Phenotypes associated with this allele

• Allele Symbol: Mesp1^tm3Ysa
• Allele Name: targeted mutation 3, Yumiko Saga
• Allele ID: MGI:2182080
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mesp1^tm3Ysa/Mesp1^tm3Ysa	involves: C57BL/6 * CBA	MGI:2183222
ht2	Mesp1^tm1Ysa/Mesp1^tm3Ysa	involves: C57BL/6 * CBA * ICR	MGI:3702403
cx3	Mesp1^tm3Ysa/Mesp1^tm3Ysa Mesp2^tm3(cre)Ysa/Mesp2^tm3(cre)Ysa	involves: C57BL/6 * CBA	MGI:3702332

Genotype
MGI:2183222

hm1

• Allelic Composition: Mesp1^tm3Ysa/Mesp1^tm3Ysa
• Genetic Background: involves: C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:49903 , J:55840 )

• all homozygous mutant embryos die around E10.5 (J:49903)

embryo

abnormal cardiogenic mesoderm morphology ( J:55840 )

• development of cardiac mesoderm is strongly affected

delayed embryo turning ( J:49903 )

• in homozygotes, embryonic turning is often delayed

failure of initiation of embryo turning ( J:49903 )

• embryonic turning is entirely absent in some embryos

abnormal mesoderm development ( J:55840 )

• development of the cardiac mesoderm and paraxial mesoderm are strongly affected

• in contrast, the axial mesoderm is generated normally

embryonic growth arrest ( J:49903 , J:55840 )

• homozygotes display an embryonic growth arrest at E8.5-E9.0 (J:49903)

embryonic growth retardation ( J:49903 , J:55840 )

• homozygotes exhibit growth retardation after E7.5 (J:49903)

• embryos stop growing after reaching the size corresponding to the wild-type E8.5-9 embryo (J:55840)

decreased embryo size ( J:49903 )

• at E7.5 and E9.5, homozygotes are smaller than wild-type or heterozygous embryos

abnormal paraxial mesoderm morphology ( J:55840 )

• development of paraxial mesoderm is strongly affected

abnormal neural fold formation ( J:49903 )

• at E7.5, homozygotes display underdeveloped, smaller head folds

abnormal neural tube morphology ( J:49903 )

• at E9.5, most homozygotes exhibit curved neural tubes esp. in the cervical and thoracic region

delayed neural tube closure ( J:49903 )

• in homozygotes, neural fold closure is either delayed or entirely absent in some embryos

abnormal primitive streak morphology ( J:55840 )

• exhibit an abnormal accumulation of cells in the primitive streak

decreased somite size ( J:49903 )

• at E9.5, homozygotes display a significant reduction in somite width along the A-P axis

growth/size/body

embryonic growth retardation ( J:49903 , J:55840 )

• homozygotes exhibit growth retardation after E7.5 (J:49903)

• embryos stop growing after reaching the size corresponding to the wild-type E8.5-9 embryo (J:55840)

decreased embryo size ( J:49903 )

• at E7.5 and E9.5, homozygotes are smaller than wild-type or heterozygous embryos

microcephaly ( J:49903 )

cardiovascular system

abnormal cardiogenic mesoderm morphology ( J:55840 )

• development of cardiac mesoderm is strongly affected

abnormal heart development ( J:49903 , J:55840 )

abnormal heart looping ( J:49903 , J:55840 )

• at E9.5, homozygotes display abnormal heart looping relative to size-matched E9.0 wild-type embryos (J:49903)

• the separated heart tubes have randomized looping and embryos with a single heart tube also display several variations of looping (J:55840)

abnormal heart tube morphology ( J:55840 )

• embryos show various anomalies of heart tube formation, with the heart tubes ranging from two completely separated tubes to single tube

cardia bifida ( J:49903 , J:55840 )

• at E9.5, many mutant embryos exhibit two abnormally symmetrical heart tubes with uncoordinated beat periodicity (J:49903)

• separated tubes show various degrees of cardia bifidia, from full to partial bifurcations (J:55840)

nervous system

abnormal neural tube morphology ( J:49903 )

• at E9.5, most homozygotes exhibit curved neural tubes esp. in the cervical and thoracic region

delayed neural tube closure ( J:49903 )

• in homozygotes, neural fold closure is either delayed or entirely absent in some embryos

Genotype
MGI:3702403

ht2

• Allelic Composition: Mesp1^tm1Ysa/Mesp1^tm3Ysa
• Genetic Background: involves: C57BL/6 * CBA * ICR

embryo

abnormal mesoderm development ( J:55840 )

• mesodermal cells fail to migrate out of the primitive streak

abnormal primitive streak morphology ( J:55840 )

• exhibit an abnormal accumulation of beta-gal-positive mesodermal cells in the primitive streak as these cells do not actively migrate out from the primitive streak

Genotype
MGI:3702332

cx3

• Allelic Composition: Mesp1^tm3Ysa/Mesp1^tm3Ysa; Mesp2^tm3(cre)Ysa/Mesp2^tm3(cre)Ysa
• Genetic Background: involves: C57BL/6 * CBA

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:62984 )

• embryos exhibit a similar morphology and marker gene expression to Mesp1/Mesp2^tm1Ysa homozygous embryos, however no data is presented in J:62984

growth/size/body

abnormal embryonic growth/weight/body size ( J:62984 )

embryo

abnormal embryo development ( J:62984 )

cardiovascular system

abnormal heart morphology ( J:62984 )