All Phenotypes Prkg1<tm2.1Naw> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prkg1^tm2.1Naw/Prkg1^tm2.1Naw	involves: 129S1/Sv * 129X1/SvJ	MGI:3797266
cn2	Neurod6^tm1(cre)Kan/Neurod6⁺ Prkg1^tm2Naw/Prkg1^tm2.1Naw	involves: 129S1/Sv * 129X1/SvJ	MGI:2668663
cx3	Prkg1^tm2.1Naw/Prkg1^tm2.1Naw Tagln^{tm1(PRKG1*)Hfm}/Tagln⁺	involves: 129S1/Sv * 129X1/SvJ	MGI:3797264
cx4	Prkg1^tm2.1Naw/Prkg1^tm2.1Naw Tagln^{tm2(PRKG1*)Hfm}/Tagln⁺	involves: 129S1/Sv * 129X1/SvJ	MGI:3797265

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:134928 )

• mice have a median survival time of under 6 weeks

hematopoietic system

enlarged spleen ( J:134639 )

• splenomegaly first occurs between 5 and 10 weeks of age

• spleens are about 3-fold larger based upon organ/bodyweight ratio

• splenomegaly is evident in most 8 to 10 week old mice splenomegaly is evident in most 8 to 10 week old mice

anemia ( J:134639 )

• mice are anemic by 3-4 weeks of age, which is before the first signs of enlarged spleens

abnormal erythrocyte morphology ( J:134639 )

• red blood cells have about 2-fold higher levels of apoptosis when incubated in Ringer's solution

• red blood cells are also slightly but significantly more flexible in mechanical stress tests

decreased erythrocyte cell number ( J:134639 )

• there is a 52% decrease in the number of red blood cells

decreased hematocrit ( J:134639 )

• hematocrit is significantly decreased

increased mean corpuscular hemoglobin ( J:134639 )

• mice have a significant increase in their mean corpuscular hemoglobin content

increased mean corpuscular volume ( J:134639 )

• there is about a 40% increase in the MCV

increased red blood cell distribution width ( J:134639 )

• the red blood cell distribution width is increased

reticulocytosis ( J:134639 )

• the reticulocyte number is higher in these mice than in control mice

increased erythrocyte clearance ( J:134639 )

• red blood cells are cleared from circulation almost 3-fold faster than in control mice

digestive/alimentary system

abnormal intestinal transit time ( J:134928 )

• intestinal transit time is significantly prolonged in these mice

cardiovascular system

increased mean systemic arterial blood pressure ( J:134928 )

• the mean arterial blood pressure of conscious mice is 11.5% higher than in control mice

• blood pressure is also elevated in unconscious mice and is unresponsive to nitrous oxide realizing drugs

abnormal vascular smooth muscle physiology ( J:134928 )

• cGMP fails to reduce norepinephrine-induced or potassium-induced calcium transients in vascular smooth muscle cells

muscle

abnormal vascular smooth muscle physiology ( J:134928 )

• cGMP fails to reduce norepinephrine-induced or potassium-induced calcium transients in vascular smooth muscle cells

homeostasis/metabolism

abnormal circulating hormone level ( J:134639 )

• there is about a 2-fold increase in erythropoietin plasma levels

immune system

enlarged spleen ( J:134639 )

• splenomegaly first occurs between 5 and 10 weeks of age

• spleens are about 3-fold larger based upon organ/bodyweight ratio

• splenomegaly is evident in most 8 to 10 week old mice splenomegaly is evident in most 8 to 10 week old mice

growth/size/body

enlarged spleen ( J:134639 )

• splenomegaly first occurs between 5 and 10 weeks of age

• spleens are about 3-fold larger based upon organ/bodyweight ratio

• splenomegaly is evident in most 8 to 10 week old mice splenomegaly is evident in most 8 to 10 week old mice

Allelic Composition	Neurod6^tm1(cre)Kan/Neurod6⁺ Prkg1^tm2Naw/Prkg1^tm2.1Naw
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Neurod6^tm1(cre)Kan mutation (0 available); any Neurod6 mutation (17 available)
Prkg1^tm2.1Naw mutation (0 available); any Prkg1 mutation (58 available)
Prkg1^tm2Naw mutation (0 available); any Prkg1 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:84444 )

N	• unlike Prkg1^tm1Hfm homozygotes, conditional mutant mice have a normal life expectancy

nervous system

nervous system phenotype ( J:84444 )

N	• adult conditional mutant mice display no significant differences in baseline synaptic transmission relative to control littermates

reduced long-term potentiation ( J:84444 )

• adult (12-14 weeks of age) but not juvenile (3-4 weeks of age) conditional mutant mice display reduced hippocampal LTP after repetitive episodes of theta burst stimulation (TBS)

• the difference in LTP between adult conditional and control mice is abolished by anisomycin (a protein synthesis inhibitor), suggesting a defect in late-phase LTP

• however, both juvenile and adult conditional mutant mice show a normal LTP in response to a single episode of tetanic stimulation, regardless of whether a weak TBS or a strong tetanic stimulus is used

behavior/neurological

behavior/neurological phenotype ( J:84444 )

• despite a deficit in late-phase LTP, adult conditional mutant mice exhibit normal performance in hippocampus-dependent behavioral tests, i.e., contextual fear conditioning and spatial learning, with no significant differences in the freezing response to the conditioning context, in acquisition of a spatial searching strategy, or in storage and retrieval of spatial memory relative to control mice

cardiovascular system

cardiovascular system phenotype ( J:84444 )

N	• unlike Prkg1^tm1Hfm homozygotes, conditional mutant mice display no detectable cardiovascular abnormalities

digestive/alimentary system

digestive/alimentary phenotype ( J:84444 )

N	• unlike Prkg1^tm1Hfm homozygotes, conditional mutant mice display no detectable gastrointestinal abnormalities

Allelic Composition	Prkg1^tm2.1Naw/Prkg1^tm2.1Naw Tagln^{tm1(PRKG1*)Hfm}/Tagln⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prkg1^tm2.1Naw mutation (0 available); any Prkg1 mutation (58 available)
Tagln^{tm1(PRKG1*)Hfm} mutation (0 available); any Tagln mutation (26 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:134928 )

• mice have a median survival time of 52 weeks

• this survival time is significantly longer than Prkg1^tm2.1Naw homozygote that do not carry the knock-in allele

reproductive system

reduced female fertility ( J:134928 )

• female mice have a reduced success rate of pregnancy

decreased litter size ( J:134928 )

• mice have slightly reduced litter size of 3 to 6 pups

Allelic Composition	Prkg1^tm2.1Naw/Prkg1^tm2.1Naw Tagln^{tm2(PRKG1*)Hfm}/Tagln⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prkg1^tm2.1Naw mutation (0 available); any Prkg1 mutation (58 available)
Tagln^{tm2(PRKG1*)Hfm} mutation (0 available); any Tagln mutation (26 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:134928 )

• mice have a median survival time of 52 weeks

• this survival time is significantly longer than Prkg1^tm2.1Naw homozygote that do not carry the knock-in allele

reproductive system

reduced female fertility ( J:134928 )

• female mice have a reduced success rate of pregnancy

decreased litter size ( J:134928 )

• mice have slightly reduced litter size of 3 to 6 pups

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