Phenotypes associated with this allele

• Allele Symbol: Gnb5^tm1Ck
• Allele Name: targeted mutation 1, Ching-Kang Chen
• Allele ID: MGI:2664296
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gnb5^tm1Ck/Gnb5^tm1Ck	either: (involves: 129S/SvEv * 129S1/Sv) or (involves: 129S/SvEv * 129S1/Sv * C57BL/6)	MGI:2664297
hm2	Gnb5^tm1Ck/Gnb5^tm1Ck	involves: 129S1/Sv	MGI:5301444

Genotype
MGI:2664297

hm1

• Allelic Composition: Gnb5^tm1Ck/Gnb5^tm1Ck
• Genetic Background: either: (involves: 129S/SvEv * 129S1/Sv) or (involves: 129S/SvEv * 129S1/Sv * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, incomplete penetrance ( J:83620 )

• >66% of homozygotes die before weaning, mostly between P15 and P20

• preweaning mortality can be bypassed by initiating hand-feeding at P2 with homozygotes surviving to adulthood

growth/size/body

decreased body size ( J:83620 )

• mutant pups appear runted relative to wild-type pups

slow postnatal weight gain ( J:83620 )

• homozygotes exhibit a slower weight gain in the first month of life relative to wild-type controls

• a period of no weight gain is observed from P15 to P20

nervous system

nervous system phenotype ( J:83620 )

N • surprisingly, surviving homozygotes display no gross defects in brain morphology at 3 months of age

vision/eye

vision/eye phenotype ( J:83620 )

N • surprisingly, surviving homozygotes display no gross defects in retina morphology at 3 months of age

Genotype
MGI:5301444

hm2

• Allelic Composition: Gnb5^tm1Ck/Gnb5^tm1Ck
• Genetic Background: involves: 129S1/Sv

cardiovascular system

abnormal sinoatrial node conduction ( J:178182 )

• carbachol currents show prolonged deactivation kinetics