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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Ccr5tm1Kuz
targeted mutation 1, William A Kuziel
MGI:1861911
Summary 8 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Ccr5tm1Kuz/Ccr5tm1Kuz B6.129P2-Ccr5tm1Kuz MGI:4418655
hm2
Ccr5tm1Kuz/Ccr5tm1Kuz B6.129P2-Ccr5tm1Kuz/J MGI:6460298
hm3
Ccr5tm1Kuz/Ccr5tm1Kuz involves: 129P2/OlaHsd MGI:5774773
hm4
Ccr5tm1Kuz/Ccr5tm1Kuz involves: 129P2/OlaHsd * C57BL/6 MGI:3609170
ht5
Ccr5tm1Kuz/Ccr5+ involves: 129P2/OlaHsd MGI:5317841
cx6
Ccr5tm1Kuz/Ccr5tm1Kuz
Cxcr4tm1Qma/Cxcr4tm1Qma
involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 MGI:5317843
cx7
Ccr5tm1Kuz/Ccr5tm1Kuz
Tg(CAG-EGFP)1Osb/?
involves: 129P2/OlaHsd * C3H * C57BL/6 MGI:5317844
cx8
Apoetm1Unc/Apoetm1Unc
Ccr5tm1Kuz/Ccr5tm1Kuz
involves: 129P2/OlaHsd * C57BL/6 MGI:5317842


Genotype
MGI:4418655
hm1
Allelic
Composition
Ccr5tm1Kuz/Ccr5tm1Kuz
Genetic
Background
B6.129P2-Ccr5tm1Kuz
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ccr5tm1Kuz mutation (2 available); any Ccr5 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
respiratory system
• mice infected with a recombinant mouse-adapted SARS-CoV, rMA15, develop more severe denuding bronchiolitis than similarly infected wild-type mice

behavior/neurological
N
• response to kainic acid similar to controls
• become catatonic and display staring behavior within 15 minutes
• myoclonic twitching, frequent rearing and falling
• seizures lasting up to 5 hours
• female mice develop a stronger ethanol-induced conditioned taste aversion compared with similarly treated wild-type mice
• of an ethanol, saccharin, or quinine solution
• in female but not male mice

immune system
• mice infected with a recombinant mouse-adapted SARS-CoV, rMA15, develop more severe denuding bronchiolitis than similarly infected wild-type mice
• mice show increased susceptibility to infection with a recombinant mouse-adapted SARS-CoV, rMA15 , developing more severe and prolonged disease compared to wild-type mice, showing more prominent airway epithelial cell apoptosis, severe denuding bronchiolitis and perivenular/periarterial cuffing

mortality/aging
N
• 40% mortality after kainic acid treatment

nervous system
N
• selective CA3 hippocampal pycnosis after kainic acid treatment as in controls
• susceptibility of cerebral granule cell neurons to kainic acid is similar to controls

vision/eye
• 34% less neovascularization than controls 2 weeks after denudation of corneal epithelium
• 34.9% less neovascularization than controls 4 weeks after denudation of corneal epithelium

cardiovascular system
• 34% less neovascularization than controls 2 weeks after denudation of corneal epithelium
• 34.9% less neovascularization than controls 4 weeks after denudation of corneal epithelium

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
Coronavirus infectious disease DOID:0080599 J:162707




Genotype
MGI:6460298
hm2
Allelic
Composition
Ccr5tm1Kuz/Ccr5tm1Kuz
Genetic
Background
B6.129P2-Ccr5tm1Kuz/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ccr5tm1Kuz mutation (2 available); any Ccr5 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• in a forced swimming test, mutant mice show a small but significant decrease in swimming time relative to wild-type controls
• administration of ORM1 fails to increase swimming time, unlike in wild-type controls, indicating that CCR5 mediates the anti-fatigue effect of ORM1

homeostasis/metabolism
• in a forced swimming test, mutant mice show a small but significant decrease in swimming time relative to wild-type controls
• administration of ORM1 fails to increase swimming time, unlike in wild-type controls, indicating that CCR5 mediates the anti-fatigue effect of ORM1
• mutant mice show a small but significant decrease in skeletal muscle glycogen content relative to wild-type controls
• administration of ORM1 fails to increase muscle glycogen content, unlike in wild-type controls

muscle
• mutant mice show a small but significant decrease in skeletal muscle glycogen content relative to wild-type controls
• administration of ORM1 fails to increase muscle glycogen content, unlike in wild-type controls




