Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ccr4tm1Pwr mutation
(1 available);
any
Ccr4 mutation
(31 available)
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immune system
hematopoietic system
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ccr4tm1Pwr mutation
(1 available);
any
Ccr4 mutation
(31 available)
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immune system
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• in vitro, homozygotes display normal Th2 and Th1 cell differentiation relative to wild-type mice
• in an OVA-induced model of airway inflammation, homozygotes display normal Th2-associated bronchial hyperreactivity in response to inhaled methacholine after OVA priming and intranasal challenge with saline, with no differences in OVA-induced eosinophilia in BALF and lung tissue, in total cell count or individual leukocyte populations in BALF, or in OVA-specific Ig titers relative to wild-type mice
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• after A. fumigatus infection in the bronchoalveolar lavage
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• after A. fumigatus infection in the bronchoalveolar lavage
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• at 24 hrs after high dose LPS treatment, homozygotes show a reduction in the number of lymphocytes in the peritoneal lavage relative to wild-type mice
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• at 24 hrs after high dose LPS treatment, homozygotes display significantly fewer macrophages in the peritoneal lavage, with reduced numbers of CD11b+ and CD14+ cells and an absence or severely reduced number of F4/80-expressing cells relative to wild-type mice
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• after A. fumigatus infection in the bronchoalveolar lavage
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• 30 days after A. fumigatus infection
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• 3 and 7 days after A. fumigatus infection
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• 3 days after A. fumigatus infection
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• unexpectedly, mutant splenocytes show no chemotactic response to human or mouse chemokine (C-C motif) ligand 3 (also known as macrophage inflammatory protein MIP-1alpha)
• in contrast, the response of mutant splenocytes to human RANTES is similar to that observed in wild-type mice
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• at 3 hrs after injection of a high dose of LPS (60 mg/kg), homozygotes show a significant decrease in serum MIP-alpha levels relative to wild-type mice, suggesting a possible macrophage defect
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• 3 days after A. fumigatus infection
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• at 3 hrs after injection of a high dose of LPS (60 mg/kg), homozygotes show a 6-fold decrease in serum IL-1beta levels relative to wild-type mice
• in contrast, IL-6 production remains unchanged relative to wild-type mice
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• 30 days after A. fumigatus infection
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• 3 days after A. fumigatus infection
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• 30 days after A. fumigatus infection
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• 30 days after A. fumigatus infection
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• 3 days after A. fumigatus infection
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• 30 days after A. fumigatus infection
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• 3 days after A. fumigatus infection
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• in response to a high LPS dose (60 mg/kg), homozygotes fail to induce significant levels of serum TNF, whereas wild-type mice show a sharp increase in serum TNF levels at 1.5 hrs which return to baseline by 4 hrs after LPS treatment
• in vitro, peritoneal lavage cells from naive homozygotes produce significantly lower TNF levels when cultured for 18 hrs in the presence of 1 g/ml LPS relative to cells from naive wild-type mice (107 11 versus 201 18 pg/ml, respectively)
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• after A. fumigatus infection
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• in response to high doses of LPS (60-120 mg/kg), homozygotes show no differences in initial platelet mobilization but display milder signs of shivering and lethargy than wild-type during the first few hours after LPS injection, with platelet counts returning to normal at day 5 and 93% of homozygotes still alive on day 6, whereas all wild-type die between 2 and 4 days after treatment
• in response to a low dose of LPS (1 g) plus 8 mg of D-gal, 9 of 12 homozygotes survive whereas the majority of wild-type (11 of 12) die; following treatment with 4 g of LPS plus D-gal, all homozygotes tested die with a 6-hr delay relative to wild-type mice
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• 7 days after Aspergillus fumigatus infection, mice clear fungal spores from the lungs; increased neutrophil, eosinophil, and macrophage numbers in the bronchoalveolar lavage (BAL); increased early airway resistance; increased early levels of TNFalpha, IFN-gamma, IL4, IL5, and IL10 in the BAL; and increased early IgE and IgG1 levels compared with wild-type mice
• 3 days after A. fumigatus infection airway resistance is increased compared to in wild-type mice
• 30 days after A. fumigatus infection airway resistance is decreased compared to in wild-type mice
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respiratory system
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• 30 days after A. fumigatus infection
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• 3 days after A. fumigatus infection
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hematopoietic system
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• after A. fumigatus infection in the bronchoalveolar lavage
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• after A. fumigatus infection in the bronchoalveolar lavage
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• at 24 hrs after high dose LPS treatment, homozygotes show a reduction in the number of lymphocytes in the peritoneal lavage relative to wild-type mice
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• at 24 hrs after high dose LPS treatment, homozygotes display significantly fewer macrophages in the peritoneal lavage, with reduced numbers of CD11b+ and CD14+ cells and an absence or severely reduced number of F4/80-expressing cells relative to wild-type mice
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• after A. fumigatus infection in the bronchoalveolar lavage
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• 30 days after A. fumigatus infection
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• 3 and 7 days after A. fumigatus infection
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• 3 days after A. fumigatus infection
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• unexpectedly, mutant splenocytes show no chemotactic response to human or mouse chemokine (C-C motif) ligand 3 (also known as macrophage inflammatory protein MIP-1alpha)
• in contrast, the response of mutant splenocytes to human RANTES is similar to that observed in wild-type mice
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Analysis Tools