Analysis Tools
mortality/aging
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• homozygotes are obtained at the expected Mendelian frequency at E8.5 but die by E10.5
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embryo
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• at E9.5, homozygotes show absence of embryonic (nucleated) blood cells within endothelial-lined capillaries in the fetal labyrinth
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• at E9.5-E10, homozygotes display extensive necrosis
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• at E9.5-E10, homozygotes exhibit a delay in overall growth
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• at E9.5-E10, homozygotes are easily distinguished by their smaller size
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• at E9.5, homozygotes exhibit an average of 21 somite pairs vs >25 pairs found in wild-type embryos
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• at E9.5-E10, mutant yolk sacs show absence of nucleated red blood cells
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• at E9.5-E10, mutant embryos display total absence of embryonic (nucleated) red cells
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hematopoietic system
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• at E9.5-E10, mutant embryos display total absence of embryonic (nucleated) red cells
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• in vitro, yolk sac cells obtained from E8.5-E9.0 homozygotes show a virtual absence of myeloid colony formation, suggesting a deficit at the myelo-erythroid progenitor level
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cardiovascular system
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• at E9.5, homozygotes show absence of embryonic (nucleated) blood cells within endothelial-lined capillaries in the fetal labyrinth
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• at E9.5-E10, homozygotes display dilated pericardial sacs
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growth/size/body
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• at E9.5-E10, homozygotes exhibit a delay in overall growth
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• at E9.5-E10, homozygotes are easily distinguished by their smaller size
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integument
cellular
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• at E9.5-E10, homozygotes display extensive necrosis
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