Genotype
MGI:5774773
hm3
Allelic
Composition
Ccr5tm1Kuz/Ccr5tm1Kuz
Genetic
Background
involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ccr5tm1Kuz mutation (2 available); any Ccr5 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• after hypoxia exposure for 18 days, mice show lower right ventricular systolic pressure, less severe right ventricular hypertrophy, less muscularization of the distal pulmonary vessels with a smaller percentage of dividing Ki67-positive pulmonary vascular cells, lower counts of lung inflammatory monocytes and lower number of macrophages surrounding pulmonary vessels or present in the BAL fluid

cardiovascular system
• after hypoxia exposure for 18 days, right ventricular hypertrophy is less severe in mutants than in wild-type mice
• after hypoxia exposure for 18 days, distal pulmonary vessels exhibit less muscularization than wild-type mice, with a smaller percentage of dividing Ki67-positive pulmonary vascular cells
• after hypoxia exposure for 18 days, right ventricular systolic pressure is lower in mutants than in wild-type mice

immune system
• the number of macrophages surrounding pulmonary vessels or present in BAL fluid is not increased from normoxia to hypoxia as is seen in wild-type mice
• mice show lower counts of lung inflammatory monocytes than wild-type mice during both normoxia and hypoxia




Genotype
MGI:3609170
hm4
Allelic
Composition
Ccr5tm1Kuz/Ccr5tm1Kuz
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ccr5tm1Kuz mutation (2 available); any Ccr5 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• infiltration of Kupffer cells an NK cells is strongly reduced after bile duct ligation
• mediates liver fibrosis primarily through resident liver cells
• 80% fewer macrophages compared to control in Ag-loaded gelatin sponge DTH assay (J:64293)
• delay in induced peritoneal macrophage accumulation, however, accumulation of neutrophils/eosinophils is unchanged (J:84243)
• reduced chemotaxis response (J:153059)
• null mice show clinically less severe DSS-mediated colitis than wild-type or Ccr2-nulls
• 4 fold reduction of tumor infiltrating NK cells when mice are injected with B16 melanoma cells
• null mice show a significant increase in NK 1.1+ T cells by day 3 and 7 of DSS-treatment; wild-type and Ccr2-null mice do not show as large of an increase
• intestinal mucosa shows enhanced infiltration of CD4+ lymphocytes by day 7 compared to wild-type or Ccr2-deficient mice
• mice have an increased number of CD4+ T cells before and during DSS-induced colitis
• increased resistance to activation induced cell death
• increased numbers of Il4 producing cells
• increased FasL expressing cells
• significant reduction in activated microglia in the hippocampus 8 hours after injection of beta amyloid 1-40
• mice expression one fifth the wild-type level of Ifng after day 3 and 7, while Il-4 is increased 75-fold and a 2-fold increase in Il-5 and -10 over wild-type
• reduced survival following inoculation with Cryptococcus neoformans, 40% die by week 8 due to CNS infection (hydrocephalus)
• infected mice exhibit increased mononuclear cell recruitment to lungs, but not brain
• by week 5 infected mice exhibit cryptococcal meningitis cranial swelling, ruffled fur, staggered gait, lethargy and unresponsiveness, and decreased limb function
• brains of infected mice develop loss of neural tissue integrity , and large amounts of extracellelular cryptococcal polysaccharide capsule
• abrogated neurotoxicity to gp120 from a CCR5 preferring isolate of HIV
• abrogated neurotoxicity to gp120 from a CCR4 preferring isolate of HIV

behavior/neurological
• improved learning performance in a Morris water maze after injection of beta amyloid 1-40 relative to controls
• mice show little reduction in activity with DSS treatment, unlike wild-type which become very lethargic with time

digestive/alimentary system
• null mice show clinically less severe DSS-mediated colitis than wild-type or Ccr2-nulls

hematopoietic system
• infiltration of Kupffer cells an NK cells is strongly reduced after bile duct ligation
• mediates liver fibrosis primarily through resident liver cells
• 80% fewer macrophages compared to control in Ag-loaded gelatin sponge DTH assay (J:64293)
• delay in induced peritoneal macrophage accumulation, however, accumulation of neutrophils/eosinophils is unchanged (J:84243)
• reduced chemotaxis response (J:153059)
• 4 fold reduction of tumor infiltrating NK cells when mice are injected with B16 melanoma cells
• null mice show a significant increase in NK 1.1+ T cells by day 3 and 7 of DSS-treatment; wild-type and Ccr2-null mice do not show as large of an increase
• intestinal mucosa shows enhanced infiltration of CD4+ lymphocytes by day 7 compared to wild-type or Ccr2-deficient mice
• mice have an increased number of CD4+ T cells before and during DSS-induced colitis
• increased FasL expressing cells
• increased resistance to activation induced cell death
• increased numbers of Il4 producing cells
• significant reduction in activated microglia in the hippocampus 8 hours after injection of beta amyloid 1-40

