All Phenotypes Srsf2<tm1Xdfu> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Myl2^tm1(cre)Krc/Myl2⁺ Srsf2^tm1Xdfu/Srsf2^tm1Xdfu	involves: 129S4/SvJae	MGI:3037209
cn2	Srsf2^tm1Xdfu/Srsf2⁺ Tg(Lck-cre)I57Jxm/?	involves: 129S4/SvJae * 129X1/SvJ * ICR	MGI:3037204
cn3	Srsf2^tm1Xdfu/Srsf2^tm1Xdfu Tg(Lck-cre)I57Jxm/?	involves: 129S4/SvJae * 129X1/SvJ * ICR	MGI:3037198
cn4	Srsf2^tm1Xdfu/Srsf2^tm1Xdfu Tg(Mx1-cre)1Cgn/?	involves: 129S4/SvJae * C57BL/6J * CBA/J	MGI:5695464

Allelic Composition	Myl2^tm1(cre)Krc/Myl2⁺ Srsf2^tm1Xdfu/Srsf2^tm1Xdfu
Genetic Background	involves: 129S4/SvJae

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myl2^tm1(cre)Krc mutation (2 available); any Myl2 mutation (22 available)
Srsf2^tm1Xdfu mutation (1 available); any Srsf2 mutation (11 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

pregnancy-related premature death ( J:88432 )

• life span is normal except that pregnancy-induced mortality is seen

cardiovascular system

cardiovascular system phenotype ( J:88432 )

N	• deletion had little affect on cardiomyocyte proliferation or viability • mutants have a normal heart rate, interventricular septum thickness, and left ventricular posterior wall thickness

cardiac interstitial fibrosis ( J:88432 )

• extensive fibrosis and myofibril disarray are evident by trichome staining in older mice

dilated cardiomyopathy ( J:88432 )

• mutant hearts appear normal initially and become enlarged 5 weeks after birth

• increasing workload and stress results in manifestation of a latent defect in cardiac function

decreased ventricle muscle contractility ( J:88432 )

• mutants have a severe contraction defect

• both left ventricular end-diastolic dimensions and end-systolic dimensions are markedly increased 5 to 6 weeks after birth

• the percent fractional shortening and mean velocity of circumferential fiber shortening (indicators of systolic cardiac function) are severely reduced

• in isolated cardiomyocytes resting intracellular and diastolic Ca²⁺ is normal however, with increasing pacing frequencies a significant decrease in both Ca²⁺ transients and cell contraction are detected

• defects in Ca²⁺ relaxation kinetics and reduced Ca²⁺ transients would weaken the excitation-contraction coupling in response to increasing workload

muscle

dilated cardiomyopathy ( J:88432 )

• mutant hearts appear normal initially and become enlarged 5 weeks after birth

• increasing workload and stress results in manifestation of a latent defect in cardiac function

decreased ventricle muscle contractility ( J:88432 )

• mutants have a severe contraction defect

• both left ventricular end-diastolic dimensions and end-systolic dimensions are markedly increased 5 to 6 weeks after birth

• the percent fractional shortening and mean velocity of circumferential fiber shortening (indicators of systolic cardiac function) are severely reduced

• defects in Ca²⁺ relaxation kinetics and reduced Ca²⁺ transients would weaken the excitation-contraction coupling in response to increasing workload

cellular

cardiac interstitial fibrosis ( J:88432 )

• extensive fibrosis and myofibril disarray are evident by trichome staining in older mice

Allelic Composition	Srsf2^tm1Xdfu/Srsf2⁺ Tg(Lck-cre)I57Jxm/?
Genetic Background	involves: 129S4/SvJae * 129X1/SvJ * ICR

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Srsf2^tm1Xdfu mutation (1 available); any Srsf2 mutation (11 available)
Tg(Lck-cre)I57Jxm mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased thymus weight ( J:88668 )

• a modest reduction in thymic weight is seen

decreased thymocyte number ( J:88668 )

• a modest reduction in the total number of thymocytes is seen

immune system

decreased thymus weight ( J:88668 )

• a modest reduction in thymic weight is seen

decreased thymocyte number ( J:88668 )

• a modest reduction in the total number of thymocytes is seen

endocrine/exocrine glands

decreased thymus weight ( J:88668 )

• a modest reduction in thymic weight is seen

decreased thymocyte number ( J:88668 )

• a modest reduction in the total number of thymocytes is seen

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

absent thymus corticomedullary boundary ( J:88668 )

