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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Zic3tm1Bca
targeted mutation 1, Brett Casey
MGI:2180720
Summary 8 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Zic3tm1Bca/Zic3tm1Bca involves: 129S7/SvEvBrd MGI:2180833
hm2
 		Zic3tm1Bca/Zic3tm1Bca involves: 129S7/SvEvBrd * C57BL/6 MGI:3618629
hm3
 		Zic3tm1Bca/Zic3tm1Bca involves: 129S7/SvEvBrd * C57BL/6J MGI:3618633
ht4
 		Zic3tm1Bca/Zic3+ involves: 129S7/SvEvBrd * C57BL/6 MGI:3618631
ot5
 		Zic3tm1Bca/Y involves: 129S7/SvEvBrd MGI:2180831
ot6
 		Zic3tm1Bca/Y involves: 129S7/SvEvBrd * C57BL/6 MGI:3618630
ot7
 		Zic3tm1Bca/Y involves: 129S7/SvEvBrd * C57BL/6J MGI:3618634
ot8
 		Zic3tm1Bca/Y involves: 129S7/SvEvBrd * Swiss MGI:3618632


Genotype
MGI:2180833
hm1
Allelic
Composition		 Zic3tm1Bca/Zic3tm1Bca
Genetic
Background		 involves: 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zic3tm1Bca mutation (0 available); any Zic3 mutation (37 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
preweaning lethality, incomplete penetrance ( J:192577 )
• despite normal numbers at E9.5, fewer than expected mice at E15.5 and weaning

cardiovascular system
abnormal inferior vena cava morphology ( J:192577 )
• interruption with separate entrance of hepatic veins in some mice
abnormal heart looping ( J:192577 )
• in 4 of 18 mice at E9.5
abnormal direction of heart looping ( J:192577 )
• sinisteral looping in some mice
atrial septal defect ( J:192577 )
• in some mice
abnormal heart position or orientation ( J:192577 )
• atrium isomerism in some mice
dextrocardia ( J:192577 )
• in some mice

digestive/alimentary system
cleft palate ( J:192577 )
• in 5 of 58 mice at E15.5
abnormal stomach position or orientation ( J:192577 )
• midline and right-sided stomach in some mice
right-sided stomach ( J:192577 )
• in some mice

embryo
embryonic growth retardation ( J:192577 )
• in 12 of 18 mice
abnormal neural tube morphology ( J:192577 )
• in 5 of 58 mice at E15.5
open neural tube ( J:192577 )
• in some mice at E9.5

growth/size/body
embryonic growth retardation ( J:192577 )
• in 12 of 18 mice
abnormal head morphology ( J:192577 )
• in some mice at E9.5
cleft palate ( J:192577 )
• in 5 of 58 mice at E15.5
heterotaxia ( J:192577 )
• laterality defects in 3 of 15 mice
right-sided stomach ( J:192577 )
• in some mice
situs inversus ( J:192577 )
• in some mice

craniofacial
cleft palate ( J:192577 )
• in 5 of 58 mice at E15.5

nervous system
abnormal neural tube morphology ( J:192577 )
• in 5 of 58 mice at E15.5
open neural tube ( J:192577 )
• in some mice at E9.5
exencephaly ( J:192577 )
• in 5 of 58 mice at E15.5

respiratory system
abnormal lung position or orientation ( J:192577 )
• pulmonary isomerism in some mice




