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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Grhl3tm1Jane
targeted mutation 1, Stephen M Jane
MGI:2684328
Summary 15 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Grhl3tm1Jane/Grhl3tm1Jane involves: 129S1/Sv MGI:4836390
hm2
 		Grhl3tm1Jane/Grhl3tm1Jane involves: 129S1/Sv * C57BL/6 MGI:2684377
ht3
 		Grhl3tm1Jane/Grhl3+ involves: 129S1/Sv MGI:5306662
ht4
 		Grhl3tm1Jane/Grhl3tm2.1Jane involves: 129S1/Sv * BALB/cJ MGI:4836393
ht5
 		Grhl3ct/Grhl3tm1Jane involves: 129S1/Sv * C57BL/6 * STOCK ct/J MGI:2684380
cn6
 		Grhl1tm1Jane/Grhl1tm1Jane
Grhl3tm1Jane/Grhl3tm3.1Jane
Tg(KRT14-cre/ERT)20Efu/0 		involves: 129S1/Sv * C57BL/6 * CD-1 MGI:5896360
cn7
 		Grhl3tm1Jane/Grhl3tm3.1Jane
Tg(MMTV-cre)4Mam/0 		involves: 129S1/Sv * C57BL/6 * FVB MGI:5306660
cn8
 		Grhl1tm1Jane/Grhl1tm1Jane
Grhl3tm1Jane/Grhl3tm3.1Jane
Tg(KRT14-cre)8Brn/0 		involves: 129S1/Sv * C57BL/6 * FVB/N MGI:5896359
cn9
 		Grhl3tm1Jane/Grhl3tm3.1Jane
Tg(KRT14-cre)8Brn/0 		involves: 129S1/Sv * C57BL/6 * FVB/N MGI:5306659
cn10
 		Grhl3tm1Jane/Grhl3tm3.1Jane
Tgm5tm2a(KOMP)Wtsi/Tgm5tm2a(KOMP)Wtsi
Tg(KRT14-cre)8Brn/0 		involves: 129S1/Sv * C57BL/6N * FVB/N MGI:5896358
cx11
 		Grhl3tm1Jane/Grhl3+
Ptentm1Mak/Pten+ 		involves: 129P2/OlaHsd * 129S1/Sv MGI:5306661
cx12
 		Grhl2tm1.1Jane/Grhl2tm1.1Jane
Grhl3tm1Jane/Grhl3+ 		involves: 129S1/Sv * BALB/cJ * C57BL/6 MGI:4836389
cx13
 		Grhl2tm1.1Jane/Grhl2+
Grhl3tm1Jane/Grhl3+ 		involves: 129S1/Sv * BALB/cJ * C57BL/6 MGI:4836386
cx14
 		Grhl2tm1.1Jane/Grhl2tm1.1Jane
Grhl3tm1Jane/Grhl3tm1Jane 		involves: 129S1/Sv * BALB/cJ * C57BL/6 MGI:4836385
cx15
 		Grhl2tm1.1Jane/Grhl2+
Grhl3tm1Jane/Grhl3tm1Jane 		involves: 129S1/Sv * BALB/cJ * C57BL/6 MGI:4836387


Genotype
MGI:4836390
hm1
Allelic
Composition		 Grhl3tm1Jane/Grhl3tm1Jane
Genetic
Background		 involves: 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
spina bifida ( J:164805 )
• fully penetrant thoraco-lumbo-sacral spina bifida

skeleton
abnormal vertebral pedicle morphology ( J:164805 )
• splayed vertebral pedicles are seen at the level of T7

embryo
spina bifida ( J:164805 )
• fully penetrant thoraco-lumbo-sacral spina bifida

cellular
increased keratinocyte proliferation ( J:178952 )
• in vivo and cultured keratinocyte from E18.5 mice with increased colony numbers in soft agar

integument
increased keratinocyte proliferation ( J:178952 )
• in vivo and cultured keratinocyte from E18.5 mice with increased colony numbers in soft agar
thick epidermis ( J:178952 )
• at E18.5




