Phenotypes associated with this allele

• Allele Symbol: Becn1^tm1Blev
• Allele Name: targeted mutation 1, Beth Levine
• Allele ID: MGI:2683696
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Becn1^tm1Blev/Becn1^tm1Blev	involves: 129X1/SvJ	MGI:5471806
hm2	Becn1^tm1Blev/Becn1^tm1Blev	involves: 129X1/SvJ * C57BL/6J	MGI:3837447
ht3	Becn1^tm1Blev/Becn1^+	involves: 129X1/SvJ * C57BL/6J	MGI:2683698
cx4	Becn1^tm1Blev/Becn1^+ Tg(Alb1HBV)44Bri/0	involves: 129X1/SvJ * C57BL/6J	MGI:2683697
cx5	Becn1^tm1Blev/Becn1^+ Tg(CAG-EGFP/Map1lc3b)53Nmz/0	involves: 129X1/SvJ * C57BL/6J * C57BL/6NCrj	MGI:3837449
cx6	Becn1^tm1Blev/Becn1^+ Tg(CAG-EGFP/Map1lc3b)53Nmz/0	involves: 129X1/SvJ * C57BL/6NCrj	MGI:5313436

Genotype
MGI:5471806

hm1

• Allelic Composition: Becn1^tm1Blev/Becn1^tm1Blev
• Genetic Background: involves: 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

decreased myocardial infarct size ( J:186597 )

• following ischemia and reperfusion with SB216763 (a GSK3b inhibitor) and rapamycin treatment

increased myocardial infarct size ( J:186597 )

• following 2 hours of ischemia without reperfusion

• not affected by treatment with SB216763 (a GSK3b inhibitor) and rapamycin

homeostasis/metabolism

decreased myocardial infarct size ( J:186597 )

• following ischemia and reperfusion with SB216763 (a GSK3b inhibitor) and rapamycin treatment

increased myocardial infarct size ( J:186597 )

• following 2 hours of ischemia without reperfusion

• not affected by treatment with SB216763 (a GSK3b inhibitor) and rapamycin

Genotype
MGI:3837447

hm2

• Allelic Composition: Becn1^tm1Blev/Becn1^tm1Blev
• Genetic Background: involves: 129X1/SvJ * C57BL/6J

mortality/aging

prenatal lethality, complete penetrance ( J:86953 )

• embryonic lethality, time not specified

Genotype
MGI:2683698

ht3

• Allelic Composition: Becn1^tm1Blev/Becn1⁺
• Genetic Background: involves: 129X1/SvJ * C57BL/6J

mortality/aging

decreased tumor-free survival time ( J:86953 )

• mutants show some tumor-associated mortality at 6 to 8 months of age

homeostasis/metabolism

impaired autophagy ( J:86953 )

• germinal center B lymphocytes have fewer autophagic vacuoles than wild-type lymphocytes 10 days after immunization with sheep red blood cells, indicating reduced autophagic activity

neoplasm

increased tumor incidence ( J:86953 )

• 15% of mutants at 13-18 months of age exhibit palpable tumors compared to 1% of wild-type mice

increased mammary gland tumor incidence ( J:86953 )

• proliferative lesions (mammary intraepithelial neoplasia, adenomyoepithelioma, and acinar neoplasia) are present in mammary gland of 13 to 18 month old mutants

increased lung adenoma incidence ( J:86953 )

• mutants have an increased frequency of lung lesions in earlier stages of neoplasia, including hyperplastic alveolar epithelium resembling human atypical adenomatous hyperplasia and bronchioalveolar adenomas composed of monomorphous cells with minimal cytologic atypia

increased lymphoma incidence ( J:86953 )

• develop lymphomas that occur more frequently, at an earlier age, are more likely to present as palpable masses than in wild-type, including diffuse large-cell lymphoma, follicular lymphoma, immunoblastic variant of diffuse large-cell lymphoma, anaplastic plasmacytoma, and histiocytic sarcoma

increased follicular lymphoma incidence ( J:86953 )

increased histiocytic sarcoma incidence ( J:86953 )

increased malignant tumor incidence ( J:86953 )

• mutants have a higher probability of having a malignancy (30%) than wild-type (14%); malignancies do not contain mutations in the wild-type Becn1 allele

increased hepatocellular carcinoma incidence ( J:86953 )

• well-differentiated hepatocellular carcinomas

increased lung carcinoma incidence ( J:86953 )

• greater number of and larger lung carcinomas than in wild-type mice

increased lung adenocarcinoma incidence ( J:86953 )

