Phenotypes associated with this allele

• Allele Symbol: Dlk1⁺
• Allele Name: wild type
• Allele ID: MGI:2430410
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Dlk1^tm1Hsul/Dlk1^+	involves: 129 * C57BL/6J	MGI:3641423
ht2	Dlk1^tm1.2Jvs/Dlk1^+	involves: 129P2/OlaHsd * C57BL/6 * FVB/N	MGI:5471941
ht3	Dlk1^tm1Srba/Dlk1^+	involves: 129X1/SvJ * C57BL/6	MGI:3838138
cn4	Dlk1^tm1.1Jvs/Dlk1^+ Myf5^tm3(cre)Sor/Myf5^+	involves: 129P2/SvPas * 129S4/SvJaeSor * C57BL/6 * FVB/N	MGI:4879116
cn5	Dlk1^tm1.1Jvs/Dlk1^+ Tg(Tek-cre)12Flv/0	involves: 129P2/OlaHsd * C3H * C57BL/6 * FVB/N	MGI:5471939
cn6	Dlk1^tm1.1Jvs/Dlk1^+ Tg(Ins2-cre)25Mgn/0	involves: 129P2/OlaHsd * C57BL/6 * DBA * FVB/N	MGI:5471938
cn7	Dlk1^tm1.1Jvs/Dlk1^+ Tg(Gh1-cre)bKnmn/0	involves: 129P2/OlaHsd * C57BL/6 * FVB/N	MGI:5471937

Genotype
MGI:3641423

ht1

• Allelic Composition: Dlk1^tm1Hsul/Dlk1⁺
• Genetic Background: involves: 129 * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

postnatal growth retardation ( J:77909 )

• growth retardation with moderately larger fat pad mass in paternally inherited mutant heterozygous mice at 21 and 56 days of age

adipose tissue

abnormal fat pad morphology ( J:77909 )

• increased in white fat pad mass in paternally inherited mutant heterozygous mice when fed a high fat diet

cellular

maternal imprinting ( J:77909 )

• Dlk1 is expressed only from the paternal allele

Genotype
MGI:5471941

ht2

• Allelic Composition: Dlk1^tm1.2Jvs/Dlk1⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * FVB/N

mortality/aging

mortality/aging ( J:194130 )

N • when the allele is inherited maternally, mice exhibit normal survival

neonatal lethality ( J:194130 )

• when the allele is inherited paternally, some mice die within the first few days after birth

postnatal lethality ( J:194130 )

• when the allele is inherited paternally, some mice die within the first few days after birth

embryo

decreased trophoblast glycogen cell number ( J:194130 )

• decreased number of glycogen cells with an increase in pre-glycogen cells, when the allele is inherited paternally

abnormal placenta labyrinth morphology ( J:194130 )

• increased in cellularity, when the allele is inherited paternally

abnormal placental labyrinth vasculature morphology ( J:194130 )

• decreased vascularity when the allele is inherited paternally

placental labyrinth hypoplasia ( J:194130 )

• reduced size of labyrinth layer when the allele is inherited paternally

growth/size/body

growth/size/body region phenotype ( J:194130 )

N • when the allele is inherited paternally, mice exhibit normal embryonic weight

N • when the allele is inherited maternally, mice exhibit normal growth

decreased body size ( J:194130 )

• dwarfism when the allele is inherited paternally

decreased body weight ( J:194130 )

• from 1 to 6 weeks when the allele is inherited paternally

postnatal growth retardation ( J:194130 )

• when the allele is inherited paternally

adipose tissue

decreased fat cell size ( J:194130 )

• when the allele is inherited paternally at 6, but not 16, weeks

cardiovascular system

abnormal placental labyrinth vasculature morphology ( J:194130 )

• decreased vascularity when the allele is inherited paternally

Genotype
MGI:3838138

ht3

• Allelic Composition: Dlk1^tm1Srba/Dlk1⁺
• Genetic Background: involves: 129X1/SvJ * C57BL/6

cellular

maternal imprinting ( J:145076 )

• locus is maternally imprinted with no gene expression detectable in neonatal liver of mice that have paternally inherited the mutant allele

