About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(Ins2-CD80)3B7Flv
transgene insertion 3B7, Richard Flavell
MGI:2682826
Summary 9 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
 		H2-Ab1b-tm1Gru/H2-Ab1b-tm1Gru
Tg(HLA-DQA1,HLA-DQB1)1Dv/0
Tg(Ins2-CD80)3B7Flv/0 		B6.Cg-H2-Ab1b-tm1Gru Tg(HLA-DQA1,HLA-DQB1)1Dv Tg(Ins2-CD80)3B7Flv MGI:3623405
cx2
 		Tg(HLA-DQA1,HLA-DQB1)1Dv/0
Tg(Ins2-CD80)3B7Flv/0 		B6.Cg-Tg(HLA-DQA1,HLA-DQB1)1Dv Tg(Ins2-CD80)3B7Flv MGI:3623410
cx3
 		H2-Ab1b-tm1Gru/H2-Ab1b-tm1Gru
Tg(HLA-DQA1,HLA-DQB1)1Dv/0
Tg(Ins2-CD80)3B7Flv/0 		involves: 129S2/SvPas * C57BL/6 * C57BL/10SnJ * CBA MGI:3639135
cx4
 		H2-Ab1b-tm1Gru/H2-Ab1b-tm1Gru
Tg(HLA-DQA1,HLA-DQB1)1Dv/0
Tg(HLA-DRB1)31Dmz/0
Tg(Ins2-CD80)3B7Flv/0 		involves: 129S2/SvPas * C57BL/6 * C57BL/10SnJ * CBA * SJL MGI:3639133
cx5
 		H2-Ab1b-tm1Gru/H2-Ab1b-tm1Gru
Tg(Ins2-CD80)3B7Flv/0 		involves: 129S2/SvPas * C57BL/6 * CBA MGI:3639140
cx6
 		H2-Ab1b-tm1Gru/H2-Ab1b-tm1Gru
Tg(HLA-DRB1)31Dmz/0
Tg(Ins2-CD80)3B7Flv/0 		involves: 129S2/SvPas * C57BL/6 * CBA * SJL MGI:3639130
cx7
 		Tg(Ins2-CD80)3B7Flv/0
Tg(Ins2-Tnf)17Flv/0 		involves: C57BL/6 * CBA/Ca MGI:3766038
cx8
 		Tg(Ins2-CD80)3B7Flv/0
Tg(Ins2-H2-Ead,Ins2-H2-Eb1b)187-7Bri/0 		involves: C57BL/6 * CBA/Ca * SJL MGI:3766042
tg9
 		Tg(Ins2-CD80)3B7Flv/? involves: C57BL/6 * CBA/Ca * NOD/Caj MGI:3757580


Genotype
MGI:3623405
cx1
Allelic
Composition		 H2-Ab1b-tm1Gru/H2-Ab1b-tm1Gru
Tg(HLA-DQA1,HLA-DQB1)1Dv/0
Tg(Ins2-CD80)3B7Flv/0
Genetic
Background		 B6.Cg-H2-Ab1b-tm1Gru Tg(HLA-DQA1,HLA-DQB1)1Dv Tg(Ins2-CD80)3B7Flv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2-Ab1b-tm1Gru mutation (11 available); any H2-Ab1 mutation (81 available)
Tg(HLA-DQA1,HLA-DQB1)1Dv mutation (3 available)
Tg(Ins2-CD80)3B7Flv mutation (5 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
salivary gland inflammation ( J:59245 )
• diabetic transgenic mice display sialadenitis
increased susceptibility to autoimmune diabetes ( J:59245 )
• over 80% of transgenic mice develop diabetes (positive urine glucose test and >250 mg/dl blood glucose) beginning at 4 months of age

homeostasis/metabolism
increased circulating glucose level ( J:59245 )
• over 80% have blood glucose levels above 250 mg/dl
increased urine glucose level ( J:59245 )
• seen in over 80% of mice

digestive/alimentary system
salivary gland inflammation ( J:59245 )
• diabetic transgenic mice display sialadenitis

endocrine/exocrine glands
salivary gland inflammation ( J:59245 )
• diabetic transgenic mice display sialadenitis

renal/urinary system
increased urine glucose level ( J:59245 )
• seen in over 80% of mice

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
type 1 diabetes mellitus DOID:9744 OMIM:222100
 J:59245




Genotype
MGI:3623410
cx2
Allelic
Composition		 Tg(HLA-DQA1,HLA-DQB1)1Dv/0
Tg(Ins2-CD80)3B7Flv/0
Genetic
Background		 B6.Cg-Tg(HLA-DQA1,HLA-DQB1)1Dv Tg(Ins2-CD80)3B7Flv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(HLA-DQA1,HLA-DQB1)1Dv mutation (3 available)
Tg(Ins2-CD80)3B7Flv mutation (5 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased susceptibility to autoimmune diabetes ( J:59245 )
• none of the mice develop spontaneous diabetes over the 10-month observation period