Genotype
MGI:3618629
hm2
Allelic
Composition		 Zic3tm1Bca/Zic3tm1Bca
Genetic
Background		 involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zic3tm1Bca mutation (0 available); any Zic3 mutation (37 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
postnatal lethality, incomplete penetrance ( J:75951 )
• about 30% of males are lost between birth and weaning
prenatal lethality, incomplete penetrance ( J:75951 )
• significant lethality seen by E10.5 with continued loss of mice through birth
• this loss is somewhat less severe than in hemizygous males

embryo
abnormal embryo turning ( J:75951 )
• failure, delay, or abnormal direction of turning are seen
• some embryos that survive beyond the turning stage show rotation defects including abnormal thoracic and lumbar flexion, and/or abnormal looping of the distal tail (including the hindlimb buds)
abnormal left-right axis patterning ( J:75951 )
• sidedness of the malformations appears to be random
embryonic growth retardation ( J:75951 )
• some embryos with no other gross abnormalities show evidence of growth delay
delayed neural tube closure ( J:75951 )
• seen in some embryos at E8.5
open neural tube ( J:75951 )
• at E9.5, failure of fusion is seen in some embryos in the midbrain-hindbrain region

nervous system
delayed neural tube closure ( J:75951 )
• seen in some embryos at E8.5
open neural tube ( J:75951 )
• at E9.5, failure of fusion is seen in some embryos in the midbrain-hindbrain region
abnormal midbrain morphology ( J:75951 )
• abnormal midbrain size and structure in some embryos
abnormal forebrain morphology ( J:75951 )
• abnormal forebrain size and structure in some embryos
abnormal basal ganglion morphology ( J:75951 )
• exencephalic mice have a disorganized basal ganglia
abnormal diencephalon morphology ( J:75951 )
• exencephalic mice have an anteriorly displaced diencephalon
disorganized thalamus ( J:75951 )
• exencephalic mice have a disorganized thalamus
abnormal hippocampus morphology ( J:75951 )
• exencephalic mice have a disorganized hippocampus
abnormal cerebral cortex morphology ( J:75951 )
• exencephalic mice have a disorganized cerebral cortex
absent cerebellum ( J:75951 )
• seen in exencephalic mice
anencephaly ( J:75951 )
• seen in a few stillborn pups
exencephaly ( J:75951 )
• seen in some pups delivered by Ceasarian sections
abnormal olfactory nerve morphology ( J:75951 )
• xencephalic mice have rudimentary olfactory nerves

cardiovascular system
abnormal cardiovascular system morphology ( J:75951 )
• a variety of cardiac defects are seen in a subset of embryos and these embryos always also have other abnormalities
interrupted aortic arch ( J:75951 )
• right aortic arch and interrupted aortic arch are seen in some embryos
right aortic arch ( J:75951 )
• right aortic arch and interrupted aortic arch are seen in some embryos
abnormal heart development ( J:75951 )
• abnormal systemic venous connections
d-loop transposition of the great arteries ( J:75951 )
• one of the most common heart defects is dextro-transposition of the great arteries
abnormal heart position or orientation ( J:75951 )
• at E10.5 in several embryos the heart is positioned to the left side of the thorax, however in some abnormally turned embryos the heart is properly positioned at the midline and in 2 cases the heart is positioned to the right side of the thorax

respiratory system
lung situs inversus ( J:75951 )
• in a few embryos complete lung reversal is seen

digestive/alimentary system
right-sided stomach ( J:75951 )
• in some mice right sided stomach is seen

liver/biliary system
abnormal liver morphology ( J:75951 )
• in some mice reversed liver lobation is seen

immune system
spleen hypoplasia ( J:75951 )
• the spleen is always present but often hypoplastic

skeleton
abnormal axial skeleton morphology ( J:75951 )
• 10 null mice (includes males and females) had mostly unilateral axial skeletal defects
abnormal rib morphology ( J:75951 )
• partial rib duplications and inappropriately posterior sites of rib insertion are found
abnormal vertebrae morphology ( J:75951 )
• partial duplications and partial fusions are seen
vertebral transformation ( J:75951 )
• apparent homeotic transformations

growth/size/body
right-sided stomach ( J:75951 )
• in some mice right sided stomach is seen
embryonic growth retardation ( J:75951 )
• some embryos with no other gross abnormalities show evidence of growth delay
left pulmonary isomerism ( J:75951 )
lung situs inversus ( J:75951 )
• in a few embryos complete lung reversal is seen
right pulmonary isomerism ( J:75951 )

hematopoietic system
spleen hypoplasia ( J:75951 )
• the spleen is always present but often hypoplastic