Genotype
MGI:2684377
hm2
Allelic
Composition		 Grhl3tm1Jane/Grhl3tm1Jane
Genetic
Background		 involves: 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
preweaning lethality, complete penetrance ( J:86772 )
• mutant embryos were represented in Mendelian ratios up to E18.5; no mice survived to weaning

integument

embryo
decreased embryo size ( J:86772 )
• embryos show growth retardation
spina bifida ( J:86772 )
• all embryos had thoracolumbosacral spina bifida and curled tails
• the neural plate appeared to furrow normally, but neural foled elevation did not occur
• the incidence of spina bifida could not be reduced with folate treatment

growth/size/body
decreased embryo size ( J:86772 )
• embryos show growth retardation

limbs/digits/tail

skeleton
abnormal vertebrae morphology ( J:86772 )
• full body skeletal preparations showed abnormalities in the vertebral column
absent vertebral arch ( J:86772 )
• lack of vertebral arch formation

nervous system
spina bifida ( J:86772 )
• all embryos had thoracolumbosacral spina bifida and curled tails
• the neural plate appeared to furrow normally, but neural foled elevation did not occur
• the incidence of spina bifida could not be reduced with folate treatment
exencephaly ( J:86772 )
• 2% of mutants had coincident exencephaly




Genotype
MGI:5306662
ht3
Allelic
Composition		 Grhl3tm1Jane/Grhl3+
Genetic
Background		 involves: 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
neoplasm
neoplasm ( J:178952 )
N
• mice are almost completely safe-guarded against skin squamous cell carcinoma formation




Genotype
MGI:4836393
ht4
Allelic
Composition		 Grhl3tm1Jane/Grhl3tm2.1Jane
Genetic
Background		 involves: 129S1/Sv * BALB/cJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
Grhl3tm2.1Jane mutation (1 available); any Grhl3 mutation (53 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging

nervous system
spina bifida ( J:164805 )
• in the lumbo-sacral region in almost all embryos

embryo
spina bifida ( J:164805 )
• in the lumbo-sacral region in almost all embryos




Genotype
MGI:2684380
ht5
Allelic
Composition		 Grhl3ct/Grhl3tm1Jane
Genetic
Background		 involves: 129S1/Sv * C57BL/6 * STOCK ct/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl3ct mutation (2 available); any Grhl3 mutation (53 available)
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
spina bifida ( J:86772 )
• the incidence of spina bifida in these embryos was higher than reported for ct homozygotes (31% vs 12%, respectively)
• the extent of the spina bifida more closely resembled ct (lumbosacral spina bifida) than the null allele (thorocolumbosacral)
• 14% of the mice had tail flexion defects only
• 5% of mice with this genotype were normal

embryo
spina bifida ( J:86772 )
• the incidence of spina bifida in these embryos was higher than reported for ct homozygotes (31% vs 12%, respectively)
• the extent of the spina bifida more closely resembled ct (lumbosacral spina bifida) than the null allele (thorocolumbosacral)
• 14% of the mice had tail flexion defects only
• 5% of mice with this genotype were normal




Genotype
MGI:5896360
cn6
Allelic
Composition		 Grhl1tm1Jane/Grhl1tm1Jane
Grhl3tm1Jane/Grhl3tm3.1Jane
Tg(KRT14-cre/ERT)20Efu/0
Genetic
Background		 involves: 129S1/Sv * C57BL/6 * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl1tm1Jane mutation (1 available); any Grhl1 mutation (33 available)
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
Grhl3tm3.1Jane mutation (1 available); any Grhl3 mutation (53 available)
Tg(KRT14-cre/ERT)20Efu mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
postnatal lethality, complete penetrance ( J:233531 )
• all die within 7 weeks of tamoxifen treatment

integument
impaired skin barrier function ( J:233531 )
• regression of skin barrier function after tamoxifen treatment
• dye penetration is variegated matching the pattern of cre mediated gene deletion
abnormal corneocyte envelope morphology ( J:233531 )
• after tamoxifen treatment gross abnormalities and increased fragility are seen
thick epidermis ( J:233531 )
• after tamoxifen treatment

homeostasis/metabolism
impaired skin barrier function ( J:233531 )
• regression of skin barrier function after tamoxifen treatment
• dye penetration is variegated matching the pattern of cre mediated gene deletion




Genotype
MGI:5306660
cn7
Allelic
Composition		 Grhl3tm1Jane/Grhl3tm3.1Jane
Tg(MMTV-cre)4Mam/0
Genetic
Background		 involves: 129S1/Sv * C57BL/6 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
Grhl3tm3.1Jane mutation (1 available); any Grhl3 mutation (53 available)
Tg(MMTV-cre)4Mam mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
integument
abnormal skin morphology ( J:178952 )
• authors state that mice exhibit a phenotype identical to Grhl3tm1Jane/Grhl3tm3.1Jane Tg(KRT14-cre)8Brn mice
abnormal skin physiology ( J:178952 )
• authors state that mice exhibit a phenotype identical to Grhl3tm1Jane/Grhl3tm3.1Jane Tg(KRT14-cre)8Brn mice

neoplasm
increased tumor incidence ( J:178952 )
• authors state that mice exhibit a phenotype identical to Grhl3tm1Jane/Grhl3tm3.1Jane Tg(KRT14-cre)8Brn mice




