Phenotypes associated with this allele

• Allele Symbol: Fyn^tm1Rmp
• Allele Name: targeted mutation 1, Roger M Perlmutter
• Allele ID: MGI:2179145
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fyn^tm1Rmp/Fyn^tm1Rmp	B6.129S2-Fyn^tm1Rmp	MGI:4360395
hm2	Fyn^tm1Rmp/Fyn^tm1Rmp	involves: 129S2/SvPas	MGI:4360344
hm3	Fyn^tm1Rmp/Fyn^tm1Rmp	involves: 129S2/SvPas * C57BL/6	MGI:4360290
cx4	Fyn^tm1Rmp/Fyn^tm1Rmp Lck^tm1Mak/Lck^tm1Mak	involves: 129S2/SvPas	MGI:4360346

Genotype
MGI:4360395

hm1

• Allelic Composition: Fyn^tm1Rmp/Fyn^tm1Rmp
• Genetic Background: B6.129S2-Fyn^tm1Rmp

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal eosinophil physiology ( J:114287 )

• eosinophil recruitment to the lungs is enhanced when challenged with antigen

decreased IgE level ( J:114287 )

• IgE levels are lower than controls in non-sensitized mice

• upon antigen sensitization, IgE levels are less than half of controls

• upon antigen challenge, IgE levels are similar to controls

increased susceptibility to type I hypersensitivity reaction ( J:114287 )

decreased interleukin-2 secretion ( J:114287 )

• IL-2 secretion is decreased when mitogens are used to stimulate splenocytes

abnormal interleukin-4 secretion ( J:114287 )

• antigen challenge to lungs of sensitized mice leads to 3-fold increase in IL-4 secretion compared to controls

• IL-4 secretion is decreased when mitogens are used to stimulate splenocytes

increased interleukin-5 secretion ( J:114287 )

• antigen challenge to lungs of sensitized mice leads to increased IL-5 secretion

• enhanced secretion is not observed of IL-5 is not observed when mitogens are used to stimulate splenocytes

hematopoietic system

abnormal eosinophil physiology ( J:114287 )

• eosinophil recruitment to the lungs is enhanced when challenged with antigen

decreased IgE level ( J:114287 )

• IgE levels are lower than controls in non-sensitized mice

• upon antigen sensitization, IgE levels are less than half of controls

• upon antigen challenge, IgE levels are similar to controls

Genotype
MGI:4360344

hm2

• Allelic Composition: Fyn^tm1Rmp/Fyn^tm1Rmp
• Genetic Background: involves: 129S2/SvPas

immune system

decreased T cell number ( J:37062 )

• alpha-beta T cell numbers are reduced by little under 2-fold compared to controls

decreased gamma-delta T cell number ( J:37062 )

• gamma-delta T cell numbers are reduced about 2-fold compared to controls

hematopoietic system

decreased T cell number ( J:37062 )

• alpha-beta T cell numbers are reduced by little under 2-fold compared to controls

decreased gamma-delta T cell number ( J:37062 )

• gamma-delta T cell numbers are reduced about 2-fold compared to controls

Genotype
MGI:4360290

hm3

• Allelic Composition: Fyn^tm1Rmp/Fyn^tm1Rmp
• Genetic Background: involves: 129S2/SvPas * C57BL/6

immune system

abnormal T cell activation ( J:70886 )

• CD4 T cells flux less calcium upon TCR stimulation than in controls

decreased T cell proliferation ( J:70886 )

• thymocytes proliferation rate in response to anti-CD3 or ConA stimulation is less than 5% of controls

• splenic T cells also have reduced proliferation though not to the degree as thymocytes

• the proliferative response of T cells to allogenic or super-antigen stimulation is also reduced

abnormal thymocyte activation ( J:70886 )

• single-positive thymocytes have less calcium influx in response to anti-CD3 stimulation

• double-positive thymocyte are largely unaffected in the calcium influx response

hematopoietic system

abnormal T cell activation ( J:70886 )

• CD4 T cells flux less calcium upon TCR stimulation than in controls

decreased T cell proliferation ( J:70886 )

• thymocytes proliferation rate in response to anti-CD3 or ConA stimulation is less than 5% of controls

• splenic T cells also have reduced proliferation though not to the degree as thymocytes

• the proliferative response of T cells to allogenic or super-antigen stimulation is also reduced

abnormal thymocyte activation ( J:70886 )

• single-positive thymocytes have less calcium influx in response to anti-CD3 stimulation

• double-positive thymocyte are largely unaffected in the calcium influx response

endocrine/exocrine glands

abnormal thymocyte activation ( J:70886 )

• single-positive thymocytes have less calcium influx in response to anti-CD3 stimulation

• double-positive thymocyte are largely unaffected in the calcium influx response

cellular

decreased T cell proliferation ( J:70886 )

• thymocytes proliferation rate in response to anti-CD3 or ConA stimulation is less than 5% of controls

• splenic T cells also have reduced proliferation though not to the degree as thymocytes

• the proliferative response of T cells to allogenic or super-antigen stimulation is also reduced

Genotype
MGI:4360346

cx4

• Allelic Composition: Fyn^tm1Rmp/Fyn^tm1Rmp; Lck^tm1Mak/Lck^tm1Mak
• Genetic Background: involves: 129S2/SvPas

immune system

thymus hypoplasia ( J:37062 , J:37940 )

• thymus cellularity is 1-5% of normal (J:37062)

• thymic cellularity is 50- to 100-fold less than controls (J:37940)

decreased double-positive T cell number ( J:37940 )

• double-positive thymocytes are virtually absent

arrested T cell differentiation ( J:37062 , J:37940 )

• T cell development is blocked in the transition from double-negative to double-positive stage (J:37062)

• the thymocytes are predominately CD25⁺ and HAS^hi (J:37062)

• T cell development is arrested at the CD44^-CD25⁺ stage with 85-92% of thymocytes being at this stage (J:37940)

absent CD4-positive, alpha-beta T cells ( J:37940 )

• CD4 T cells are virtually absent from the periphery

absent CD8-positive, alpha-beta T cells ( J:37940 )

• CD8 T cells are virtually absent from the periphery

abnormal NK T cell number ( J:37940 )

• NK1.1⁺ alpha-beta T cells are virtually absent from the periphery

decreased gamma-delta intraepithelial T cell number ( J:37940 , J:112977 )

• dendritic epithelial T cell numbers are severely reduced in the skin (J:37940)

• CD8alpha-alpha gamma-delta IEL are present but in smaller numbers (J:112977)

absent T cells ( J:37062 )

• alpha-beta and gamma-delta T cells are absent in these mice

abnormal alpha-beta intraepithelial T cell morphology ( J:112977 )

• alpha-beta IEL are absent in these mice

• gamma-delta IEL are still present

hematopoietic system

thymus hypoplasia ( J:37062 , J:37940 )

• thymus cellularity is 1-5% of normal (J:37062)

• thymic cellularity is 50- to 100-fold less than controls (J:37940)

decreased double-positive T cell number ( J:37940 )

• double-positive thymocytes are virtually absent

arrested T cell differentiation ( J:37062 , J:37940 )

• T cell development is blocked in the transition from double-negative to double-positive stage (J:37062)

• the thymocytes are predominately CD25⁺ and HAS^hi (J:37062)

• T cell development is arrested at the CD44^-CD25⁺ stage with 85-92% of thymocytes being at this stage (J:37940)

absent CD4-positive, alpha-beta T cells ( J:37940 )

• CD4 T cells are virtually absent from the periphery

absent CD8-positive, alpha-beta T cells ( J:37940 )

• CD8 T cells are virtually absent from the periphery

abnormal NK T cell number ( J:37940 )

• NK1.1⁺ alpha-beta T cells are virtually absent from the periphery

decreased gamma-delta intraepithelial T cell number ( J:37940 , J:112977 )

• dendritic epithelial T cell numbers are severely reduced in the skin (J:37940)

• CD8alpha-alpha gamma-delta IEL are present but in smaller numbers (J:112977)

absent T cells ( J:37062 )

• alpha-beta and gamma-delta T cells are absent in these mice

abnormal alpha-beta intraepithelial T cell morphology ( J:112977 )

• alpha-beta IEL are absent in these mice

• gamma-delta IEL are still present

endocrine/exocrine glands

thymus hypoplasia ( J:37062 , J:37940 )

• thymus cellularity is 1-5% of normal (J:37062)

• thymic cellularity is 50- to 100-fold less than controls (J:37940)