Genotype
MGI:3639135
cx3
Allelic
Composition		 H2-Ab1b-tm1Gru/H2-Ab1b-tm1Gru
Tg(HLA-DQA1,HLA-DQB1)1Dv/0
Tg(Ins2-CD80)3B7Flv/0
Genetic
Background		 involves: 129S2/SvPas * C57BL/6 * C57BL/10SnJ * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2-Ab1b-tm1Gru mutation (11 available); any H2-Ab1 mutation (81 available)
Tg(HLA-DQA1,HLA-DQB1)1Dv mutation (3 available)
Tg(Ins2-CD80)3B7Flv mutation (5 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased interferon-gamma secretion ( J:68641 )
• stimulated CD4+ T cells secrete increased levels of Ifng compared to either DR4-transgenic or double transgenic MHC class II-deficient mice
decreased interleukin-6 secretion ( J:68641 )
• stimulated CD4+ T cells secrete low levels of Il-6 compared to either DR4-transgenic or double transgenic MHC class II-deficient mice
increased susceptibility to autoimmune diabetes ( J:68641 )
• 73% of mice expressing the transgene develop diabetes (blood glucose >250 mg/dl) compared to 0% of nontransgenic MHC class II-deficient mice

homeostasis/metabolism
increased circulating glucose level ( J:68641 )
• 73% display blood glucose levels above 250 mg/dl
increased urine glucose level ( J:68641 )
• seen in 73% of mice

renal/urinary system
increased urine glucose level ( J:68641 )
• seen in 73% of mice




Genotype
MGI:3639133
cx4
Allelic
Composition		 H2-Ab1b-tm1Gru/H2-Ab1b-tm1Gru
Tg(HLA-DQA1,HLA-DQB1)1Dv/0
Tg(HLA-DRB1)31Dmz/0
Tg(Ins2-CD80)3B7Flv/0
Genetic
Background		 involves: 129S2/SvPas * C57BL/6 * C57BL/10SnJ * CBA * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2-Ab1b-tm1Gru mutation (11 available); any H2-Ab1 mutation (81 available)
Tg(HLA-DQA1,HLA-DQB1)1Dv mutation (3 available)
Tg(HLA-DRB1)31Dmz mutation (1 available)
Tg(Ins2-CD80)3B7Flv mutation (5 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
salivary gland inflammation ( J:68641 )
• transgenic mice develop sialadenitis and severity is associated with diabetes development
abnormal CD4-positive, alpha beta T cell morphology ( J:68641 )
• coexpression of DR4 and DQ8 transgenes increases CD4+ T cell numbers 2-fold compared to either DR4 or DQ8 single transgenic line
abnormal CD8-positive, alpha beta T cell morphology ( J:68641 )
• there are fewer CD8+ T cells in pancreatic infiltrates than in MHC class II-deficient mice expressing only DQ8
decreased susceptibility to autoimmune diabetes ( J:68641 )
• incidence of diabetes (blood glucose >250 mg/dl; 5/22, 23%) is lowered from incidence in MHC class II-deficient mice expressing the DQ8 transgene (73%) and similar to deficient mice transgenic for DR4

endocrine/exocrine glands
salivary gland inflammation ( J:68641 )
• transgenic mice develop sialadenitis and severity is associated with diabetes development

hematopoietic system
abnormal CD4-positive, alpha beta T cell morphology ( J:68641 )
• coexpression of DR4 and DQ8 transgenes increases CD4+ T cell numbers 2-fold compared to either DR4 or DQ8 single transgenic line
abnormal CD8-positive, alpha beta T cell morphology ( J:68641 )
• there are fewer CD8+ T cells in pancreatic infiltrates than in MHC class II-deficient mice expressing only DQ8

digestive/alimentary system
salivary gland inflammation ( J:68641 )
• transgenic mice develop sialadenitis and severity is associated with diabetes development

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
type 1 diabetes mellitus DOID:9744 OMIM:222100
 J:68641




Genotype
MGI:3639140
cx5
Allelic
Composition		 H2-Ab1b-tm1Gru/H2-Ab1b-tm1Gru
Tg(Ins2-CD80)3B7Flv/0
Genetic
Background		 involves: 129S2/SvPas * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2-Ab1b-tm1Gru mutation (11 available); any H2-Ab1 mutation (81 available)
Tg(Ins2-CD80)3B7Flv mutation (5 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased susceptibility to autoimmune diabetes ( J:68641 )
• only one of the MHC class II-deficient mice developed diabetes (blood glucose >250 mg/dl)