Genotype
MGI:5896359
cn8
Allelic
Composition		 Grhl1tm1Jane/Grhl1tm1Jane
Grhl3tm1Jane/Grhl3tm3.1Jane
Tg(KRT14-cre)8Brn/0
Genetic
Background		 involves: 129S1/Sv * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl1tm1Jane mutation (1 available); any Grhl1 mutation (33 available)
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
Grhl3tm3.1Jane mutation (1 available); any Grhl3 mutation (53 available)
Tg(KRT14-cre)8Brn mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
postnatal lethality, complete penetrance ( J:233531 )
• present in expected numbers at E18.5 but all died by P3 with most lost during the first 48 hours after birth

integument
impaired skin barrier function ( J:233531 )
• at P2 excessive fluid loss and regression of skin barrier function leads to dehydration and death
abnormal corneocyte envelope morphology ( J:233531 )
• loss of cornified envelope integrity by P2

homeostasis/metabolism
impaired skin barrier function ( J:233531 )
• at P2 excessive fluid loss and regression of skin barrier function leads to dehydration and death




Genotype
MGI:5306659
cn9
Allelic
Composition		 Grhl3tm1Jane/Grhl3tm3.1Jane
Tg(KRT14-cre)8Brn/0
Genetic
Background		 involves: 129S1/Sv * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
Grhl3tm3.1Jane mutation (1 available); any Grhl3 mutation (53 available)
Tg(KRT14-cre)8Brn mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
integument
integument phenotype ( J:178952 , J:233531 )
N
• from E18.5 through 8 weeks, mice exhibit normal skin barrier formation (J:178952)
• no overt skin barrier defect or abnormality of the cornified envelope of the stratum corneum from birth to 6 weeks of age (J:233531)
epidermal hyperplasia ( J:178952 )
• in aged mice
increased skin papilloma incidence ( J:178952 )
• spontaneously in aged mice affecting the snout and neck
• in 4 weeks after DMBA/TPA treatment
increased skin squamous cell carcinoma incidence ( J:178952 )
• spontaneously in aged mice affecting the snout and neck
• in 4 weeks after DMBA/TPA treatment

neoplasm
increased incidence of tumors by chemical induction ( J:178952 )
• 4 weeks after DMBA/TPA treatment, mice develop skin papillomas many which progress to squamous cell carcinomas unlike control mice
increased skin papilloma incidence ( J:178952 )
• spontaneously in aged mice affecting the snout and neck
• in 4 weeks after DMBA/TPA treatment
increased skin squamous cell carcinoma incidence ( J:178952 )
• spontaneously in aged mice affecting the snout and neck
• in 4 weeks after DMBA/TPA treatment

homeostasis/metabolism
increased incidence of tumors by chemical induction ( J:178952 )
• 4 weeks after DMBA/TPA treatment, mice develop skin papillomas many which progress to squamous cell carcinomas unlike control mice




Genotype
MGI:5896358
cn10
Allelic
Composition		 Grhl3tm1Jane/Grhl3tm3.1Jane
Tgm5tm2a(KOMP)Wtsi/Tgm5tm2a(KOMP)Wtsi
Tg(KRT14-cre)8Brn/0
Genetic
Background		 involves: 129S1/Sv * C57BL/6N * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
Grhl3tm3.1Jane mutation (1 available); any Grhl3 mutation (53 available)
Tg(KRT14-cre)8Brn mutation (3 available)
Tgm5tm2a(KOMP)Wtsi mutation (1 available); any Tgm5 mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
mortality/aging ( J:233531 )
N
• present in expected numbers at E18.5 and 6 weeks of age

integument
integument phenotype ( J:233531 )
N
• no evidence of skin barrier dysfunction was seen




Genotype
MGI:5306661
cx11
Allelic
Composition		 Grhl3tm1Jane/Grhl3+
Ptentm1Mak/Pten+
Genetic
Background		 involves: 129P2/OlaHsd * 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
Ptentm1Mak mutation (1 available); any Pten mutation (86 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
integument
increased skin papilloma incidence ( J:178952 )
• in DMBA/TPA treated mice
increased skin squamous cell carcinoma incidence ( J:178952 )
• in DMBA/TPA treated mice to a greater extent than in either single heterozygote

neoplasm
increased skin papilloma incidence ( J:178952 )
• in DMBA/TPA treated mice
increased skin squamous cell carcinoma incidence ( J:178952 )
• in DMBA/TPA treated mice to a greater extent than in either single heterozygote