Genotype
MGI:3618633
hm3
Allelic
Composition		 Zic3tm1Bca/Zic3tm1Bca
Genetic
Background		 involves: 129S7/SvEvBrd * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zic3tm1Bca mutation (0 available); any Zic3 mutation (37 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
embryonic lethality, complete penetrance ( J:106144 )
• by E8.5 to E9.5 many of the most severely affected embryos (Type I) are beginning to be resorbed

embryo
abnormal embryo development ( J:106144 )
• four types of mutant embryos are seen; Type I are the most severely affected (21.5% of hemizygous and homozygous mice), Type II are less severely affected (40.5%), Type III do not show defects until E8.0-E9.5 (16.5%), and Type IV do not show any gross defects at E6.5 through E9.5
abnormal gastrulation ( J:106144 )
• in Type I embryos abnormal expression of markers of the primitive streak suggest either failure to gastrulate or initiation of gastrulation that fails to progress normally
abnormal mesoderm development ( J:106144 )
• in Type II embryos excess mesoderm formation is seen at the primitive streak, mesoderm is seen outside the primitive streak, and mesoderm is also found in the anterior of the embryo disrupting the connection of the anterior visceral endoderm with the underlying ectoderm
abnormal left-right axis patterning ( J:106144 )
• in some Type II embryos no evidence of node-like tissue is seen at the midline, cellular accumulation is different on the left and right sides of the embryo, and the primitive streak is not in the midline
abnormal rostral-caudal axis patterning ( J:106144 )
• by E8.0-E9.5, Type III embryos show signs of caudal truncation and/or abnormal somitogenesis
caudal body truncation ( J:106144 )
• Type III embryos all have a shortened posterior axis with variable degrees of caudal truncation from anterior head structures only to presence of the head and heart
rostral-caudal axis duplication ( J:106144 )
• some mildly affected Type II embryos show signs of axial duplication
embryonic growth arrest ( J:106144 )
• some more severely affected Type II embryos stop developing around E8.5
embryonic growth retardation ( J:106144 )
• at E7.5-E7.5 the Type I embryos show significant developmental delay and remain at the epiblast or early streak stage
abnormal embryonic epiblast morphology ( J:106144 )
• in Type I embryos the epiblasts show signs of clefts and/or indistinct extraembryonic and embryonic ectoderm layers
abnormal neural plate morphology ( J:106144 )
• some mildly affected Type II embryos show asymmetric development of the posterior neural plate and the underlying mesoderm
• Type III embryos fail to develop a normal posterior neural plate
abnormal embryonic neuroepithelial layer differentiation ( J:106144 )
• poor differentiation of the neuroectoderm is seen in areas with increased cell number in the primitive streak
split notochord ( J:106144 )
• variable degrees of axis duplication from partial formation of a second notochord through conjoined twinning occur
• isolated anterior and isolated posterior duplications are also seen and in some cases notochord tissue is absent from the trunk region
abnormal primitive streak morphology ( J:106144 )
• in Type I embryos expression of markers for the primitive streak suggest that the anterior primitive streak and node are absent
• some less severely affected Type II embryos display an abnormal primitive streak with excess mesoderm formation, failure to properly elongate the primitive streak, and failure of the normal regionalization process
• Type III embryos do not display abnormalities until E8.0-E9.5 when an abnormal, rudimentary primitive streak region is seen
• at E9.0 in mildly affected embryos the primitive steak is thicker than in wild-type embryos, this increase in cell number may be unilateral or bilateral
abnormal anterior primitive streak morphology ( J:106144 )
• in Type I embryos expression of markers for the primitive streak suggest that the anterior primitive streak and node are absent
absent primitive node ( J:106144 )
• in Type I embryos expression of markers for the primitive streak suggest that the anterior primitive streak and node are absent
abnormal somite development ( J:106144 )
• by E8.0-E9.5, Type III embryos show signs of caudal truncation and/or abnormal somitogenesis
• Type III embryos that do develop somites have reduced somite numbers and size
abnormal left-right axis symmetry of the somites ( J:106144 )
• asymmetries, most obviously seen in the somites, occur in about 10% of embryos probably as a result of partial axis duplications
abnormal primitive streak elongation ( J:106144 )
• some less severely affected Type II embryos display a failure to properly elongate the primitive streak
abnormal extraembryonic tissue morphology ( J:106144 )
• in embryos that have an intermediate phenotype (Type II) at mid streak and late streak a cells protrude into the amniotic cavity
small allantois ( J:106144 )
• in Type III embryos the allantois is frequently small but the extraembryonic tissues are otherwise normal
abnormal anterior visceral endoderm morphology ( J:106144 )
• all markers of the anterior visceral endoderm are abnormal