Genotype
MGI:4879116

cn4

• Allelic Composition: Dlk1^tm1.1Jvs/Dlk1⁺; Myf5^tm3(cre)Sor/Myf5⁺
• Genetic Background: involves: 129P2/SvPas * 129S4/SvJaeSor * C57BL/6 * FVB/N

muscle

increased skeletal muscle fiber size ( J:167124 )

• increased average size of the myotubes formed in satellite cell-derived primary myoblasts over-expressing Dlk1 when the allele is inherited paternally

increased skeletal muscle fiber diameter ( J:167124 )

• increased diameters of the resulting myotubes in the presence of Dlk1 coated substrate when the allele is inherited paternally

decreased skeletal muscle fiber number ( J:167124 )

• 25% reduction in total myofiber numbers in fast (EDL) and slow (soleus) muscles when the allele is inherited paternally

skeletal muscle fibrosis ( J:167124 )

• extensive fibrosis, scarification, and interstitial space occupied by massive cellular infiltration at 5-7 days after intramuscular injections of CTX to the tibialis anterior (TA) muscles when the allele is inherited paternally

impaired skeletal muscle regeneration ( J:167124 )

• impaired regeneration at 5-7 days after intramuscular injections of CTX to the tibialis anterior (TA) muscles when the allele is inherited paternally

• roughly 25% decrease in nascent de novo fiber formation five days after injury when the allele is inherited paternally

• extensive fibrosis, scarification, and interstitial space occupied by massive cellular infiltration when the allele is inherited paternally

cellular

abnormal cell differentiation ( J:167124 )

• increased proportion of self-renewing cells (Pax7+/MyoD-) in the cultured myoblasts on the mutant EDL fiber when the allele is inherited paternally

• accelerated differentiation kinetics in satellite cell-derived primary myoblasts over-expressing Dlk1 or in the presence of Dlk1 coated substrate when the allele is inherited paternally

decreased cell proliferation ( J:167124 )

• decreased proliferating cells (Pax7+/ MyoD+) in the cultured primary myoblasts on the mutant EDL fiber when the allele is inherited paternally

• reduced cell numbers at day 3 after transfection in satellite cell-derived primary myoblasts over-expressing Dlk1 when the allele is inherited paternally

• reduced numbers of proliferating cells (Ki67+) in satellite cell-derived primary myoblasts over-expressing Dlk1 or in the presence of Dlk1 coated substrate when the allele is inherited paternally

maternal imprinting ( J:167124 )

• Dlk1 is an imprinted gene expressed only from paternal allele

growth/size/body

decreased body weight ( J:167124 )

• reduced body weight when the allele is inherited paternally

adipose tissue

decreased brown adipose tissue mass ( J:167124 )

• decreased brown fat mass by 20% when the allele is inherited paternally

immune system

increased inflammatory response ( J:167124 )

• increased interstitial nuclei density- excessive infiltrated macrophages after injury when the allele is inherited paternally

Genotype
MGI:5471939

cn5

• Allelic Composition: Dlk1^tm1.1Jvs/Dlk1⁺; Tg(Tek-cre)12Flv/0
• Genetic Background: involves: 129P2/OlaHsd * C3H * C57BL/6 * FVB/N

mortality/aging

mortality/aging ( J:194130 )

N • mice exhibit normal survival

growth/size/body

growth/size/body region phenotype ( J:194130 )

N • mice exhibit normal growth

Genotype
MGI:5471938

cn6

• Allelic Composition: Dlk1^tm1.1Jvs/Dlk1⁺; Tg(Ins2-cre)25Mgn/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * DBA * FVB/N

mortality/aging

mortality/aging ( J:194130 )

N • mice exhibit normal survival

growth/size/body

growth/size/body region phenotype ( J:194130 )

N • mice exhibit normal growth

Genotype
MGI:5471937

cn7

• Allelic Composition: Dlk1^tm1.1Jvs/Dlk1⁺; Tg(Gh1-cre)bKnmn/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * FVB/N

mortality/aging

mortality/aging ( J:194130 )

N • mice exhibit normal survival

growth/size/body

growth/size/body region phenotype ( J:194130 )

N • mice exhibit normal growth