Phenotypes associated with this allele
Allelic
Composition |
Rac1tm2Tyb/Rac1tm2Tyb
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Genetic
Background |
involves: 129S4/SvJae * C3H * C57BL/6 * CD-1 |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rac1tm2Tyb mutation
(0 available);
any
Rac1 mutation
(21 available)
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mortality/aging
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• most arrest before E7.5 and all arrest by E7.5
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embryo
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• expression analysis indicates anterior visceral endoderm cells fail to migrate from the distal tip to the embryonic-extraembryonic boundary by E6.5
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• most arrest before E7.5 and all arrest by E7.5
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• primitive streak formation is ectopically initiated at all positions around the circumference of most embryos
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• constriction at the boundary between embryonic and extraembryonic regions in mice that survive to E7.5
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cellular
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• expression analysis indicates anterior visceral endoderm cells fail to migrate from the distal tip to the embryonic-extraembryonic boundary by E6.5
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rac1tm2Tyb mutation
(0 available);
any
Rac1 mutation
(21 available)
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mortality/aging
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• homozygous embryos disappear by E11.5
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Edil3Tg(Sox2-cre)1Amc mutation
(5 available);
any
Edil3 mutation
(42 available)
Rac1tm1Tyb mutation
(0 available);
any
Rac1 mutation
(21 available)
Rac1tm2Tyb mutation
(0 available);
any
Rac1 mutation
(21 available)
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mortality/aging
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• survive longer than germ line null mice
• survive until E8.5
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embryo
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• in the mesoderm and basal regions of the epiblast at E7.5
• at E6.5 and E7.5, cell death is increased in the anterior half of the embryo compared to the posterior half
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• expression analysis indicates organization of midline structures is abnormal
• the midline is broad at E8.5
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• mesoderm cells are specified but migration away from the primitive streak is impaired
• mesodermal cells are round, with just a few short protrusions
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• the embryonic region is smaller compared to control littermates from E7.5 onwards
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• expression analysis indicates the notochordal plate is wider and shorter at E7.75
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• the neural plate is present but fails to initiate neural tube closure
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• during epithelial to mesenchymal transition in the primitive streak breakdown of the basement membrane appears somewhat disrupted with large laminin aggregates seen on nascent mesodermal cells
• accumulation of T expressing cells in the primitive streak indicating an impairment in migration of cells away from the streak
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• node cells are specified but fail to coalesce to form a normal node
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• properly organized somites do not form
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cellular
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• cultured cells from E7.5 embryos fail to adhere to either fibronectin or Matrigel matrices
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• in the mesoderm and basal regions of the epiblast at E7.5
• at E6.5 and E7.5, cell death is increased in the anterior half of the embryo compared to the posterior half
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• decrease in the mitotic index in E7.5 embryos
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cardiovascular system
growth/size/body
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• the embryonic region is smaller compared to control littermates from E7.5 onwards
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nervous system
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• the neural plate is present but fails to initiate neural tube closure
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rac1tm1Tyb mutation
(0 available);
any
Rac1 mutation
(21 available)
Rac1tm2Tyb mutation
(0 available);
any
Rac1 mutation
(21 available)
Tg(Ttr-cre)1Hadj mutation
(0 available)
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mortality/aging
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• survive longer than germ line null mice, up to E8.25
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embryo
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• migration of anterior visceral endoderm cells is disrupted
• defect in migration is not as severe as in germ line null mice probably due to the timing of cre expression
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• expression analysis indicates a defect in the initial specification of the AP axis in most embryos
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• constriction at the boundary between embryonic and extraembryonic regions
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• visceral endoderm cells fail to form a border around the midline structures
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craniofacial
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• abnormal headfolds at E7.5
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cellular
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• migration of anterior visceral endoderm cells is disrupted
• defect in migration is not as severe as in germ line null mice probably due to the timing of cre expression
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Edil3Tg(Sox2-cre)1Amc mutation
(5 available);
any
Edil3 mutation
(42 available)
Ptentm1Ppp mutation
(0 available);
any
Pten mutation
(81 available)
Rac1tm1Tyb mutation
(0 available);
any
Rac1 mutation
(21 available)
Rac1tm2Tyb mutation
(0 available);
any
Rac1 mutation
(21 available)
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embryo
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• embryo morphology is stated to be indistinguishable from that of mutant mice wild-type for Pten
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cellular
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• cell death is reduced compared to mutant mice wild-type for Pten
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rac1tm2Tyb mutation
(0 available);
any
Rac1 mutation
(21 available)
Tg(Hhex-EGFP)#Rbe mutation
(0 available)
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embryo
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• anterior visceral endoderm cells fail to migrate from the distal tip to the embryonic-extraembryonic boundary by E6.5
• between E5.5 and E5.75 some cells lose contact with the basement membrane
• at E6.5 cells that fail to migrate form a multilayered epithelium
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cellular
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• anterior visceral endoderm cells fail to migrate from the distal tip to the embryonic-extraembryonic boundary by E6.5
• between E5.5 and E5.75 some cells lose contact with the basement membrane
• at E6.5 cells that fail to migrate form a multilayered epithelium
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Analysis Tools