laz Tg(CD68-RETN)#Laz/?	involves: 129S6/SvEvTac * C57BL/6	MGI:5002455

Genotype
MGI:3043421

hm1

• Allelic Composition: Retn^tm1Laz/Retn^tm1Laz
• Genetic Background: involves: 129S/SvEv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

adipose tissue phenotype ( J:90033 )

N • the size and distribution of adipose depots is normal in homozygotes

homeostasis/metabolism

decreased circulating glucose level ( J:90033 )

• blood glucose levels after 4 ? 6 hours of or overnight fasting are significantly lower homozygotes compared to weight matched wild-type mice

• during constant hyperinsulinemia homozygotes require more glucose to maintain a constant glucose level primarily because of decreased glucose production

• administration of pyruvate does not increase blood glucose levels in homozygotes as much as in wild-type mice suggesting impaired gluconeogenesis

improved glucose tolerance ( J:90033 )

• on a high-fat diet glucose tolerance is significantly better in homozygotes compared to wild-type mice

decreased liver triglyceride level ( J:90033 )

• hepatic triglyceride levels tend to be lower in homozygotes

liver/biliary system

decreased liver triglyceride level ( J:90033 )

• hepatic triglyceride levels tend to be lower in homozygotes

Genotype
MGI:5002455

cx2

• Allelic Composition: Retn^tm1Laz/Retn^tm1Laz; Tg(CD68-RETN)#Laz/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

immune system

white adipose tissue inflammation ( J:171262 )

• mice exhibit an increase in inflammatory markers (Tnfa, Il6, Il1b, Cxcl15, Cd68) and an accumulation of macrophages in WAT

muscle

increased skeletal muscle triglyceride level ( J:171262 )

• mice exhibit a 52% increase in muscle triglyceride levels on a high fat diet

adipose tissue

white adipose tissue inflammation ( J:171262 )

• mice exhibit an increase in inflammatory markers (Tnfa, Il6, Il1b, Cxcl15, Cd68) and an accumulation of macrophages in WAT

homeostasis/metabolism

increased circulating insulin level ( J:171262 )

• after 2-3 weeks on a high fat (60%) diet, male mice exhibit increased serum insulin, but not glucose, levels

increased circulating free fatty acids level ( J:171262 )

• mice on a high fat diet exhibit significantly increased free fatty acid levels

increased circulating glycerol level ( J:171262 )

• mice on a high fat diet exhibit significantly increased glycerol levels

impaired glucose tolerance ( J:171262 )

• on a high fat diet

insulin resistance ( J:171262 )

• glucose-infusion rate is 39% lower than controls

• decrease in insulin sensitivity as determined by insulin tolerance tests

• decreased glucose disposal rate

• insulin stimulated glucose uptake in skeletal muscle and white adipose tissue is decreased (respectively 56% and 22%) compared to Retn^-/- controls

increased skeletal muscle triglyceride level ( J:171262 )

• mice exhibit a 52% increase in muscle triglyceride levels on a high fat diet

enhanced lipolysis ( J:171262 )