All Phenotypes Gpx4<+> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Gpx4^tm1Ssd/Gpx4⁺	B6.129-Gpx4^tm1Ssd	MGI:3796141
ht2	Gpx4^{tm1.1(KOMP)Vlcg}/Gpx4⁺	C57BL/6N-Gpx4^{tm1.1(KOMP)Vlcg}/MbpMmucd	MGI:6325144
ht3	Gpx4^tm1Prol/Gpx4⁺	involves: 129	MGI:2651598
ht4	Gpx4^tm1Prol/Gpx4⁺	involves: 129 * C57BL/6J	MGI:2651593
ht5	Gpx4^tm4Marc/Gpx4⁺	involves: 129S6/SvEvTac * C57BL/6J	MGI:5803864
cx6	Gpx4^tm5Marc/Gpx4⁺ Sepsecs^tm1.1Usch/Sepsecs^tm1.1Usch	involves: 129S6/SvEvTac * C57BL/6J	MGI:7367143

Allelic Composition	Gpx4^tm1Ssd/Gpx4⁺
Genetic Background	B6.129-Gpx4^tm1Ssd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpx4^tm1Ssd mutation (0 available); any Gpx4 mutation (80 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cellular

abnormal mitochondrial physiology ( J:134214 )

• lung fibroblasts have a lower mitochondrial membrane potential, lower levels of reduced thiols, and lower overall levels of cardiolipins

• thiol and cardiolipin oxidation that results from chemical agents causing oxidative stress is reduced in mitochondria of lung fibroblasts

oxidative stress ( J:134214 )

• fibroblasts from lung have increased cytotoxicity to chemicals that cause oxidative stress including low doses of hydrogen peroxide (25 micromolar) and cadmium (concentrations between 75 and 200 micromolar)

• however, fibroblasts have decreased cytotoxicity to phosphatidylcholine hydroperoxide

Allelic Composition	Gpx4^{tm1.1(KOMP)Vlcg}/Gpx4⁺
Genetic Background	C57BL/6N-Gpx4^{tm1.1(KOMP)Vlcg}/MbpMmucd
Cell Lines	13536A-B6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpx4^{tm1.1(KOMP)Vlcg} mutation (1 available); any Gpx4 mutation (80 available)

Data Sources

IMPC Data for Gpx4

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

hydrometra ( J:211773 )

IMPC - UCD

Allelic Composition	Gpx4^tm1Prol/Gpx4⁺
Genetic Background	involves: 129

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpx4^tm1Prol mutation (1 available); any Gpx4 mutation (80 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

normal phenotype

no abnormal phenotype detected ( J:82053 )

• Background Sensitivity: gamma-irradiation did not affect survival of mice on a 129/Sv genetic background

Allelic Composition	Gpx4^tm1Prol/Gpx4⁺
Genetic Background	involves: 129 * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpx4^tm1Prol mutation (1 available); any Gpx4 mutation (80 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

increased mortality induced by gamma-irradiation ( J:82053 )

• reduced survival on mixed 129/Sv and C57BL/6 genetic background relative to control littermates

homeostasis/metabolism

increased mortality induced by gamma-irradiation ( J:82053 )

• reduced survival on mixed 129/Sv and C57BL/6 genetic background relative to control littermates

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Severe morphological alterations in the midpiece of developing and mature sperm in Gpx4^tm4Marc/Gpx4⁺ mice

reproductive system

abnormal sperm midpiece morphology ( J:225651 )

• immunocytochemical staining of whole-mount spermatozoa revealed elevated Gpx4 expression particularly in the midpiece of isolated spermatozoa

• many severe morphological defecs are observed in the midpiece and midpiece-principal piece junction of developing and mature sperm

• aberrations include bends mainly at the midpiece-principal piece junction, irregularly aligned mitochondria, and protrusions of outer dense fibers

increased sperm mitochondrial size ( J:225651 )

• late stage spermatids and epididymal spermatozoa exhibit swollen mitochondria surrounding the axoneme

kinked sperm flagellum ( J:225651 )

• bends mainly at the midpiece-principal piece junction

abnormal sperm head morphology ( J:225651 )

• immunocytochemical staining of whole-mount spermatozoa revealed elevated Gpx4 expression in the head of isolated spermatozoa

decreased litter size ( J:225651 )

• heterozygous males yield litter sizes ranging from 2 to 10 pups per litter relative to 1 to 11 pups per litter in wild-type controls

reduced male fertility ( J:225651 )

• male heterozygotes are subfertile in conventional breeding and in vitro fertilization assays

• test breeding of heterozygous males with wild-type C57BL/6J females yielded 11 litters with a total number of 70 offspring whereas breeding of wild-type males yielded 17 litters with a total number of 119 offspring

impaired fertilization ( J:225651 )

• in vitro fertilization assays using heterozygous mutant sperm and wild-type oocytes revealed a significant reduction in the number of zygotes (pro-nuclei stage embryos) and developed blastocysts (16 zygotes; 2 blastocysts) relative to wild-type controls (105 zygotes; 20 blastocysts)

decreased fertilization frequency ( J:225651 )

abnormal sperm physiology ( J:225651 )

• sperm isolated from the cauda epididymis exhibit higher levels of free reduced thiols than wild-type sperm

abnormal sperm motility ( J:225651 )

• spermatozoa from heterozygous males exhibit a decrease in overall sperm progressivity

• however, total sperm concentration and motility are normal

cellular

abnormal sperm midpiece morphology ( J:225651 )

• immunocytochemical staining of whole-mount spermatozoa revealed elevated Gpx4 expression particularly in the midpiece of isolated spermatozoa

• many severe morphological defecs are observed in the midpiece and midpiece-principal piece junction of developing and mature sperm

• aberrations include bends mainly at the midpiece-principal piece junction, irregularly aligned mitochondria, and protrusions of outer dense fibers

increased sperm mitochondrial size ( J:225651 )

• late stage spermatids and epididymal spermatozoa exhibit swollen mitochondria surrounding the axoneme

kinked sperm flagellum ( J:225651 )

• bends mainly at the midpiece-principal piece junction

abnormal sperm head morphology ( J:225651 )

• immunocytochemical staining of whole-mount spermatozoa revealed elevated Gpx4 expression in the head of isolated spermatozoa

abnormal sperm motility ( J:225651 )

• spermatozoa from heterozygous males exhibit a decrease in overall sperm progressivity

• however, total sperm concentration and motility are normal

Allelic Composition	Gpx4^tm5Marc/Gpx4⁺ Sepsecs^tm1.1Usch/Sepsecs^tm1.1Usch
Genetic Background	involves: 129S6/SvEvTac * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpx4^tm5Marc mutation (0 available); any Gpx4 mutation (80 available)
Sepsecs^tm1.1Usch mutation (0 available); any Sepsecs mutation (25 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:315052 )

N	• mice exhibit normal survival

cardiovascular system

decreased heart rate ( J:315052 )

• slightly decreased

respiratory system

respiratory system phenotype ( J:315052 )

N	• mice exhibit normal breathing frequency

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