• lung cancers show features of well-differentiated human papillary adenocarcinomas

increased plasmacytoma incidence ( J:86953 )

• anaplastic plasmacytoma

liver/biliary system

increased hepatocellular carcinoma incidence ( J:86953 )

• well-differentiated hepatocellular carcinomas

respiratory system

increased lung adenoma incidence ( J:86953 )

• mutants have an increased frequency of lung lesions in earlier stages of neoplasia, including hyperplastic alveolar epithelium resembling human atypical adenomatous hyperplasia and bronchioalveolar adenomas composed of monomorphous cells with minimal cytologic atypia

increased lung carcinoma incidence ( J:86953 )

• greater number of and larger lung carcinomas than in wild-type mice

increased lung adenocarcinoma incidence ( J:86953 )

• lung cancers show features of well-differentiated human papillary adenocarcinomas

cellular

impaired autophagy ( J:86953 )

• germinal center B lymphocytes have fewer autophagic vacuoles than wild-type lymphocytes 10 days after immunization with sheep red blood cells, indicating reduced autophagic activity

endocrine/exocrine glands

abnormal mammary gland duct morphology ( J:86953 )

♀ • mammary ducts are frequently abnormal, with multiple layers of epithelial cells and intraluminal collections of epithelial cells

♀ • hyperproliferation of mammary ductal epithelial cells in the terminal end buds

♀ • mammary ducts show increase in DNA synthesis

increased mammary gland tumor incidence ( J:86953 )

• proliferative lesions (mammary intraepithelial neoplasia, adenomyoepithelioma, and acinar neoplasia) are present in mammary gland of 13 to 18 month old mutants

hematopoietic system

increased spleen germinal center number ( J:86953 )

• mutants show an increase in the number of germinal centeres in the spleens following immunization with sheep red blood cells at 2 months of age

increased spleen germinal center size ( J:86953 )

• mutants show an increase in the size of germinal centers in the spleens following immunization with sheep red blood cells at 2 months of age

immune system

abnormal immune system morphology ( J:86953 )

• increase in frequency of extranodal, splenic, and nodal lymphoproliferative disease

increased spleen germinal center number ( J:86953 )

• mutants show an increase in the number of germinal centeres in the spleens following immunization with sheep red blood cells at 2 months of age

increased spleen germinal center size ( J:86953 )

• mutants show an increase in the size of germinal centers in the spleens following immunization with sheep red blood cells at 2 months of age

integument

abnormal mammary gland duct morphology ( J:86953 )

♀ • mammary ducts are frequently abnormal, with multiple layers of epithelial cells and intraluminal collections of epithelial cells

♀ • hyperproliferation of mammary ductal epithelial cells in the terminal end buds

♀ • mammary ducts show increase in DNA synthesis

increased mammary gland tumor incidence ( J:86953 )

• proliferative lesions (mammary intraepithelial neoplasia, adenomyoepithelioma, and acinar neoplasia) are present in mammary gland of 13 to 18 month old mutants

Genotype
MGI:2683697

cx4

• Allelic Composition: Becn1^tm1Blev/Becn1⁺; Tg(Alb1HBV)44Bri/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6J

neoplasm

increased tumor incidence ( J:86953 )

• mutants exhibit accelerated development of HBV-induced premalignant lesions compared to hemizygous Tg(Alb1HBV)44Bri mice

Genotype
MGI:3837449

cx5

• Allelic Composition: Becn1^tm1Blev/Becn1⁺; Tg(CAG-EGFP/Map1lc3b)53Nmz/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6J * C57BL/6NCrj

cellular

impaired autophagy ( J:86953 )

• mutants show decreased autophagy during starvation conditions in muscle and bronchial epithelial cells

homeostasis/metabolism

impaired autophagy ( J:86953 )

• mutants show decreased autophagy during starvation conditions in muscle and bronchial epithelial cells

Genotype
MGI:5313436

cx6

• Allelic Composition: Becn1^tm1Blev/Becn1⁺; Tg(CAG-EGFP/Map1lc3b)53Nmz/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6NCrj

cellular

impaired autophagy ( J:181308 )

• reduced exercised-induced autophagy in skeletal muscles compared to in wild-type muscles

homeostasis/metabolism

impaired exercise endurance ( J:181308 )

• mice exhibit decreased maximal treadmill running distance compared with wild-type mice

impaired autophagy ( J:181308 )

• reduced exercised-induced autophagy in skeletal muscles compared to in wild-type muscles

behavior/neurological

impaired exercise endurance ( J:181308 )

• mice exhibit decreased maximal treadmill running distance compared with wild-type mice