• in histological sections the cortical-medullary junction is absent

small thymus ( J:88668 )

• the size and weight of the thymus is about 10% to 30% that in wild-types

• other peripheral lymphoid organs appear normal

abnormal T cell differentiation ( J:88668 )

• in T cells sorted by stage cells with the gene deleted are progressively eliminated

• splicing of CD45 is abnormal with a significant percentage of single positive and double positive T cells expressing CD45 isoforms missing exon 5

increased double-negative T cell number ( J:88668 )

• the percentage of double-negative T cells could be elevated up to 40% of the total (compared to 6% in wild-type)

• T cell development is arrested at the CD44^-CD25⁺ stage

decreased T cell number ( J:88668 )

• the absolute number of single positive T cells is greatly reduced

decreased thymocyte number ( J:88668 )

• a dramatic reduction in total thymocytes is seen

decreased double-positive T cell number ( J:88668 )

• the percentage of double-positive T cells could be decreased down to 50% of the total (compared to 85% in wild-type)

abnormal spleen white pulp morphology ( J:88668 )

• the white pulp is paler than normal

• the absolute number of single positive T cells is significantly reduced

• none of the mature T cells found in the spleen have the gene deleted

• the spleen is otherwise unaffected

immune system

absent thymus corticomedullary boundary ( J:88668 )

• in histological sections the cortical-medullary junction is absent

small thymus ( J:88668 )

• the size and weight of the thymus is about 10% to 30% that in wild-types

• other peripheral lymphoid organs appear normal

abnormal T cell differentiation ( J:88668 )

• in T cells sorted by stage cells with the gene deleted are progressively eliminated

• splicing of CD45 is abnormal with a significant percentage of single positive and double positive T cells expressing CD45 isoforms missing exon 5

increased double-negative T cell number ( J:88668 )

• the percentage of double-negative T cells could be elevated up to 40% of the total (compared to 6% in wild-type)

• T cell development is arrested at the CD44^-CD25⁺ stage

decreased double-positive T cell number ( J:88668 )

• the percentage of double-positive T cells could be decreased down to 50% of the total (compared to 85% in wild-type)

decreased T cell number ( J:88668 )

• the absolute number of single positive T cells is greatly reduced

decreased thymocyte number ( J:88668 )

• a dramatic reduction in total thymocytes is seen

abnormal spleen white pulp morphology ( J:88668 )

• the white pulp is paler than normal

• the absolute number of single positive T cells is significantly reduced

• none of the mature T cells found in the spleen have the gene deleted

• the spleen is otherwise unaffected

endocrine/exocrine glands

absent thymus corticomedullary boundary ( J:88668 )

• in histological sections the cortical-medullary junction is absent

small thymus ( J:88668 )

• the size and weight of the thymus is about 10% to 30% that in wild-types

• other peripheral lymphoid organs appear normal

decreased thymocyte number ( J:88668 )

• a dramatic reduction in total thymocytes is seen

Allelic Composition	Srsf2^tm1Xdfu/Srsf2^tm1Xdfu Tg(Mx1-cre)1Cgn/?
Genetic Background	involves: 129S4/SvJae * C57BL/6J * CBA/J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Srsf2^tm1Xdfu mutation (1 available); any Srsf2 mutation (11 available)
Tg(Mx1-cre)1Cgn mutation (7 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

impaired hematopoiesis ( J:221404 )

abnormal hematopoietic system physiology ( J:221404 )

• leukopenia is observed in bone marrow of lethally irritated recipient mice 18 weeks post-transplantation (pIpC injection 4 weeks after transplantation)

• anemia is observed 18 weeks post-bone marrow transplantation (pIpC injection 4 weeks after transplantation)

• hemoglobin is decreased donor bone marrow post-transplantation

• impaired reconstitution of hematopoietic stem/progenitor cells in peripheral blood in competitive bone marrow transplantation assay 14 weeks post-pIpC injection

• decrease in total hematopoietic stem cell (LSK) cell number in bone marrow in competitive bone marrow transplantation assay 14 weeks post-pIpC injection

• decrease in myeloid progenitor total cell number in bone marrow in competitive bone marrow transplantation assay 14 weeks post-pIpC injection

bone marrow failure ( J:221404 )

• bone marrow aplasia is observed 14 weeks post pIpC injection

immune system

bone marrow failure ( J:221404 )

• bone marrow aplasia is observed 14 weeks post pIpC injection

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/16/2024 MGI 6.23