Genotype
MGI:4836389
cx12
Allelic
Composition		 Grhl2tm1.1Jane/Grhl2tm1.1Jane
Grhl3tm1Jane/Grhl3+
Genetic
Background		 involves: 129S1/Sv * BALB/cJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl2tm1.1Jane mutation (2 available); any Grhl2 mutation (42 available)
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
spina bifida ( J:164805 )
• fully penetrant thoraco-lumbo-sacral spina bifida
• a small region of the neural tube between the midthoracic region and the hindbrain/cervical cord boundary is closed

skeleton
abnormal vertebral pedicle morphology ( J:164805 )
• splayed vertebral pedicles are seen at the level of T11 in the most severely affected embryos

craniofacial
facial cleft ( J:164805 )
• failure of cranio-facial fusion at E10.5

embryo
spina bifida ( J:164805 )
• fully penetrant thoraco-lumbo-sacral spina bifida
• a small region of the neural tube between the midthoracic region and the hindbrain/cervical cord boundary is closed

growth/size/body
facial cleft ( J:164805 )
• failure of cranio-facial fusion at E10.5




Genotype
MGI:4836386
cx13
Allelic
Composition		 Grhl2tm1.1Jane/Grhl2+
Grhl3tm1Jane/Grhl3+
Genetic
Background		 involves: 129S1/Sv * BALB/cJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl2tm1.1Jane mutation (2 available); any Grhl2 mutation (42 available)
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal neural tube morphology ( J:164805 )
• neural tube defects of varying severity are seen in 13 percent of embryos
exencephaly ( J:164805 )
• exencephaly involving only the mid- and hindbrain is seen in 5 percent of embryos

skeleton
abnormal vertebral pedicle morphology ( J:164805 )
• splayed vertebral pedicles are seen at the level of T11 in the most severely affected embryos

limbs/digits/tail
curly tail ( J:164805 )
• seen in a few embryos, either alone or in combination with spina bifida or exencephaly

embryo
abnormal neural tube morphology ( J:164805 )
• neural tube defects of varying severity are seen in 13 percent of embryos




Genotype
MGI:4836385
cx14
Allelic
Composition		 Grhl2tm1.1Jane/Grhl2tm1.1Jane
Grhl3tm1Jane/Grhl3tm1Jane
Genetic
Background		 involves: 129S1/Sv * BALB/cJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl2tm1.1Jane mutation (2 available); any Grhl2 mutation (42 available)
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
craniofacial
facial cleft ( J:164805 )
• failure of cranio-facial fusion at E10.5

embryo
spina bifida ( J:164805 )
• fully penetrant thoraco-lumbo-sacral spina bifida
• a small region of the neural tube between the midthoracic region and the hindbrain/cervical cord boundary is closed

nervous system
spina bifida ( J:164805 )
• fully penetrant thoraco-lumbo-sacral spina bifida
• a small region of the neural tube between the midthoracic region and the hindbrain/cervical cord boundary is closed

skeleton

growth/size/body
facial cleft ( J:164805 )
• failure of cranio-facial fusion at E10.5




Genotype
MGI:4836387
cx15
Allelic
Composition		 Grhl2tm1.1Jane/Grhl2+
Grhl3tm1Jane/Grhl3tm1Jane
Genetic
Background		 involves: 129S1/Sv * BALB/cJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grhl2tm1.1Jane mutation (2 available); any Grhl2 mutation (42 available)
Grhl3tm1Jane mutation (1 available); any Grhl3 mutation (53 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
spina bifida ( J:164805 )
• fully penetrant thoraco-lumbo-sacral spina bifida
exencephaly ( J:164805 )
• fully penetrant mid and hindbrain exencephaly
• however, forebrain closure is normal

skeleton
abnormal vertebral pedicle morphology ( J:164805 )
• splayed vertebral pedicles are seen at the level of T11 in the most severely affected embryos

craniofacial
craniofacial phenotype ( J:164805 )
N
• unlike in mice homozygous for Grhl2tm1.1Jane facial fusion is normal

embryo
spina bifida ( J:164805 )
• fully penetrant thoraco-lumbo-sacral spina bifida




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09/30/2025
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