nervous system
abnormal neural plate morphology ( J:106144 )
• some mildly affected Type II embryos show asymmetric development of the posterior neural plate and the underlying mesoderm
• Type III embryos fail to develop a normal posterior neural plate
abnormal embryonic neuroepithelial layer differentiation ( J:106144 )
• poor differentiation of the neuroectoderm is seen in areas with increased cell number in the primitive streak

cardiovascular system
abnormal heart looping ( J:106144 )
• embryos with asymmetries often have abnormal cardiac looping

growth/size/body
embryonic growth retardation ( J:106144 )
• at E7.5-E7.5 the Type I embryos show significant developmental delay and remain at the epiblast or early streak stage




Genotype
MGI:3618631
ht4
Allelic
Composition		 Zic3tm1Bca/Zic3+
Genetic
Background		 involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zic3tm1Bca mutation (0 available); any Zic3 mutation (37 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
postnatal lethality, incomplete penetrance ( J:75951 )
• about 30% of males are lost between birth and weaning

embryo
abnormal embryo turning ( J:75951 )
• failure, delay, or abnormal direction of turning are seen
• some embryos that survive beyond the turning stage show rotation defects including abnormal thoracic and lumbar flexion, and/or abnormal looping of the distal tail (including the hindlimb buds)
abnormal left-right axis patterning ( J:75951 )
• sidedness of the malformations appears to be random
embryonic growth retardation ( J:75951 )
• some embryos with no other gross abnormalities show evidence of growth delay
delayed neural tube closure ( J:75951 )
• seen in some embryos at E8.5
open neural tube ( J:75951 )
• at E9.5, failure of fusion is seen in some embryos in the midbrain-hindbrain region

nervous system
delayed neural tube closure ( J:75951 )
• seen in some embryos at E8.5
open neural tube ( J:75951 )
• at E9.5, failure of fusion is seen in some embryos in the midbrain-hindbrain region
abnormal midbrain morphology ( J:75951 )
• abnormal midbrain size and structure in some embryos
abnormal forebrain morphology ( J:75951 )
• abnormal forebrain size and structure in some embryos
abnormal basal ganglion morphology ( J:75951 )
• exencephalic mice have a disorganized basal ganglia
abnormal diencephalon morphology ( J:75951 )
• exencephalic mice have an anteriorly displaced diencephalon
disorganized thalamus ( J:75951 )
• exencephalic mice have a disorganized thalamus
abnormal hippocampus morphology ( J:75951 )
• exencephalic mice have a disorganized hippocampus
abnormal cerebral cortex morphology ( J:75951 )
• exencephalic mice have a disorganized cerebral cortex
absent cerebellum ( J:75951 )
• seen in exencephalic mice
anencephaly ( J:75951 )
• seen in a few stillborn pups
exencephaly ( J:75951 )
• seen in some pups delivered by Ceasarian sections
abnormal olfactory nerve morphology ( J:75951 )
• xencephalic mice have rudimentary olfactory nerves

