Phenotypes associated with this allele

• Allele Symbol: Bub1⁺
• Allele Name: wild type
• Allele ID: MGI:2434961
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Bub1^tm1.1Tvd/Bub1^+	involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6J	MGI:3830990
ht2	Bub1^Gt(XG453)Byg/Bub1^+	involves: 129P2/OlaHsd * C57BL/6J	MGI:3830994
ht3	Bub1^tm1Tvd/Bub1^+	involves: 129P2/OlaHsd * C57BL/6J	MGI:3830989
ht4	Bub1^tm1.1Jvd/Bub1^+	involves: 129/Sv * C57BL/6	MGI:3805689
ht5	Bub1^tm2Jvd/Bub1^+	involves: 129/Sv * C57BL/6	MGI:3805690

Genotype
MGI:3830990

ht1

• Allelic Composition: Bub1^tm1.1Tvd/Bub1⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

aneuploidy ( J:143035 )

• 23% of mouse embryonic fibroblasts exhibit aneuploidy compared to 9% of wild-type cells

neoplasm

increased brain tumor incidence ( J:143035 )

• mice exhibit brain tumors unlike wild-type mice

increased lung tumor incidence ( J:143035 )

• mice exhibit lung tumors unlike wild-type mice

nervous system

increased brain tumor incidence ( J:143035 )

• mice exhibit brain tumors unlike wild-type mice

respiratory system

increased lung tumor incidence ( J:143035 )

• mice exhibit lung tumors unlike wild-type mice

Genotype
MGI:3830994

ht2

• Allelic Composition: Bub1^Gt(XG453)Byg/Bub1⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

cellular

maternal effect ( J:143035 )

• 90% of mice of all genotypes are resorbed by E14.

Genotype
MGI:3830989

ht3

• Allelic Composition: Bub1^tm1Tvd/Bub1⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

normal phenotype

no abnormal phenotype detected ( J:143035 )

• mice are viable and fertile

Genotype
MGI:3805689

ht4

• Allelic Composition: Bub1^tm1.1Jvd/Bub1⁺
• Genetic Background: involves: 129/Sv * C57BL/6

cellular

aneuploidy ( J:135093 )

• at 5 months, metaphase spreads from splenocytes exhibit 16% incidence of aneuploidy

• aneuploid metaphases in MEFs is higher than in wild-type but lower than observed in Bub1^tm1.1Jvd heterozygotes or Bub1^tm2vd heterozygotes

abnormal cell cycle checkpoint function ( J:135093 )

• MEFs treated with nocadozole to induce cycle arrest are impaired in ability to maintain arrest, with 50% of cells exiting mitosis around 5.4 hours compared to arrest in prometaphase for 7.2 hours in wild-type MEFs

abnormal mitosis ( J:135093 )

• 14% of mitotic figures from splenocytes show premature separation of sister chromatids

• in MEFs chromosome segregation defects resulting from chromosome lagging or congression failure are observed at much higher rates than in wild-type MEFs; most events involve a single chromosome but some involve two or three chromosomes

decreased fibroblast apoptosis ( J:135093 )

• MEFs in which chromosome missegregation during mitosis occurs show a apoptotic rate of 65% compared to apoptosis of 95% of wild-type cells with such events

neoplasm

increased incidence of tumors by chemical induction ( J:135093 )

• injection of DMBA into dorsal skin of pups at 3-5 days results exclusively in lung tumors with a 2-3-fold higher incidence than in wild-type; tumor burden is increased around 3-fold compared to wild-type

decreased tumor incidence ( J:135093 )

• mice show trend toward decreased spontaneous tumor formation particularly in liver and lung tissue

homeostasis/metabolism

increased incidence of tumors by chemical induction ( J:135093 )

• injection of DMBA into dorsal skin of pups at 3-5 days results exclusively in lung tumors with a 2-3-fold higher incidence than in wild-type; tumor burden is increased around 3-fold compared to wild-type

Genotype
MGI:3805690

ht5

• Allelic Composition: Bub1^tm2Jvd/Bub1⁺
• Genetic Background: involves: 129/Sv * C57BL/6

cellular

aneuploidy ( J:135093 )

• at 5 months, metaphase spreads from splenocytes exhibit 6% incidence of aneuploidy

• aneuploid metaphases in MEFs is higher than in wild-type but lower than observed in Bub1^tm1.1Jvd heterozygotes or Bub1^tm2vd heterozygotes

abnormal cell cycle checkpoint function ( J:135093 )

• MEFs treated with nocadozole to induce cycle arrest are impaired in ability to maintain arrest, with 50% of cells exiting mitosis around 5.4 hours compared to arrest in prometaphase for 7.2 hours in wild-type MEFs

abnormal mitosis ( J:135093 )

• in MEFs chromosome segregation defects resulting from chromosome lagging or congression failure are observed at much higher rates than in wild-type MEFs; most events involve a single chromosome but some involve two or three chromosomes

decreased fibroblast apoptosis ( J:135093 )

• MEFs in which chromosome missegregation during mitosis occurs show a apoptotic rate of 68% compared to apoptosis of 95% of wild-type cells with such events