Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxa2tm1Jrt mutation
(2 available);
any
Foxa2 mutation
(26 available)
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embryo
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• A-P axis appears to be aligned incorrectly and the anterior pole of the axis remains in the distal region of the embryo
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• absence of a distinct node
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• the streak is incorrectly positioned in the proximal region of the epiblast
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• primitive streak does not extend the full length of the posterior side of the embryo
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxa2tm1Jrt mutation
(2 available);
any
Foxa2 mutation
(26 available)
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cellular
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• embryos exhibit an increase in overall level of cell death; cell death is higher in the region distal to the primitive streak than in the proximal region of the E6.5 embryo
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embryo
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• embryos exhibit an increase in overall level of cell death; cell death is higher in the region distal to the primitive streak than in the proximal region of the E6.5 embryo
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxa2tm1Jrt mutation
(2 available);
any
Foxa2 mutation
(26 available)
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mortality/aging
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• die between E9.5 and E11.5
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embryo
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• embryos exhibit an increase in overall level of cell death; cell death is higher in the region distal to the primitive streak than in the proximal region of the E6.5 embryo
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• at E9.5 in less severely affected embryos the anterior structures are either misshapen or truncated
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• at E9.5 in less severely affected embryos the anterior structures may be truncated
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• by E7.5 the embryonic tissues are reduced in size and abnormally shaped
• at E8.5 in severely affected embryos only a small structure links the posterior end of the embryo to the exocoelom and few embryonic tissues are recognizable
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• at E6.5 primitive endoderm cells accumulate at the distal tip of the embryo and these cells appear rough and dull
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• in less severely affected embryos the anterior neural tube is abnormal
• dorso-ventral patterning of the neural tube is abnormal
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• in less severely affected embryos the posterior neural tube is kinked
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• at E7.5 no definitive node structure is detected
• cells expressing Gsc fail to migrate to the node
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• at E7.5 only the region of the primitive streak closest to the embryonic extraembryonic tissue boundary is present
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• in less severely affected embryos the somites are irregularly shaped
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• in less severely affected embryos the somites are small
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• in less severely affected embryos the somites are often fused at the midline
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• at E6.5 an abnormal constriction between the embryonic and extraembryonic tissue is seen
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nervous system
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• in less severely affected embryos the anterior neural tube is abnormal
• dorso-ventral patterning of the neural tube is abnormal
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• in less severely affected embryos the posterior neural tube is kinked
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digestive/alimentary system
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• at E8.5 no signs of gut development are seen however by E9.5 a closed gut like structure is seen ventral to the spinal cord in the trunk region of some embryos
• at E9.5 in the anterior portion of the embryo cell expressing gut markers are found on the outside rather than the inside of the embryo suggesting failure of invagination
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cellular
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• embryos exhibit an increase in overall level of cell death; cell death is higher in the region distal to the primitive streak than in the proximal region of the E6.5 embryo
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Allelic
Composition |
Foxa2tm1Jrt/Foxa2+
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Genetic
Background |
involves: 129S1/Sv * 129X1/SvJ |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxa2tm1Jrt mutation
(2 available);
any
Foxa2 mutation
(26 available)
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mortality/aging
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• some of those that survive until weaning die by 3 months of age
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• Background Sensitivity: loss prior to weaning is higher on a mixed 129 and C57BL/6 background than on a 129 background
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craniofacial
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• overgrowth of the upper and lower incisors is seen in mice with malocclusion
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• malocclusion of the jaws is seen in those that die by 3 months of age
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behavior/neurological
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• seen in those that die by 3 months of age
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• seen in those that die by 3 months of age
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growth/size/body
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• overgrowth of the upper and lower incisors is seen in mice with malocclusion
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• malocclusion of the jaws is seen in those that die by 3 months of age
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• seen in those that die by 3 months of age
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skeleton
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• overgrowth of the upper and lower incisors is seen in mice with malocclusion
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• malocclusion of the jaws is seen in those that die by 3 months of age
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Allelic
Composition |
Foxa2tm1Jrt/Foxa2+
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Genetic
Background |
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxa2tm1Jrt mutation
(2 available);
any
Foxa2 mutation
(26 available)
|
|
|
mortality/aging
|
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• some of those that survive until weaning die by 3 months of age
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• Background Sensitivity: loss prior to weaning is higher on a mixed 129 and C57BL/6 background than on a 129 background
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craniofacial
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• overgrowth of the upper and lower incisors is seen in mice with malocclusion
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• malocclusion of the jaws is seen in those that die by 3 months of age
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behavior/neurological
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• seen in those that die by 3 months of age
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• seen in those that die by 3 months of age
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growth/size/body
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• overgrowth of the upper and lower incisors is seen in mice with malocclusion
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• malocclusion of the jaws is seen in those that die by 3 months of age
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• seen in those that die by 3 months of age
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skeleton
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• overgrowth of the upper and lower incisors is seen in mice with malocclusion
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• malocclusion of the jaws is seen in those that die by 3 months of age
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Allelic
Composition |
Foxa2tm1Jrt/Foxa2+
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Genetic
Background |
involves: 129S1/Sv * 129X1/SvJ * CD-1 |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxa2tm1Jrt mutation
(2 available);
any
Foxa2 mutation
(26 available)
|
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mortality/aging
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• about 20% of those that survive until weaning die by 3 months of age and display abnormalities
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• Background Sensitivity: loss prior to weaning is higher on a mixed 129 and C57BL/6 background than on a 129 background or mixed 129 and CD-1 background
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craniofacial
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• overgrowth of the upper and lower incisors is seen in mice with malocclusion
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• malocclusion of the jaws is seen in those that die by 3 months of age
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behavior/neurological
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• seen in those that die by 3 months of age
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• seen in those that die by 3 months of age
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growth/size/body
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• overgrowth of the upper and lower incisors is seen in mice with malocclusion
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• malocclusion of the jaws is seen in those that die by 3 months of age
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• seen in those that die by 3 months of age
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skeleton
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• overgrowth of the upper and lower incisors is seen in mice with malocclusion
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• malocclusion of the jaws is seen in those that die by 3 months of age
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mortality/aging
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• mutant embryos die at E11.5
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embryo
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• the axial mesendoderm failed to differentiate; the anterior neural plate and anterior definitive endoderm form, but fail to maintain specification
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nervous system
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• anterior head truncations were noted in mutant animals
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxa2tm1Jrt mutation
(2 available);
any
Foxa2 mutation
(26 available)
Gsctm1Bhr mutation
(0 available);
any
Gsc mutation
(15 available)
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mortality/aging
embryo
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• pyknotic cells are seen in the mesenchyme of the first branchial arches
• the first branchial arch arteries are either not visible or poorly formed
• epithelium of the first arch fails to fuse ventrally with the pharyngeal endoderm
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• the first branchial arch arteries are either not visible or poorly formed
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• at E9.5
• expression analysis indicates reduced populations of both mesodermal and neural crest cells
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• pyknotic cells are seen in the mesenchyme of the second branchial arches
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• at E9.5
• expression analysis indicates reduced populations of both mesodermal and neural crest cells
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• at E8.75 defects are seen in the anterior end of the embryo including the region of the heart; however, development posterior to the heart is normal
• variable severity of the defects at E9.0 - E9.5
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• at E9.0 - E9.5 dorsal-ventral patterning of the forebrain is abnormal
• at E9.0 - E9.5 in severely affected embryos expression analysis indicates ventral expansion of dorsal cell fates in floor plate cells with resulting loss of ventral structures