cardiovascular system
abnormal cardiovascular system morphology ( J:75951 )
• a variety of cardiac defects are seen in a subset of embryos and these embryos always also have other abnormalities
interrupted aortic arch ( J:75951 )
• an interrupted aortic arch is seen in some embryos
right aortic arch ( J:75951 )
• a right aortic arch is seen in some embryos
abnormal heart development ( J:75951 )
• abnormal systemic venous connections
d-loop transposition of the great arteries ( J:75951 )
• one of the most common heart defects is dextro-transposition of the great arteries
abnormal heart position or orientation ( J:75951 )
• at E10.5 in several embryos the heart is positioned to the left side of the thorax, however in some abnormally turned embryos the heart is properly positioned at the midline and in 2 cases the heart is positioned to the right side of the thorax

respiratory system
lung situs inversus ( J:75951 )
• in a few embryos complete lung reversal is seen

digestive/alimentary system
right-sided stomach ( J:75951 )
• in some mice right sided stomach is seen

liver/biliary system
abnormal liver morphology ( J:75951 )
• in some mice reversed liver lobation is seen

immune system
spleen hypoplasia ( J:75951 )
• the spleen is always present but often hypoplastic

skeleton
abnormal axial skeleton morphology ( J:75951 )
• 2 heterozygous mice had mostly unilateral axial skeletal defects
abnormal rib morphology ( J:75951 )
• partial rib duplications and inappropriately posterior sites of rib insertion are found
abnormal vertebrae morphology ( J:75951 )
• partial duplications and partial fusions are seen
vertebral transformation ( J:75951 )
• apparent homeotic transformations

limbs/digits/tail
kinked tail ( J:75951 )
• about 40% of heterozygous females have kinked tails

growth/size/body
right-sided stomach ( J:75951 )
• in some mice right sided stomach is seen
embryonic growth retardation ( J:75951 )
• some embryos with no other gross abnormalities show evidence of growth delay
left pulmonary isomerism ( J:75951 )
lung situs inversus ( J:75951 )
• in a few embryos complete lung reversal is seen
right pulmonary isomerism ( J:75951 )

hematopoietic system
spleen hypoplasia ( J:75951 )
• the spleen is always present but often hypoplastic




Genotype
MGI:2180831
ot5
Allelic
Composition		 Zic3tm1Bca/Y
Genetic
Background		 involves: 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zic3tm1Bca mutation (0 available); any Zic3 mutation (37 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
prenatal lethality, complete penetrance ( J:75951 )
• Background Sensitivity: no live born hemizygotes, unlike in crosses to C57BL/6 or outbred Swiss mice
preweaning lethality, incomplete penetrance ( J:192577 )
• despite normal numbers at E9.5, fewer than expected mice at E15.5 and weaning

cardiovascular system
right aortic arch ( J:192577 )
• in some mice
abnormal inferior vena cava morphology ( J:192577 )
• interruption in some mice
abnormal heart looping ( J:192577 )
• in 4 of 18 mice at E9.5
abnormal direction of heart looping ( J:192577 )
• sinisteral looping in some mice
dextrocardia ( J:192577 )
• in some mice
ventricular septal defect ( J:192577 )
• in some mice

craniofacial
cleft palate ( J:192577 )
• in 5 of 58 mice at E15.5

digestive/alimentary system
cleft palate ( J:192577 )
• in 5 of 58 mice at E15.5
abnormal stomach position or orientation ( J:192577 )
• midline and right-sided stomach in some mice
right-sided stomach ( J:192577 )
• in some mice