homeostasis/metabolism
• mice expression one fifth the wild-type level of Ifng after day 3 and 7, while Il-4 is increased 75-fold and a 2-fold increase in Il-5 and -10 over wild-type

cellular
• 80% fewer macrophages compared to control in Ag-loaded gelatin sponge DTH assay (J:64293)
• delay in induced peritoneal macrophage accumulation, however, accumulation of neutrophils/eosinophils is unchanged (J:84243)
• reduced chemotaxis response (J:153059)

nervous system
• abrogated neurotoxicity to gp120 from a CCR5 preferring isolate of HIV
• abrogated neurotoxicity to gp120 from a CCR4 preferring isolate of HIV
• significant reduction in activated microglia in the hippocampus 8 hours after injection of beta amyloid 1-40
• significant reduction in activated astrocytes in the hippocampus 6 hours after injection of beta amyloid 1-40

cardiovascular system
• infiltration of Kupffer cells an NK cells is strongly reduced after bile duct ligation
• mediates liver fibrosis primarily through resident liver cells

liver/biliary system
• infiltration of Kupffer cells an NK cells is strongly reduced after bile duct ligation
• mediates liver fibrosis primarily through resident liver cells
• complete suppression of hepatic stellate cell migration
• injection of Concanavalin A results in 25% increase in damage
• hepatocellular necrosis
• injection of Concanavalin A leads to fulminant liver failure and 50% mortality within 8 hours whereas all controls survive
• antibody depletion of NKT cells improves liver damage
• IL4 depletion improves liver damage




Genotype
MGI:5317841
ht5
Allelic
Composition
Ccr5tm1Kuz/Ccr5+
Genetic
Background
involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ccr5tm1Kuz mutation (2 available); any Ccr5 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
N
• normal pregnancies




Genotype
MGI:5317843
cx6
Allelic
Composition
Ccr5tm1Kuz/Ccr5tm1Kuz
Cxcr4tm1Qma/Cxcr4tm1Qma
Genetic
Background
involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ccr5tm1Kuz mutation (2 available); any Ccr5 mutation (25 available)
Cxcr4tm1Qma mutation (1 available); any Cxcr4 mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• abrogated neurotoxicity to gp120 from a CCR5 preferring isolate of HIV
• abrogated neurotoxicity to gp120 from a CCR4 preferring isolate of HIV

immune system
• abrogated neurotoxicity to gp120 from a CCR5 preferring isolate of HIV
• abrogated neurotoxicity to gp120 from a CCR4 preferring isolate of HIV




Genotype
MGI:5317844
cx7
Allelic
Composition
Ccr5tm1Kuz/Ccr5tm1Kuz
Tg(CAG-EGFP)1Osb/?
Genetic
Background
involves: 129P2/OlaHsd * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ccr5tm1Kuz mutation (2 available); any Ccr5 mutation (25 available)
Tg(CAG-EGFP)1Osb mutation (7 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
neoplasm
• 40.2 metastic colonies compared to 70.6 colonies for controls 14 days after injecting B16-F10 melanoma cells
• fewer metastatic tumors 14 days after injecting irradiated mice with melanoma cells
• injection of wild type lung stromal cells enhanced metastasis relative to injection of mutant stromal cells
• improved survival




Genotype
MGI:5317842
cx8
Allelic
Composition
Apoetm1Unc/Apoetm1Unc
Ccr5tm1Kuz/Ccr5tm1Kuz
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apoetm1Unc mutation (33 available); any Apoe mutation (139 available)
Ccr5tm1Kuz mutation (2 available); any Ccr5 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• reduced plaque areas after 10-12 weeks on a high fat or high cholesterol diet
• both in the aortic root and the thoracoabdominal artery
• also at 22 weeks when fed a normal diet
• increased smooth muscle content in atherosclerotic plaques

immune system
• in atherosclerotic plaques reduced 24% compared to Apoetm1Unc
• monocyte/macrophage in atherosclerotic plaques reduced 77% compared to Apoetm1Unc
• reduced proliferative responses of splenocytes and of lymph node cells by 25% compared to Apoetm1Unc
• significantly decreased secretion by splenocytes
• increased content in atherosclerotic plaques
• increased secretion by both splenocytes and by lymph node cells

muscle
• increased smooth muscle content in atherosclerotic plaques

hematopoietic system
• in atherosclerotic plaques reduced 24% compared to Apoetm1Unc
• monocyte/macrophage in atherosclerotic plaques reduced 77% compared to Apoetm1Unc
• reduced proliferative responses of splenocytes and of lymph node cells by 25% compared to Apoetm1Unc





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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
01/17/2023
MGI 6.22
The Jackson Laboratory