Genotype
MGI:3639130
cx6
Allelic
Composition		 H2-Ab1b-tm1Gru/H2-Ab1b-tm1Gru
Tg(HLA-DRB1)31Dmz/0
Tg(Ins2-CD80)3B7Flv/0
Genetic
Background		 involves: 129S2/SvPas * C57BL/6 * CBA * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2-Ab1b-tm1Gru mutation (11 available); any H2-Ab1 mutation (81 available)
Tg(HLA-DRB1)31Dmz mutation (1 available)
Tg(Ins2-CD80)3B7Flv mutation (5 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
endocrine/exocrine glands
salivary gland inflammation ( J:68641 )
• transgenic mice develop sialadenitis and severity is associated with diabetes development

immune system
salivary gland inflammation ( J:68641 )
• transgenic mice develop sialadenitis and severity is associated with diabetes development
abnormal CD4-positive, alpha beta T cell morphology ( J:68641 )
• expression of the DR4 transgene increases CD4+ T cell numbers compared to the H2-Ab1-deficient Tg(Ins2-CD80)3B7Flv mice
abnormal CD8-positive, alpha beta T cell morphology ( J:68641 )
• there are fewer CD8+ T cells in pancreatic infiltrates than in MHC class II-deficient mice expressing only DQ8
decreased susceptibility to autoimmune diabetes ( J:68641 )
• incidence of diabetes (5/20) is lower than in MHC class II-deficient mice expressing the DQ8 transgene where incidence is 3-fold higher (73%)

hematopoietic system
abnormal CD4-positive, alpha beta T cell morphology ( J:68641 )
• expression of the DR4 transgene increases CD4+ T cell numbers compared to the H2-Ab1-deficient Tg(Ins2-CD80)3B7Flv mice
abnormal CD8-positive, alpha beta T cell morphology ( J:68641 )
• there are fewer CD8+ T cells in pancreatic infiltrates than in MHC class II-deficient mice expressing only DQ8

digestive/alimentary system
salivary gland inflammation ( J:68641 )
• transgenic mice develop sialadenitis and severity is associated with diabetes development

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
type 1 diabetes mellitus DOID:9744 OMIM:222100
 J:68641




Genotype
MGI:3766038
cx7
Allelic
Composition		 Tg(Ins2-CD80)3B7Flv/0
Tg(Ins2-Tnf)17Flv/0
Genetic
Background		 involves: C57BL/6 * CBA/Ca
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Ins2-CD80)3B7Flv mutation (5 available)
Tg(Ins2-Tnf)17Flv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system

homeostasis/metabolism




Genotype
MGI:3766042
cx8
Allelic
Composition		 Tg(Ins2-CD80)3B7Flv/0
Tg(Ins2-H2-Ead,Ins2-H2-Eb1b)187-7Bri/0
Genetic
Background		 involves: C57BL/6 * CBA/Ca * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Ins2-CD80)3B7Flv mutation (5 available)
Tg(Ins2-H2-Ead,Ins2-H2-Eb1b)187-7Bri mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:93555 )
• diabetes-like symptoms (dehydration, weight loss) lead to death of most animals by 6 months of age

growth/size/body
weight loss ( J:93555 )

immune system
insulitis ( J:93555 )
• mice develop insulitis starting at ~5 months of age
increased susceptibility to autoimmune diabetes ( J:93555 )
• mice develop clinical signs of diabetes

endocrine/exocrine glands
abnormal pancreatic islet morphology ( J:93555 )
• islet architecture becomes disorganized and islet tissue is replace by a ductal hyperplasia
insulitis ( J:93555 )
• mice develop insulitis starting at ~5 months of age

homeostasis/metabolism




Genotype
MGI:3757580
tg9
Allelic
Composition		 Tg(Ins2-CD80)3B7Flv/?
Genetic
Background		 involves: C57BL/6 * CBA/Ca * NOD/Caj
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Ins2-CD80)3B7Flv mutation (5 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
endocrine/exocrine glands
insulitis ( J:26618 )
• H2g7 homozygous transgenic mice show islet disruption with lymphocytic (T and B cell) infiltration, similar to diabetic NOD controls
• N2 mice after a subsequent backcross to NOD show islet disruption with lymphocytic infiltration as early as 4 weeks while non-transgenic H2g7 homozygous or heterozygous littermates show varying degrees of insulitis by ~6 weeks

immune system
insulitis ( J:26618 )
• H2g7 homozygous transgenic mice show islet disruption with lymphocytic (T and B cell) infiltration, similar to diabetic NOD controls
• N2 mice after a subsequent backcross to NOD show islet disruption with lymphocytic infiltration as early as 4 weeks while non-transgenic H2g7 homozygous or heterozygous littermates show varying degrees of insulitis by ~6 weeks
increased susceptibility to autoimmune diabetes ( J:26618 )
• 2/17 transgenic mice from the first cross to NOD develop diabetes between 10 and 14 weeks, compared to no diabetes in (NOD x C57BL/6)F1 non-transgenic controls
• after a further backcross to NOD, diabetes onset is accelerated relative to transgenic mice from the initial cross to NOD with some developing diabetes at 4 weeks; by 12 weeks, 46.2% of transgenic mice homozygous for H2g7 develop diabetes compared to no non-transgenic H2g7 homozygous littermates, or NOD controls which only start to exhibit diabetes at 12 weeks

renal/urinary system

homeostasis/metabolism
increased circulating glucose level ( J:26618 )
• transgenic mice exhibit blood glucose in excess of 13.9 mmol (250 mg/dl)

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
type 1 diabetes mellitus DOID:9744 OMIM:222100
 J:26618




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory