Phenotypes associated with this allele

• Allele Symbol: Gck^tm1.1Mgn
• Allele Name: targeted mutation 1.1, Mark A Magnuson
• Allele ID: MGI:2177709
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gck^tm1.1Mgn/Gck^tm1.1Mgn	129S6/SvEvTac-Gck^tm1.1Mgn	MGI:3590683
hm2	Gck^tm1.1Mgn/Gck^tm1.1Mgn	involves: 129S6/SvEvTac * C57BL/6	MGI:3590680
cn3	Gck^tm1.1Mgn/Gck^tm1.1Mgn Tg(Ins2-cre)25Mgn/0	involves: 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:3603003
cn4	Gck^tm1.1Mgn/Gck^+ Tg(Ins2-cre)25Mgn/0	involves: 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:3603012
cn5	Gck^tm1.1Mgn/Gck^tm1.1Mgn Speer6-ps1^Tg(Alb-cre)21Mgn/Speer6-ps1^+	involves: 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:3603013

Genotype
MGI:3590683

hm1

• Allelic Composition: Gck^tm1.1Mgn/Gck^tm1.1Mgn
• Genetic Background: 129S6/SvEvTac-Gck^tm1.1Mgn

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

increased circulating glucose level ( J:51826 )

• slight increase in blood glucose concentration (165 mg/dl vs. 132 mg/dl in wild-type)

Genotype
MGI:3590680

hm2

• Allelic Composition: Gck^tm1.1Mgn/Gck^tm1.1Mgn
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

homeostasis/metabolism

increased circulating glucose level ( J:51826 )

• about 10% increase in blood glucose concentration (194 mg/dl vs. 175 mg/dl in wild-type)

Genotype
MGI:3603003

cn3

• Allelic Composition: Gck^tm1.1Mgn/Gck^tm1.1Mgn; Tg(Ins2-cre)25Mgn/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * DBA/2

mortality/aging

neonatal lethality, incomplete penetrance ( J:51826 )

• more than 80% neonatal mortality as a result of severe hyperglycemia

homeostasis/metabolism

hyperglycemia ( J:51826 )

• highly variable but increased blood glucose concentrations

decreased circulating insulin level ( J:51826 )

• decreased by about 70%

decreased liver glycogen level ( J:51826 )

• diminished hepatic glycogen

liver/biliary system

decreased liver glycogen level ( J:51826 )

• diminished hepatic glycogen

hepatic steatosis ( J:51826 )

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
maturity-onset diabetes of the young type 2		DOID:0111100	OMIM:125851	J:51826

Genotype
MGI:3603012

cn4

• Allelic Composition: Gck^tm1.1Mgn/Gck⁺; Tg(Ins2-cre)25Mgn/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * DBA/2

homeostasis/metabolism

decreased insulin secretion ( J:51826 )

• 70% decrease in insulin secretion during hyperglycemic clamp studies

hyperglycemia ( J:51826 )

• blood glucose concentration is increased by about 50% compared to heterozygous Gck^tm1.1Mgn mice

• under fasting conditions, have a 25% increase in blood glucose concentration, without differences in basal insulin concentration

impaired glucose tolerance ( J:51826 )

• glucose turnover and glucose infusion rates during hyperglycemic clamp are reduced by about 60 and 70%, respectively

decreased glycogen level ( J:51826 )

• net glycogen synthesis in liver is reduced by about 50% during hyperglycemic clamp studies

endocrine/exocrine glands

decreased insulin secretion ( J:51826 )

• 70% decrease in insulin secretion during hyperglycemic clamp studies

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
maturity-onset diabetes of the young type 2		DOID:0111100	OMIM:125851	J:51826

Genotype
MGI:3603013

cn5

• Allelic Composition: Gck^tm1.1Mgn/Gck^tm1.1Mgn; Speer6-ps1^{Tg(Alb-cre)21Mgn}/Speer6-ps1⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * DBA/2

homeostasis/metabolism

abnormal glucose homeostasis ( J:51826 )

decreased insulin secretion ( J:51826 )

• secrete 70% less insulin in response to glucose stimulus

increased circulating glucose level ( J:51826 )

• 10% increase in fed blood glucose concentration

increased circulating insulin level ( J:51826 )

• post-absorptive (fed) plasma insulin concentrations twice that of controls

impaired glucose tolerance ( J:51826 )

• glucose turnover and glucose infusion rates during hyperglycemic clamp are reduced

decreased glycogen level ( J:51826 )

• net hepatic glycogen synthesis is reduced by about 90% during hyperglycemic clamp studies

insulin resistance ( J:51826 )

• mild insulin resistance

endocrine/exocrine glands

decreased insulin secretion ( J:51826 )

• secrete 70% less insulin in response to glucose stimulus

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
maturity-onset diabetes of the young type 2		DOID:0111100	OMIM:125851	J:51826