Phenotypes associated with this allele

• Allele Symbol: Cripto^tm1Eda
• Allele Name: targeted mutation 1, Eileen D Adamson
• Allele ID: MGI:1857518
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cripto^tm1Eda/Cripto^tm1Eda	either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * Black Swiss * C57BL/6)	MGI:5433710
hm2	Cripto^tm1Eda/Cripto^tm1Eda	either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * Black Swiss) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)	MGI:3851255
cx3	Cer1^tm1Belo/Cer1^tm1Belo Cripto^tm1Eda/Cripto^tm1Eda	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3851256

Genotype
MGI:5433710

hm1

• Allelic Composition: Cripto^tm1Eda/Cripto^tm1Eda
• Genetic Background: either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * Black Swiss * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Gastrulation defects in Cripto^tm1.1Gmin and Cripto^tm1Eda homozygous embryos

embryo

abnormal gastrulation ( J:186907 )

• indicated by expression analysis

abnormal mesoderm development ( J:186907 )

• absence of posterior mesoderm cell populations

embryonic growth arrest ( J:186907 )

abnormal primitive streak formation ( J:186907 )

• indicated by expression analysis

Genotype
MGI:3851255

hm2

• Allelic Composition: Cripto^tm1Eda/Cripto^tm1Eda
• Genetic Background: either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * Black Swiss) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)

mortality/aging

embryonic lethality, complete penetrance ( J:51595 )

• no homozygous progeny are produced

• normal at E6.5 but abnormal at E7.5

• no phenotype described for mice older than E10.5

embryo

embryo phenotype ( J:51595 )

N • all three germ layers present at E7.5

N • extra-embryonic visceral and parietal endoderm present

abnormal developmental patterning ( J:51595 )

• no headfolds at E8.5

abnormal mesoderm development ( J:51595 )

• abnormally compacted at E8.5

• lacks normal matrix materials

abnormal rostral-caudal axis patterning ( J:51595 )

• embryos abnormally inflected

short rostral-caudal axis ( J:51595 )

• shortened axis

abnormal embryonic tissue morphology ( J:51595 )

• embryonic region is distorted and small at R7.5

• clear embryonic defects at E8.5

abnormal embryonic neuroepithelium morphology ( J:51595 )

• distorted as a result of abnormally inflected embryo

• grossly abnormal at E8.5

absent somites ( J:51595 )

• no somites at E8.5

abnormal extraembryonic tissue morphology ( J:51595 )

• embryos are composed primarily of extra-embryonic tissues at E9.5

• yolk sac and placenta present

• trophoblast giant cells present at E7.5

absent vitelline blood vessels ( J:51595 )

• blood vessels fail to form in the visceral yolk sac by E10.5

abnormal amnion morphology ( J:51595 )

• somewhat abnormal at E7.5

abnormal chorion morphology ( J:51595 )

• somewhat abnormal at E7.5

abnormal visceral yolk sac blood island morphology ( J:51595 )

• blood islands are small at E9.5

nervous system

abnormal embryonic neuroepithelium morphology ( J:51595 )

• distorted as a result of abnormally inflected embryo

• grossly abnormal at E8.5

cardiovascular system

absent vitelline blood vessels ( J:51595 )

• blood vessels fail to form in the visceral yolk sac by E10.5

abnormal heart development ( J:51595 )

• no beating cardiomyocytes ever observed

cellular

abnormal cell adhesion ( J:51595 )

• reduced ability of isolated embryonic fibroblasts to attach to a matrix

decreased fibroblast proliferation ( J:51595 )

• growth rate of isolated embryonic fibroblasts is about 50% that of controls

Genotype
MGI:3851256

cx3

• Allelic Composition: Cer1^tm1Belo/Cer1^tm1Belo; Cripto^tm1Eda/Cripto^tm1Eda
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

embryo

embryo phenotype ( J:132584 )

N • well developed embyonic region at E9.5

N • migration of anterior visceral endoderm is rescued

abnormal rostral-caudal axis patterning ( J:132584 )

• anterior-posterior axis has developed by E6.5

• delayed at E9.5 relative to controls (equivalent to E8)

• well developed anterior-posterior axis at E8

nervous system

nervous system phenotype ( J:132584 )

N • posterior neuroectoderm is present at E12.5

cardiovascular system

cardiovascular system phenotype ( J:132584 )

N • developing heart is present at E12.5