Phenotypes associated with this allele

• Allele Symbol: Npy4r^tm1.1Hhz
• Allele Name: targeted mutation 1.1, Herbert Herzog
• Allele ID: MGI:2183929
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz	involves: 129X1/SvJ * BALB/cJ * C57BL/6	MGI:4361942
hm2	Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz	involves: 129X1/SvJ * C57BL/6	MGI:3693952
cx3	Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz	involves: 129X1/SvJ * BALB/cJ * C57BL/6	MGI:4361940
cx4	Lep^ob/Lep^ob Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz	involves: 129X1/SvJ * C57BL/6	MGI:3694008
cx5	Lep^ob/Lep^+ Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz	involves: 129X1/SvJ * C57BL/6	MGI:3694009
cx6	Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz	involves: 129X1/SvJ * C57BL/6	MGI:4362036

Genotype
MGI:4361942

hm1

• Allelic Composition: Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz
• Genetic Background: involves: 129X1/SvJ * BALB/cJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

decreased osteoblast cell number ( J:84570 )

adipose tissue

decreased white adipose tissue amount ( J:84570 )

• in male mice

behavior/neurological

abnormal eating behavior ( J:84570 )

• in male mice

growth/size/body

decreased body weight ( J:84570 )

• in male mice

homeostasis/metabolism

increased circulating pancreatic peptide level ( J:84570 )

• plasma pancreatic peptide levels are increased compared to in wild-type mice

Genotype
MGI:3693952

hm2

• Allelic Composition: Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz
• Genetic Background: involves: 129X1/SvJ * C57BL/6

growth/size/body

decreased body weight ( J:76479 )

• 4-week old females weigh significantly less than controls

• males and females gain significantly less weight between 4 and 16 weeks of age

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:76479 )

N • levels of leptin, insulin, and glucose do not differ from wild-type

N • testosterone tends to be lower in mutants but this does not become significant

increased circulating pancreatic peptide level ( J:76479 )

♂ • males exhibit 2- to 3-fold higher plasma levels of pancreatic polypeptide (PP)

behavior/neurological

increased fluid intake ( J:115774 )

• mutants drink more water during the dark phase, but this is only significant on the second and third days of the 3 day observation period

• daily water intake is elevated only on second and third day compared to controls

• this behavior is maintained following injection of physiological saline (vehicle). beta-adrenoreceptor antagonist propanolol, or angiotensin AT1 receptor antagonist telmisartan

abnormal food intake ( J:76479 )

• males have lower food intake/24 hours than wild-type controls

increased aggression towards mice ( J:76479 )

• male mutants show very aggressive behavior, with instances of fighting between littermates causing injuries

• female mutants become aggressive toward littermates after 20-24 weeks of age

adipose tissue

decreased white adipose tissue amount ( J:76479 )

• male mice have a significantly lower mass of white adipose tissue (WAT) compared to controls

abnormal fat pad morphology ( J:76479 )

• mesenteric WAT is major contributor to reduced WAT mass (0.65 % of body weight in male mutant vs 0.84% in male wild-type and 0.53% in female mutant vs 0.76% in female wild-type)

reproductive system

abnormal mammary gland growth during pregnancy ( J:76479 )

♀ • lobuloalveolar development is accelerated compared to wild-type (lobuloalveolar content on day 16 is 18.74% area of 4th gland compared to 15.79% in wild-type)

endocrine/exocrine glands

abnormal mammary gland growth during pregnancy ( J:76479 )

♀ • lobuloalveolar development is accelerated compared to wild-type (lobuloalveolar content on day 16 is 18.74% area of 4th gland compared to 15.79% in wild-type)

integument

abnormal mammary gland growth during pregnancy ( J:76479 )

♀ • lobuloalveolar development is accelerated compared to wild-type (lobuloalveolar content on day 16 is 18.74% area of 4th gland compared to 15.79% in wild-type)

Genotype
MGI:4361940

cx3

• Allelic Composition: Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz; Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz
• Genetic Background: involves: 129X1/SvJ * BALB/cJ * C57BL/6

skeleton

abnormal osteoblast physiology ( J:84570 )

• osteoblast bone formation is increased compared to in wild-type mice

abnormal bone structure ( J:84570 )

• periosteal diameter of the cortical shaft is reduced compared to in wild-type mice

decreased compact bone thickness ( J:84570 )

• cortical bone area and thickness are decreased compared to in wild-type mice

abnormal trabecular bone morphology ( J:84570 )

• mice exhibit an increase in cancellous bone volume, trabeculae number, and trabecular thickness compared with wild-type mice and single homozygotes

increased trabecular bone thickness ( J:84570 )

abnormal bone ossification ( J:84570 )

• mice exhibit increased bone turnover with osteoblast, osteoclast, and osteoid surfaces increased compared with wild-type mice and single homozygotes

• however, osteoblast and osteoclast numbers are normal

abnormal bone mineralization ( J:84570 )

• mineral apposition rate is increased compared to in wild-type mice

homeostasis/metabolism

decreased circulating insulin level ( J:84570 )

♂ • in male mice compared with wild-type mice

decreased circulating leptin level ( J:84570 )

♂ • in male mice compared with wild-type mice and Ppyr1^tm1.1Hhz homozygotes

increased circulating pancreatic peptide level ( J:84570 )

• plasma pancreatic peptide levels are increased compared to in wild-type mice

adipose tissue

decreased brown adipose tissue amount ( J:84570 )

decreased white adipose tissue amount ( J:84570 )

♂ • male mice exhibit a decreased in white adipose tissue compared to in wild-type mice or single homozygotes

growth/size/body

decreased body weight ( J:84570 )

♂ • male mice exhibit decreased body weight compared with wild-type mice that is not as severe as in single homozygotes

behavior/neurological

abnormal eating behavior ( J:84570 )

♂ • in male mice compared to in wild-type mice and single homozygotes

cellular

abnormal osteoblast physiology ( J:84570 )

• osteoblast bone formation is increased compared to in wild-type mice

Genotype
MGI:3694008

cx4

• Allelic Composition: Lep^ob/Lep^ob; Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz
• Genetic Background: involves: 129X1/SvJ * C57BL/6

growth/size/body

increased susceptibility to weight gain ( J:76479 )

• male mutants gain significantly more weight than Lep^ob homozygous controls between 4 and 16 weeks

endocrine/exocrine glands

abnormal branching of the mammary ductal tree ( J:76479 )

♀ • in nulliparous females, no ductal development occurs, and gland remains in prepubescent state

♀ • females having had one full-term pregnancy show varying degrees of mammary development; some females show minimal ductal elongation, while others show large areas of lobules with presence of milk

♀ • females are incapable of ensuring survival of offspring due to defects in mammary gland structure and physiology

increased seminal vesicle weight ( J:76479 )

♂ • gland is significantly increased in weight compared to Lep^ob homozygotes, and is comparable to wild-type

abnormal ovarian follicle morphology ( J:76479 )

♀ • large empty follicles characteristic of Lep^ob mice are present

increased Leydig cell number ( J:76479 )

♂ • Leydig cell density is increased over Lep^ob homozygotes

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:76479 )

N • insulin and leptin levels are not increased over Lep^ob homozygotes

abnormal circulating testosterone level ( J:76479 )

• levels are significantly increased over levels found in Lep^ob homozygotes; levels are similar to wild-type

increased circulating corticosterone level ( J:76479 )

• hypercorticosteronemia is significantly attenuated compared to Lep^ob homozygotes

adipose tissue

abnormal adipose tissue amount ( J:76479 )

• WAT amount is significantly higher than in control Lep^ob heterozygotes

reproductive system

increased seminal vesicle weight ( J:76479 )

♂ • gland is significantly increased in weight compared to Lep^ob homozygotes, and is comparable to wild-type

abnormal ovarian follicle morphology ( J:76479 )

♀ • large empty follicles characteristic of Lep^ob mice are present

increased Leydig cell number ( J:76479 )

♂ • Leydig cell density is increased over Lep^ob homozygotes

abnormal vagina orifice morphology ( J:76479 )

♀ • in infertile females, vaginal orifice is small with cytology similar to Lep^ob homozygotes

increased sperm number ( J:76479 )

♂ • sperm density is increased over Lep^ob homozygotes

abnormal estrous cycle ( J:76479 )

♀

enhanced fertility ( J:76479 )

• all males tested produced offspring, while only 4/8 females produced live offspring compared to severely reduced fertility in Lep^ob homozygous male and female mice

integument

abnormal branching of the mammary ductal tree ( J:76479 )

♀ • in nulliparous females, no ductal development occurs, and gland remains in prepubescent state

♀ • females having had one full-term pregnancy show varying degrees of mammary development; some females show minimal ductal elongation, while others show large areas of lobules with presence of milk

♀ • females are incapable of ensuring survival of offspring due to defects in mammary gland structure and physiology

Genotype
MGI:3694009

cx5

• Allelic Composition: Lep^ob/Lep⁺; Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz
• Genetic Background: involves: 129X1/SvJ * C57BL/6

growth/size/body

increased body weight ( J:76479 )

• males have a tendency toward increased body weight compared to controls

adipose tissue

increased white adipose tissue amount ( J:76479 )

• WAT mass is increased compared to control Lep^ob heterozygotes

Genotype
MGI:4362036

cx6

• Allelic Composition: Npy2r^tm1.1Hhz/Npy2r^tm1.1Hhz; Npy4r^tm1.1Hhz/Npy4r^tm1.1Hhz
• Genetic Background: involves: 129X1/SvJ * C57BL/6

homeostasis/metabolism

increased circulating insulin-like growth factor I level ( J:129609 )

• after fasting compared with similarly treated wild-type mice

increased growth hormone level ( J:129609 )

• pituitary gland cell growth hormone levels are increased in fasting mice compared to non-fasting mice and unlike in similarly treated wild-type mice

• pituitary gland cell growth hormone levels are increased in nonfasting mice compared with wild-type mice

abnormal testosterone level ( J:129609 )

• testosterone levels in Leydig cells are increased in fasting mice compared to in similarly treated wild-type mice