embryo
abnormal endoderm development ( J:194989 )
• cells appear larger
embryonic growth retardation ( J:192577 )
• in 12 of 18 mice
abnormal neural tube morphology ( J:192577 )
• in 5 of 58 mice at E15.5
abnormal primitive node morphology ( J:194989 )
• the teardrop structure at the distal tip of the embryo is irregular and forms non-cohesive groups of node cells

growth/size/body
embryonic growth retardation ( J:192577 )
• in 12 of 18 mice
abnormal head morphology ( J:192577 )
• in some mice at E9.5
cleft palate ( J:192577 )
• in 5 of 58 mice at E15.5
heterotaxia ( J:192577 )
• laterality defects in 3 of 15 mice
right-sided stomach ( J:192577 )
• in some mice
lung situs inversus ( J:192577 )
• in some mice
situs inversus ( J:192577 )
• in some mice

nervous system
abnormal neural tube morphology ( J:192577 )
• in 5 of 58 mice at E15.5
exencephaly ( J:192577 )
• in 5 of 58 mice at E15.5

respiratory system
lung situs inversus ( J:192577 )
• in some mice

cellular




Genotype
MGI:3618630
ot6
Allelic
Composition		 Zic3tm1Bca/Y
Genetic
Background		 involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zic3tm1Bca mutation (0 available); any Zic3 mutation (37 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
postnatal lethality, incomplete penetrance ( J:75951 )
• about 30% of males are lost between birth and weaning
prenatal lethality, incomplete penetrance ( J:75951 )
• significant lethality seen by E10.5 with continued loss of mice through birth
• this loss is somewhat more severe than in homozygous females
• Background Sensitivity: lethality is more severe in crosses to C57BL/6 compared to outbred Swiss (viability of 0.26 versus 0.46, respectively)

embryo
abnormal embryo turning ( J:75951 )
• failure, delay, or abnormal direction of turning are seen
• some embryos that survive beyond the turning stage show rotation defects including abnormal thoracic and lumbar flexion, and/or abnormal looping of the distal tail (including the hindlimb buds)
abnormal left-right axis patterning ( J:75951 )
• sidedness of the malformations appears to be random
embryonic growth retardation ( J:75951 )
• some embryos with no other gross abnormalities show evidence of growth delay
delayed neural tube closure ( J:75951 )
• seen in some embryos at E8.5
open neural tube ( J:75951 )
• at E9.5, failure of fusion is seen in some embryos in the midbrain-hindbrain region

nervous system
delayed neural tube closure ( J:75951 )
• seen in some embryos at E8.5
open neural tube ( J:75951 )
• at E9.5, failure of fusion is seen in some embryos in the midbrain-hindbrain region
abnormal midbrain morphology ( J:75951 )
• abnormal midbrain size and structure in some embryos
abnormal forebrain morphology ( J:75951 )
• abnormal forebrain size and structure in some embryos
abnormal basal ganglion morphology ( J:75951 )
• exencephalic mice have a disorganized basal ganglia
abnormal diencephalon morphology ( J:75951 )
• exencephalic mice have an anteriorly displaced diencephalon
disorganized thalamus ( J:75951 )
• exencephalic mice have a disorganized thalamus
abnormal hippocampus morphology ( J:75951 )
• exencephalic mice have a disorganized hippocampus
abnormal cerebral cortex morphology ( J:75951 )
• exencephalic mice have a disorganized cerebral cortex
absent cerebellum ( J:75951 )
• seen in exencephalic mice
anencephaly ( J:75951 )
• seen in a few stillborn pups
exencephaly ( J:75951 )
• seen in some pups delivered by Ceasarian sections
abnormal olfactory nerve morphology ( J:75951 )
• xencephalic mice have rudimentary olfactory nerves

cardiovascular system
abnormal cardiovascular system morphology ( J:75951 )
• a variety of cardiac defects are seen in a subset of embryos and these embryos always also have other abnormalities
interrupted aortic arch ( J:75951 )
• seen in some embryos
right aortic arch ( J:75951 )
• seen in some embryos
abnormal heart development ( J:75951 )
• abnormal systemic venous connections
d-loop transposition of the great arteries ( J:75951 )
• one of the most common heart defects is dextro-transposition of the great arteries
abnormal heart position or orientation ( J:75951 )
• at E10.5 in several embryos the heart is positioned to the left side of the thorax, however in some abnormally turned embryos the heart is properly positioned at the midline and in 2 cases the heart is positioned to the right side of the thorax

respiratory system
lung situs inversus ( J:75951 )
• in a few embryos complete lung reversal is seen

digestive/alimentary system
right-sided stomach ( J:75951 )
• in some mice right sided stomach is seen

liver/biliary system
abnormal liver morphology ( J:75951 )
• in some mice reversed liver lobation is seen

immune system
spleen hypoplasia ( J:75951 )
• the spleen is always present but often hypoplastic

skeleton
abnormal axial skeleton morphology ( J:75951 )
• 10 null mice (includes males and females) had mostly unilateral axial skeletal defects
abnormal rib morphology ( J:75951 )
• partial rib duplications and inappropriately posterior sites of rib insertion are found
abnormal vertebrae morphology ( J:75951 )
• partial duplications and partial fusions are seen
vertebral transformation ( J:75951 )
• apparent homeotic transformations

limbs/digits/tail
kinked tail ( J:75951 )
• all viable males have kinked tails

growth/size/body
right-sided stomach ( J:75951 )
• in some mice right sided stomach is seen
embryonic growth retardation ( J:75951 )
• some embryos with no other gross abnormalities show evidence of growth delay
left pulmonary isomerism ( J:75951 )
lung situs inversus ( J:75951 )
• in a few embryos complete lung reversal is seen
right pulmonary isomerism ( J:75951 )

hematopoietic system
spleen hypoplasia ( J:75951 )
• the spleen is always present but often hypoplastic




Genotype
MGI:3618634
ot7
Allelic
Composition		 Zic3tm1Bca/Y
Genetic
Background		 involves: 129S7/SvEvBrd * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zic3tm1Bca mutation (0 available); any Zic3 mutation (37 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
embryonic lethality, complete penetrance ( J:106144 )
• by E8.5 to E9.5 many of the most severely affected embryos (Type I) are beginning to be resorbed

embryo
abnormal embryo development ( J:106144 )
• four types of mutant embryos are seen; Type I are the most severely affected (21.5% of hemizygous and homozygous mice), Type II are less severely affected (40.5%), Type III do not show defects until E8.0-E9.5 (16.5%), and Type IV do not show any gross defects at E6.5 through E9.5
abnormal gastrulation ( J:106144 )
• in Type I embryos abnormal expression of markers of the primitive streak suggest either failure to gastrulate or initiation of gastrulation that fails to progress normally
abnormal mesoderm development ( J:106144 )
• in Type II embryos excess mesoderm formation is seen at the primitive streak, mesoderm is seen outside the primitive streak, and mesoderm is also found in the anterior of the embryo disrupting the connection of the anterior visceral endoderm with the underlying ectoderm
abnormal left-right axis patterning ( J:106144 )
• in some Type II embryos no evidence of node-like tissue is seen at the midline, cellular accumulation is different on the left and right sides of the embryo, and the primitive streak is not in the midline
abnormal rostral-caudal axis patterning ( J:106144 )
• by E8.0-E9.5, Type III embryos show signs of caudal truncation and/or abnormal somitogenesis
caudal body truncation ( J:106144 )
• Type III embryos all have a shortened posterior axis with variable degrees of caudal truncation from anterior head structures only to presence of the head and heart
rostral-caudal axis duplication ( J:106144 )
• some mildly affected Type II embryos show signs of axial duplication
embryonic growth arrest ( J:106144 )
• some more severely affected Type II embryos stop developing around E8.5
embryonic growth retardation ( J:106144 )
• at E7.5-E7.5 the Type I embryos show significant developmental delay and remain at the epiblast or early streak stage
abnormal embryonic epiblast morphology ( J:106144 )
• in Type I embryos the epiblasts show signs of clefts and/or indistinct extraembryonic and embryonic ectoderm layers
abnormal neural plate morphology ( J:106144 )
• some mildly affected Type II embryos show asymmetric development of the posterior neural plate and the underlying mesoderm
• Type III embryos fail to develop a normal posterior neural plate
abnormal embryonic neuroepithelial layer differentiation ( J:106144 )
• poor differentiation of the neuroectoderm is seen in areas with increased cell number in the primitive streak
split notochord ( J:106144 )
• variable degrees of axis duplication from partial formation of a second notochord through conjoined twinning occur
• isolated anterior and isolated posterior duplications are also seen and in some cases notochord tissue is absent from the trunk region
abnormal primitive streak morphology ( J:106144 )
• in Type I embryos expression of markers for the primitive streak suggest that the anterior primitive streak and node are absent
• some less severely affected Type II embryos display an abnormal primitive streak with excess mesoderm formation, failure to properly elongate the primitive streak, and failure of the normal regionalization process
• Type III embryos do not display abnormalities until E8.0-E9.5 when an abnormal, rudimentary primitive streak region is seen
• at E9.0 in mildly affected embryos the primitive steak is thicker than in wild-type embryos, this increase in cell number may be unilateral or bilateral
abnormal anterior primitive streak morphology ( J:106144 )
• in Type I embryos expression of markers for the primitive streak suggest that the anterior primitive streak and node are absent
absent primitive node ( J:106144 )
• in Type I embryos expression of markers for the primitive streak suggest that the anterior primitive streak and node are absent
abnormal somite development ( J:106144 )
• by E8.0-E9.5, Type III embryos show signs of caudal truncation and/or abnormal somitogenesis
• Type III embryos that do develop somites have reduced somite numbers and size
abnormal left-right axis symmetry of the somites ( J:106144 )
• asymmetries, most obviously seen in the somites, occur in about 10% of embryos probably as a result of partial axis duplications
abnormal primitive streak elongation ( J:106144 )
• some less severely affected Type II embryos display a failure to properly elongate the primitive streak
abnormal extraembryonic tissue morphology ( J:106144 )
• in embryos that have an intermediate phenotype (Type II) at mid streak and late streak a cells protrude into the amniotic cavity
small allantois ( J:106144 )
• in Type III embryos the allantois is frequently small but the extraembryonic tissues are otherwise normal
abnormal anterior visceral endoderm morphology ( J:106144 )
• all markers of the anterior visceral endoderm are abnormal

nervous system
abnormal neural plate morphology ( J:106144 )
• some mildly affected Type II embryos show asymmetric development of the posterior neural plate and the underlying mesoderm
• Type III embryos fail to develop a normal posterior neural plate
abnormal embryonic neuroepithelial layer differentiation ( J:106144 )
• poor differentiation of the neuroectoderm is seen in areas with increased cell number in the primitive streak

cardiovascular system
abnormal heart looping ( J:106144 )
• embryos with asymmetries often have abnormal cardiac looping

growth/size/body
embryonic growth retardation ( J:106144 )
• at E7.5-E7.5 the Type I embryos show significant developmental delay and remain at the epiblast or early streak stage




Genotype
MGI:3618632
ot8
Allelic
Composition		 Zic3tm1Bca/Y
Genetic
Background		 involves: 129S7/SvEvBrd * Swiss
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zic3tm1Bca mutation (0 available); any Zic3 mutation (37 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
prenatal lethality, incomplete penetrance ( J:75951 )
• significant lethality seen by E10.5 with continued loss of mice through birth
• Background Sensitivity: lethality is more severe in crosses to C57BL/6 compared to outbred Swiss (viability of 0.26 versus 0.46, respectively)




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory