Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mpltm1Wsa mutation
(1 available);
any
Mpl mutation
(46 available)
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hematopoietic system
homeostasis/metabolism
immune system
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• plasma levels of CXCL4, CXCL5, and CXCL7 are attenuated compared to in wild-type mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mpltm1Wsa mutation
(1 available);
any
Mpl mutation
(46 available)
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hematopoietic system
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• mutant mice displayed a loss of megakaryocytes in the spleen and bone marrow
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• mice are deficient in megakaryocyte progenitor cells
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• mice had only 6% the number platelets detected in controls
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• in clonal culture of bone marrow, total progenotor cell number was 38%- 44% of wildtype
• neutrophil-CFC, neutrophil-macrophage-CFC and pure erythroid-CFC were present at approximately 50% of normal levels
• cell reduction not seen in mature cell populations
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immune system
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N |
• no alterations were detected in the ratios of B and T cells examined in the spleen, thymus and bone marrow using momoclonal antibodies
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immune system
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• WBC is reduced by more than a third compared to Mpltm1Wsa homozygote controls
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hematopoietic system
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• there is a slight reduction in the number of red blood cells compared to Mpltm1Wsa homozygote controls
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• platelet count is reduced by about half compared to Mpltm1Wsa homozygote controls
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• WBC is reduced by more than a third compared to Mpltm1Wsa homozygote controls
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ergmld2 mutation
(0 available);
any
Erg mutation
(27 available)
Mpltm1Wsa mutation
(1 available);
any
Mpl mutation
(46 available)
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hematopoietic system
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• platelet counts are significantly lower than single Mpl-deficient animals
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• at lower end of normal range
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immune system
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• at lower end of normal range
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mpltm1Wsa mutation
(1 available);
any
Mpl mutation
(46 available)
Suz12Plt8 mutation
(0 available);
any
Suz12 mutation
(60 available)
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hematopoietic system
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• amelioration of thrombocytopenia compared to Mpltm1Wsa , increased platelet cell number was significant but not to the wild-type level
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• hematopoietic stem cell number were increased in the bone marrow compared to Mpltm1Wsa control, but not to the wild-type level
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• megakaryocyte numbers were increased in the bone marrow compared to Mpltm1Wsa control, but not to the wild-type level
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• thrombocytopenia of Mpltm1Wsa ameliorated, however platelet count is significantly lower than normal wild-type level
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immune system
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• amelioration of thrombocytopenia compared to Mpltm1Wsa , increased platelet cell number was significant but not to the wild-type level
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cellular
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• megakaryocyte numbers were increased in the bone marrow compared to Mpltm1Wsa control, but not to the wild-type level
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mpltm1Wsa mutation
(1 available);
any
Mpl mutation
(46 available)
Suz12Plt8 mutation
(0 available);
any
Suz12 mutation
(60 available)
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mortality/aging
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• no homozygous Suz12Plt8Hiltanimals were found, indicating prenatal lethality
• embryos homozygous for Suz12Plt8Hilt were reduced in number at embryonic day 8.5
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embryo
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• embryos homozygous for Suz12Plt8Hilt were smaller than wild-type
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growth/size/body
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• embryos homozygous for Suz12Plt8Hilt were smaller than wild-type
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hematopoietic system
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• amelioration of thrombocytopenia compared to Mpltm1Wsa , increased platelet cell number was significant but not to the wild-type level
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• thrombocytopenia of Mpltm1Wsa ameliorated, however platelet count is significantly lower than normal wild-type level
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immune system
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• amelioration of thrombocytopenia compared to Mpltm1Wsa , increased platelet cell number was significant but not to the wild-type level
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hematopoietic system
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N |
• mice do not show leukopenia or anemia
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• platelet counts are lower than single Mpl-deficient littermates at 7 weeks of age
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mpltm1Wsa mutation
(1 available);
any
Mpl mutation
(46 available)
MybPlt3 mutation
(0 available);
any
Myb mutation
(53 available)
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mortality/aging
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• lower than expected numbers of homozygotes born in matings of heterozygotes indicating a degree of perinatal or embryonic lethality
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hematopoietic system
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• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
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• numbers of most progenitor cell lines are elevated
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• defective production of erythroid lineages
• large numbers of erythroid colony forming units but defective maturation
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• leukopenic
• significantly reduced numbers of B cells in bone marrow and spleen
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• supraphysiological levels
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• expanded red pulp with abnormal architecture
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• abnormally small lymphoid follicles
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immune system
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• leukopenic
• significantly reduced numbers of B cells in bone marrow and spleen
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• expanded red pulp with abnormal architecture
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• abnormally small lymphoid follicles
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cellular
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• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mpltm1Wsa mutation
(1 available);
any
Mpl mutation
(46 available)
MybPlt3 mutation
(0 available);
any
Myb mutation
(53 available)
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hematopoietic system
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• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
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• numbers of most progenitor cell lines are unchanged
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• amelioration of thrombocytopenia compared to Mpltm1Wsa but not to wild-type
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cellular
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• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
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immune system
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mpltm1Wsa mutation
(1 available);
any
Mpl mutation
(46 available)
MybPlt4 mutation
(0 available);
any
Myb mutation
(53 available)
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hematopoietic system
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• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
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• numbers of most progenitor cell lines are elevated
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• defective production of erythroid lineages
• large numbers of erythroid colony forming units but defective maturation
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• supraphysiological levels
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• leukopenic
• significantly reduced numbers of B cells in bone marrow and spleen
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• expanded red pulp with abnormal architecture
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• abnormally small lymphoid follicles
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immune system
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• leukopenic
• significantly reduced numbers of B cells in bone marrow and spleen
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• expanded red pulp with abnormal architecture
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• abnormally small lymphoid follicles
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cellular
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• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mpltm1Wsa mutation
(1 available);
any
Mpl mutation
(46 available)
MybPlt4 mutation
(0 available);
any
Myb mutation
(53 available)
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hematopoietic system
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• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
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• numbers of most progenitor cell lines are unchanged
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• amelioration of thrombocytopenia compared to Mpltm1Wsa but not to wild-type
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cellular
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• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
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immune system
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ep300Plt6 mutation
(0 available);
any
Ep300 mutation
(97 available)
Mpltm1Wsa mutation
(1 available);
any
Mpl mutation
(46 available)
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mortality/aging
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• about 80% of mice fail to survive to weaning
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hematopoietic system
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• megakarycoyte numbers are about 10-fold higher in the bone marrow and spleen
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• clonogenic myeloid progenitor cells are also significantly elevated by more than 3-fold in the spleen
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• platelet count is increased more than 10-fold over Mpltm1Wsa homozygotes and more than twice as much as wild-type mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ep300Plt6 mutation
(0 available);
any
Ep300 mutation
(97 available)
Mpltm1Wsa mutation
(1 available);
any
Mpl mutation
(46 available)
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immune system
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• the eosinophil number is almost double that of Mpltm1Wsa homozygotes and is similar to wild-type mice
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hematopoietic system
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• megakaryocyte numbers in the bone marrow and spleen are more than double what is found of wild-type mice
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• clonogenic myeloid progenitor cells are also significantly elevated by more than 3-fold in the spleen
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• the eosinophil number is almost double that of Mpltm1Wsa homozygotes and is similar to wild-type mice
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• platelet count is increased more than 2-fold compared to Mpltm1Wsa homozygotes but is about half the number found in wild-type mice
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Analysis Tools