• however, anterior-posterior patterning of the neural tube is unaffected
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• arrest around the 20 - 25 somite stage
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• at E9.5 in severely affected embryos the floor and roof of the neural tube are in contact with each other at the diencephalic-mesencephalic junction
• at E9.0 - E9.5 in severely affected embryos expression analysis indicates ventral expansion of dorsal cell fates in floor plate cells with resulting loss of ventral structures
• however, anterior-posterior patterning of the neural tube is unaffected
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• at E9.5 in less severely affected embryos thinning of the neuroepithelium is seen in the forebrain, hindbrain, and spinal cord
• reduction in neuroepithelium is most severe in the forebrain
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• in severely affected embryos notochord cells disappear from the rostral half of the embryo by E9.5
• however, in less severely affected embryos notochord cells are maintained
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• reduced in size at E9.0 - E9.5
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cardiovascular system
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• at E9.5 the dorsal aorta is elongated, enlarged, or disorganized
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• the first branchial arch arteries are either not visible or poorly formed
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• greatly enlarged anterior cardinal veins at E9.5
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• by E9.5, the dorsal mesocardium fails to form resulting in connection of the heart to the gut
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• defects in heart looping are seen at E8.75
• at E9.5 the heart is a straight or S-shaped tube at the midline
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• the dorsal mesocardium fails to form
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vision/eye
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• smaller at E9.5 in less severely affected embryos
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• at E9.5 in more severely affected embryos the optic vesicle is lost as a result of lack of maintenance as optic vesicles are present at E8.75
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nervous system
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• at E9.5 in severely affected embryos the floor and roof of the neural tube are in contact with each other at the diencephalic-mesencephalic junction
• at E9.0 - E9.5 in severely affected embryos expression analysis indicates ventral expansion of dorsal cell fates in floor plate cells with resulting loss of ventral structures
• however, anterior-posterior patterning of the neural tube is unaffected
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• at E9.5 in less severely affected embryos thinning of the neuroepithelium is seen in the forebrain, hindbrain, and spinal cord
• reduction in neuroepithelium is most severe in the forebrain
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• dorsal-ventral patterning is abnormal
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• dramatically reduced at E8.75
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• reduced in size at E9.0 - E9.5
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respiratory system
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• smaller and abnormally shaped
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growth/size/body
craniofacial
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• pyknotic cells are seen in the mesenchyme of the first branchial arches
• the first branchial arch arteries are either not visible or poorly formed
• epithelium of the first arch fails to fuse ventrally with the pharyngeal endoderm
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• the first branchial arch arteries are either not visible or poorly formed
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• at E9.5
• expression analysis indicates reduced populations of both mesodermal and neural crest cells
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• pyknotic cells are seen in the mesenchyme of the second branchial arches
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• at E9.5
• expression analysis indicates reduced populations of both mesodermal and neural crest cells
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Allelic
Composition |
Foxa2tm1Jrt/Foxa2+
Nodaltm1Rob/Nodal+
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Genetic
Background |
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ |
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embryo
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• in embryos with loss of left-right asymmetry in Nodal expression the direction of turning is randomized
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growth/size/body
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• in 7 of 10 mutants the positioning of the abdominal viscera and heart is abnormal
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxa2tm1Jrt mutation
(2 available);
any
Foxa2 mutation
(26 available)
Gsctm1Bhr mutation
(0 available);
any
Gsc mutation
(15 available)
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embryo
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• phenotype at E9.5 is similar to that of Foxa2 single homozygotes with no increase in the severity of the defect in development of the anterior posterior axis
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• embryos are small and thin
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growth/size/body
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• embryos are small and thin
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxa2tm1Jrt mutation
(2 available);
any
Foxa2 mutation
(26 available)
Gsctm1Bhr mutation
(0 available);
any
Gsc mutation
(15 available)
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embryo
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• phenotype at E9.5 is similar to that of Foxa2 single homozygotes with no increase in the severity of the defect in development of the anterior posterior axis
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• embryos are small and thin
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growth/size/body
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• embryos are small and thin
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Analysis Tools