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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Irak4tm1Yeh
targeted mutation 1, Wen-Chen Yeh
MGI:2676448
Summary 1 genotype
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Irak4tm1Yeh/Irak4tm1Yeh involves: 129P2/OlaHsd * C57BL/6J MGI:2676450


Genotype
MGI:2676450
hm1
Allelic
Composition
Irak4tm1Yeh/Irak4tm1Yeh
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Irak4tm1Yeh mutation (1 available); any Irak4 mutation (30 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• B lymphocytes show impaired proliferation in response to LPS
• interferon gamma production by natural killer cells is undetected after challenge with lymphocytic choriomeningitis virus (LCMV), however cytolytic activity is retained
• activated macrophages are unable to produce nitric oxide in responce to IL-1
• macrophages are unable to produce inflammatory mediators in response to treatment with either peptidoglycan or poly(I:C)
• bone-marrow derived macrophages are unresponsive to LPS stimulation and do not produce cytokines
• mutants show no cytokine response to LPS
• serum IFN-gamma levels are undetectable after lymphocytic chriomeningitis virus infection
• decreased serum IL-1 levels after LPS-induced septic shock
• no response to IL-1 by production of IL-6, Tnf, or nitric oxide, or by activation of Nfkb or Jnk
• responses to Tnf, however, were intact, suggesting that the defect was specific for IL-1
• decreased serum IL-6 levels after LPS-induced septic shock or IL-1beta injection
• decreased serum TNF-alpha levels after LPS-induced septic shock or IL-1beta injection
• mutants show no cytokine response to LPS
• mouse embryonic fibroblasts treated with IL-1 show defects in IL-6 production
• bacterial DNA is unable to stimulate IL-6 production in mutants
• mouse embryonic fibroblasts treated with IL-1 show defects in TNF-alpha production
• complete resistance to a lethal dose of LPS
• whereas 80% of wild-type control mice survived for 10 days after inoculation with Staphylococcus aureus, all inoculated mutant mice die by day 10

homeostasis/metabolism
• mutants show no cytokine response to LPS
• serum IFN-gamma levels are undetectable after lymphocytic chriomeningitis virus infection
• decreased serum IL-1 levels after LPS-induced septic shock
• no response to IL-1 by production of IL-6, Tnf, or nitric oxide, or by activation of Nfkb or Jnk
• responses to Tnf, however, were intact, suggesting that the defect was specific for IL-1
• decreased serum IL-6 levels after LPS-induced septic shock or IL-1beta injection
• decreased serum TNF-alpha levels after LPS-induced septic shock or IL-1beta injection

hematopoietic system
• B lymphocytes show impaired proliferation in response to LPS
• interferon gamma production by natural killer cells is undetected after challenge with lymphocytic choriomeningitis virus (LCMV), however cytolytic activity is retained
• activated macrophages are unable to produce nitric oxide in responce to IL-1
• macrophages are unable to produce inflammatory mediators in response to treatment with either peptidoglycan or poly(I:C)
• bone-marrow derived macrophages are unresponsive to LPS stimulation and do not produce cytokines

cellular
• B lymphocytes show impaired proliferation in response to LPS





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory