Phenotypes associated with this allele

• Allele Symbol: Ltbr^tm1Kpf
• Allele Name: targeted mutation 1, Klaus Pfeffer
• Allele ID: MGI:2384140
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ltbr^tm1Kpf/Ltbr^tm1Kpf	B6.129P2-Ltbr^tm1Kpf	MGI:4453325
hm2	Ltbr^tm1Kpf/Ltbr^tm1Kpf	involves: 129P2/OlaHsd	MGI:3581397
hm3	Ltbr^tm1Kpf/Ltbr^tm1Kpf	involves: 129P2/OlaHsd * C57BL/6	MGI:3652516
cx4	Ltbr^tm1Kpf/Ltbr^tm1Kpf Tg(Lck-Tnfsf14)24Yxf/?	B6.Cg-Ltbr^tm1Kpf Tg(Lck-Tnfsf14)24Yxf	MGI:3849890

Genotype
MGI:4453325

hm1

• Allelic Composition: Ltbr^tm1Kpf/Ltbr^tm1Kpf
• Genetic Background: B6.129P2-Ltbr^tm1Kpf

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

decreased neutrophil cell number ( J:158663 )

• neutrophil numbers are greatly reduced in the lamina propria

immune system

decreased neutrophil cell number ( J:158663 )

• neutrophil numbers are greatly reduced in the lamina propria

Genotype
MGI:3581397

hm2

• Allelic Composition: Ltbr^tm1Kpf/Ltbr^tm1Kpf
• Genetic Background: involves: 129P2/OlaHsd

immune system

immune system phenotype ( J:169863 )

N • alpha-beta Th17 cell differentiation is normal

abnormal immune system morphology ( J:48837 )

increased lymphocyte cell number ( J:48837 )

• 3-5X increase in peritoneal and peripheral blood lymphocytes

increased B cell number ( J:48837 )

increased T cell number ( J:48837 )

abnormal gamma-delta T cell morphology ( J:169863 )

• fewer IL17+ thymic gamma-delta T cells in response to PMA and ionomycin stimulation

• however, the number of thymic IFN-gamma+ gamma-delta T cells

abnormal immune system organ morphology ( J:48837 )

abnormal spleen morphology ( J:48837 )

enlarged spleen ( J:48837 )

• 1.5-2X larger than normal

absent marginal zone B cells ( J:48837 )

• marginal zone B cells virtually absent

abnormal spleen B cell follicle morphology ( J:48837 )

• splenic B cells not organized in follicles

abnormal spleen periarteriolar lymphoid sheath morphology ( J:48837 )

• B cells intermixed with T cells around central arteriole

absent Peyer's patches ( J:48837 )

absent peripheral lymph nodes ( J:48837 )

• absence of peripheral lymph nodes

• sites of lymph nodes occupied by "caverenous" spaces with endothelial linings

abnormal immune system physiology ( J:48837 )

• massive infiltrations of lymphocytes into perivascular spaces of lungs, liver, pancreas, submandibular gland, mediastinum, mesentery, cortex of suprarenal gland and kidney

• infiltration mostly by CD4+ T cells and B220+ B cells

decreased IgE level ( J:86810 )

decreased interleukin-17 secretion ( J:169863 )

• in TCRbeta+CD4+CD44^hi thymocytes

hematopoietic system

increased lymphocyte cell number ( J:48837 )

• 3-5X increase in peritoneal and peripheral blood lymphocytes

increased B cell number ( J:48837 )

increased T cell number ( J:48837 )

abnormal gamma-delta T cell morphology ( J:169863 )

• fewer IL17+ thymic gamma-delta T cells in response to PMA and ionomycin stimulation

• however, the number of thymic IFN-gamma+ gamma-delta T cells

abnormal spleen morphology ( J:48837 )

enlarged spleen ( J:48837 )

• 1.5-2X larger than normal

absent marginal zone B cells ( J:48837 )

• marginal zone B cells virtually absent

abnormal spleen B cell follicle morphology ( J:48837 )

• splenic B cells not organized in follicles

abnormal spleen periarteriolar lymphoid sheath morphology ( J:48837 )

• B cells intermixed with T cells around central arteriole

decreased IgE level ( J:86810 )

growth/size/body

enlarged spleen ( J:48837 )

• 1.5-2X larger than normal

Genotype
MGI:3652516

hm3

• Allelic Composition: Ltbr^tm1Kpf/Ltbr^tm1Kpf
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

decreased spleen white pulp amount ( J:109621 )

• percentage of splenic white pulp is lower than in wild-type or Ltb mutants but higher than the two Lta mutant lines, Ltrb, or Ltb/Tnf/Lta knockouts

hematopoietic system

decreased spleen white pulp amount ( J:109621 )

• percentage of splenic white pulp is lower than in wild-type or Ltb mutants but higher than the two Lta mutant lines, Ltrb, or Ltb/Tnf/Lta knockouts

Genotype
MGI:3849890

cx4

• Allelic Composition: Ltbr^tm1Kpf/Ltbr^tm1Kpf; Tg(Lck-Tnfsf14)24Yxf/?
• Genetic Background: B6.Cg-Ltbr^tm1Kpf Tg(Lck-Tnfsf14)24Yxf

digestive/alimentary system

digestive/alimentary phenotype ( J:128976 )

N • mice show no abnormality in the intestine and no significant T cell-mediated inflammation in the gut

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:120962 )

N • the high serum cholesterol levels characteristic of the transgenic mice does not occur in these mice

immune system

immune system phenotype ( J:128976 )

N • serum IgA levels are normal and mice show no significant T cell-mediated inflammation in the gut