Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Npr3tm1Unc mutation
(1 available);
any
Npr3 mutation
(47 available)
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adipose tissue
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• in epididymal white adipose tissue
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• no superficial white adipose tissue and a deeper reddish-brown tint and reduced lipid droplets
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• primary adipocytes exhibit elevated glycerol release in response to atrial natriuretic peptides unlike wild-type cells
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Npr3tm1Unc mutation
(1 available);
any
Npr3 mutation
(47 available)
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Skeletal phenotypes of Npr3tm1Unc/Npr3tm1Unc mice
mortality/aging
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• homozygotes develop to term; however, about 50% die before weaning
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hematopoietic system
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• homozygotes display progressively increased red blood cell counts relative to wild-type mice (10.4 0.1 106/mm3 vs 9.7 0.1 106/mm3, respectively)
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• homozygotes display progressively increased hematocrits relative to wild-type mice (53.6 0.9 vs 49.9 1.0, respectively)
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• homozygotes display progressively increased hemoglobin levels relative to wild-type mice (16.7 0.2 g/dl vs 15.6 0.2 g/dl, respectively)
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• the number and size of osteoclasts in cultures of bone marrow cells from 2-month-old homozygotes are ~2x that of wild-type
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homeostasis/metabolism
cardiovascular system
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• homozygotes exhibit reduced intravascular blood volume relative to wild-type mice
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• in homozygotes, blood pressures are reduced by 8 mmHg relative to wild-type mice (110.4 2.3 mmHg vs 118.7 1.9 mmHg, respectively)
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renal/urinary system
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• homozygotes exhibit a >300% increase in total daily excretion of cGMP in the urine relative to wild-type mice
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• homozygotes can excrete dilute urine after water loading but are progressively and significantly less able than wild-type mice to concentrate urine; however, no shortening of the renal papilla is observed
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• homozygotes exhibit no significant differences in plasma Na+, K+, Ca2+, Cl-, creatinine, and urea nitrogen concentrations or in daily urinary excretion of Na+, K+, Cl-, creatinine, or protein relative to wild-type mice
• however, homozygotes display a mild progressive increase in total daily urine output relative to wild-type littermates
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behavior/neurological
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• homozygotes tend to display a progressive increase in water intake
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• at P7, homozygotes display hunched backs
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growth/size/body
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• at P7, homozygotes display decreased body weight relative to wild-type mice
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• at P7, homozygotes display increased body length relative to wild-type mice
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skeleton
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• at P7, homozygotes display dome-shaped skulls
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• the number and size of osteoclasts in cultures of bone marrow cells from 2-month-old homozygotes are ~2x that of wild-type
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• at P7, homozygotes display elongated femurs and tibias relative to wild-type mice
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• at P7, homozygotes display elongated metatarsal and digital bones relative to wild-type mice
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• adult homozygotes exhibit small thoracic cages relative to wild-type mice
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• at P7, homozygotes display longer vertebral bodies than wild-type mice
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• the number of osteoblastic precursors from 2-month-old homozygotes is ~3 that of wild-type, and they proliferate almost twice as rapidly
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• in young homozygotes, bony trabeculae appear thicker and longer than normal
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• at P10, cellular expansion is apparent in the zone of hypertrophic chondrocytes but not in the zones with resting or proliferating chondrocytes; however, this difference is no longer observed at 3 months
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• at P10, homozygotes display delayed development of secondary ossification centers in their long bones
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• at 2 months, homozygotes (but not heterozygotes) exhibit increased bone turnover, as shown by a 150% increase in plasma alkaline phosphatase (an indicator of bone formation) and a >200% increase in urinary pyridonoline and deoxypyridonoline (indicators of bone resorption)
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craniofacial
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• at P7, homozygotes display dome-shaped skulls
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limbs/digits/tail
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• at P7, homozygotes display elongated metatarsal and digital bones relative to wild-type mice
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• at P7, homozygotes exhibit elongated tails that, in some mice, appear initially wavy
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reproductive system
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• only one third of surviving homozygotes reproduce at least once
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immune system
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• the number and size of osteoclasts in cultures of bone marrow cells from 2-month-old homozygotes are ~2x that of wild-type
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Allelic
Composition |
Npr3tm1Unc/Npr3+
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Genetic
Background |
involves: 129 * C57BL/6 |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Npr3tm1Unc mutation
(1 available);
any
Npr3 mutation
(47 available)
|
|
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hematopoietic system
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• heterozygotes display progressively increased red blood cell counts relative to wild-type mice (10.1 0.3 106/mm3 vs 9.7 0.1 106/mm3, respectively)
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• heterozygotes display progressively increased hematocrits relative to wild-type mice (51.8 0.9 vs 49.9 1.0, respectively)
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• heterozygotes display progressively increased hemoglobin levels relative to wild-type mice (16.2 0.2 g/dl vs 15.6 0.2 g/dl, respectively)
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homeostasis/metabolism
cardiovascular system
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• heterozygotes exhibit reduced intravascular blood volume; however, no reduction in blood pressure is observed relative to wild-type mice
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renal/urinary system
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• homozygotes exhibit a >200% increase in total daily excretion of cGMP in the urine relative to wild-type mice
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• heterozygotes can excrete dilute urine after water loading but become progressively and significantly less able to concentrate urine relative to wild-type mice
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• similar to homozygotes, heterozygotes show a mild progressive increase in total daily urine output relative to wild-type littermates
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Analysis Tools