Phenotypes associated with this allele

• Allele Symbol: Pparg^tm1.1Auw
• Allele Name: targeted mutation 1.1, Johan Auwerx
• Allele ID: MGI:2683413
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pparg^tm1.1Auw/Pparg^tm1.1Auw	B6.Cg-Pparg^tm1.1Auw	MGI:3831148
hm2	Pparg^tm1.1Auw/Pparg^tm1.1Auw	either: 129 or C57BL/6J or (involves: 129 * C57BL/6J)	MGI:3719401

Genotype
MGI:3831148

hm1

• Allelic Composition: Pparg^tm1.1Auw/Pparg^tm1.1Auw
• Genetic Background: B6.Cg-Pparg^tm1.1Auw

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

extended life span ( J:144344 )

• mice live 13% longer than wild-type mice when fed standard chow

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:144344 )

N • despite reductions in circulating cholesterol and triglyceride levels, mice exhibit normal levels of free fatty acids, glucose, insulin, leptin, and adiponectin

N • despite altered metabolism, the respiratory exchange ratio is normal

decreased circulating cholesterol level ( J:144344 )

• when fed standard chow but not when fed a high fat diet

decreased circulating triglyceride level ( J:144344 )

• when fed standard chow but not when fed a high fat diet

abnormal circadian temperature homeostasis ( J:144344 )

• when fed standard chow, mice exhibit an increase in temperature at the beginning of the dark phase compared to wild-type mice

• however, when fed a high fat diet mice exhibit normal body temperature

improved glucose tolerance ( J:144344 )

• when fed standard chow but not when fed a high fat diet

increased insulin sensitivity ( J:144344 )

• when fed standard chow but not when fed a high fat diet

growth/size/body

decreased body mass index ( J:144344 )

• when fed standard chow but not when fed a high fat diet

decreased body weight ( J:144344 )

• when fed standard chow, mice exhibit reduced weight gain compared to wild-type mice

• when fed standard chow, mice exhibit reduced fat and lean mass compared to wild-type mice

• however, when fed a high fat diet mice do not exhibit reduced weight

behavior/neurological

increased fluid intake ( J:144344 )

• when fed a standard chow, mice exhibit periods of increased water consumption compared to wild-type mice

• however, mice fed a high fat diet exhibit normal drinking behaviors

hyperactivity ( J:144344 )

• when fed a standard chow, mice exhibit increased activity at the onset of the dark period compared to wild-type mice

• however, mice fed a high fat diet exhibit normal activity levels

adipose tissue

decreased total fat pad weight ( J:144344 )

• at 12 months, mice fed standard chow exhibit reduced fat pads compared to wild-type mice

• however, mice fed a high fat diet do not exhibit decreased adipocity compared to wild-type mice

increased adipocyte glucose uptake ( J:144344 )

• increased 2-fold compared to in wild-type mice

skeleton

abnormal bone structure ( J:144344 )

• total bone area and bone mineral content are slightly increased compared to in wild-type mice

• however, bone mineral density is normal

cellular

increased adipocyte glucose uptake ( J:144344 )

• increased 2-fold compared to in wild-type mice

abnormal cell differentiation ( J:144344 )

• mouse embryonic fibroblasts treated with an adipogenic cocktail exhibit reduced differentiation into adipose cells with reduced triglyceride content compared to similarly treated wild-type cells

Genotype
MGI:3719401

hm2

• Allelic Composition: Pparg^tm1.1Auw/Pparg^tm1.1Auw
• Genetic Background: either: 129 or C57BL/6J or (involves: 129 * C57BL/6J)

adipose tissue

adipose tissue phenotype ( J:86705 )

N • mice express normal levels of Pparg in their white adipose tissue