All Phenotypes Lck<tm1Mak> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lck^tm1Mak/Lck^tm1Mak	B6.129S2-Lck^tm1Mak	MGI:4834472
hm2	Lck^tm1Mak/Lck^tm1Mak	involves: 129S2/SvPas	MGI:2181732
cx3	Il2rb^tm1Mak/Il2rb^tm1Mak Lck^tm1Mak/Lck^tm1Mak	involves: 129	MGI:3798942
cx4	Fyn^tm1Rmp/Fyn^tm1Rmp Lck^tm1Mak/Lck^tm1Mak	involves: 129S2/SvPas	MGI:4360346
cx5	Lck^tm1Mak/Lck^tm1Mak Rag1^tm1Mom/Rag1⁺ Tg(Lck-Fyn*Y528F)5525Cjg/0	involves: 129S2/SvPas * 129S7/SvEvBrd * C57BL/6	MGI:4360345
cx6	Cd247^tm1Mal/Cd247^tm1Mal Lck^tm1Mak/Lck^tm1Mak	involves: 129S2/SvPas * C57BL/6	MGI:4357681

Allelic Composition	Lck^tm1Mak/Lck^tm1Mak
Genetic Background	B6.129S2-Lck^tm1Mak

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lck^tm1Mak mutation (1 available); any Lck mutation (93 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal T cell differentiation ( J:111646 )

• intestinal intraepithelial lymphocytes (iIELs) is impaired

• however, extrathymic development of iIELs is normal in reconstitution experiments

abnormal intraepithelial T cell morphology ( J:111646 )

• in reconstitution experiments, intrathymic development of intestinal intraepithelial lymphocytes (iIELs) is impaired

• however, extrathymic development of iIELs is normal in reconstitution experiments

abnormal intraepithelial T cell number ( J:111646 )

• at 10 weeks, the number of intestinal intraepithelial lymphocytes (iIELs) is increased 1.5- to 2-fold compared to in wild-type mice

• the number of iIELs decrease over time unlike in wild-type mice that exhibit an increase in these cells

decreased gamma-delta intraepithelial T cell number ( J:111646 )

• at 10 weeks, mice exhibit decreased gamma-delta intestinal intraepithelial lymphocytes, which continue to decrease in number with age, compared with wild-type mice

decreased cytotoxic T cell cytolysis ( J:111646 )

• intestinal intraepithelial lymphocytes exhibit a slight reduction in cytolytic activities compared with wild-type cells

hematopoietic system

abnormal T cell differentiation ( J:111646 )

• intestinal intraepithelial lymphocytes (iIELs) is impaired

• however, extrathymic development of iIELs is normal in reconstitution experiments

abnormal intraepithelial T cell morphology ( J:111646 )

• in reconstitution experiments, intrathymic development of intestinal intraepithelial lymphocytes (iIELs) is impaired

• however, extrathymic development of iIELs is normal in reconstitution experiments

abnormal intraepithelial T cell number ( J:111646 )

• at 10 weeks, the number of intestinal intraepithelial lymphocytes (iIELs) is increased 1.5- to 2-fold compared to in wild-type mice

• the number of iIELs decrease over time unlike in wild-type mice that exhibit an increase in these cells

decreased gamma-delta intraepithelial T cell number ( J:111646 )

• at 10 weeks, mice exhibit decreased gamma-delta intestinal intraepithelial lymphocytes, which continue to decrease in number with age, compared with wild-type mice

decreased cytotoxic T cell cytolysis ( J:111646 )

• intestinal intraepithelial lymphocytes exhibit a slight reduction in cytolytic activities compared with wild-type cells

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

thymus atrophy ( J:1119 )

• the thymus is sparsely populated with lymphoid cells and has a very thin medulla

thymus hypoplasia ( J:37062 , J:37940 )

• thymus cellularity is 10-20% of normal (J:37062)

• thymic cellularity is 10-fold less than controls (J:37940)

abnormal thymocyte activation ( J:110847 )

• double positive thymocytes do not efficiently enter into the cell cycle

abnormal lymphocyte morphology ( J:1119 )

• although the overall numbers of cells in the lymph nodes and spleens are normal the B/T cells ratio is inverted with 90 to 95% B cells and 5 to 10% T cells

abnormal T cell morphology ( J:110847 )

• double-positive thymocytes have a slightly lower expression of alpha-beta TCR

abnormal double-negative T cell morphology ( J:110847 )

• there is a partial block in development in the DN3 stage that leads to a higher percentage of these thymocytes

decreased T cell number ( J:1119 , J:37062 )

• the number of peripheral T cells is reduced (J:1119)

• alpha-beta T cell numbers are reduced about 10-20 fold compared to controls (J:37062)

decreased thymocyte number ( J:1119 )

• 6x10⁶ to 2x10⁷ cells compared to 2x10⁸ cells in a wild-type thymus

decreased double-positive T cell number ( J:1119 , J:110847 )

• 0.3x10⁷ to 1.6x10⁷ compared to 1.8x10⁸ cells in wild-type mice (J:1119)

• only 12% of thymocytes are double-positive compared to 97% in controls (J:110847)

decreased CD4-positive, alpha-beta T cell number ( J:37940 )

• CD4 T cell numbers are reduced in the periphery

decreased CD8-positive, alpha-beta T cell number ( J:37940 )

• CD8 T cell numbers are reduced in the periphery

decreased gamma-delta T cell number ( J:37062 )

• gamma-delta T cell numbers are reduced about 2-fold compared to controls

decreased gamma-delta intraepithelial T cell number ( J:37940 , J:112977 )

• dendritic epithelial T cell numbers are reduced in the skin (J:37940)

• gamma-delta IEL are absent in these mice (J:112977)

• a CD3^- IEL population believed to represent a precursor population is present in small intestine epithelium (J:112977)

abnormal alpha-beta intraepithelial T cell morphology ( J:112977 )

• alpha-beta IEL are absent in these mice

• a CD3^- IEL population believed to represent a precursor population is present in small intestine epithelium

abnormal single-positive T cell number ( J:37062 )

• very few single-positive thymocytes are detected in these mice and even fewer express mature levels of TCR, CD5 and CD69

decreased IgG1 level ( J:1119 )

increased IgG2a level ( J:1119 )

• marginal

increased IgG3 level ( J:1119 )

• marginal

increased IgM level ( J:1119 )

hematopoietic system

thymus atrophy ( J:1119 )

• the thymus is sparsely populated with lymphoid cells and has a very thin medulla

thymus hypoplasia ( J:37062 , J:37940 )

• thymus cellularity is 10-20% of normal (J:37062)

• thymic cellularity is 10-fold less than controls (J:37940)

abnormal thymocyte activation ( J:110847 )

• double positive thymocytes do not efficiently enter into the cell cycle

abnormal lymphocyte morphology ( J:1119 )

• although the overall numbers of cells in the lymph nodes and spleens are normal the B/T cells ratio is inverted with 90 to 95% B cells and 5 to 10% T cells

abnormal T cell morphology ( J:110847 )

• double-positive thymocytes have a slightly lower expression of alpha-beta TCR

abnormal double-negative T cell morphology ( J:110847 )

• there is a partial block in development in the DN3 stage that leads to a higher percentage of these thymocytes

decreased T cell number ( J:1119 , J:37062 )

• the number of peripheral T cells is reduced (J:1119)

• alpha-beta T cell numbers are reduced about 10-20 fold compared to controls (J:37062)

decreased thymocyte number ( J:1119 )

• 6x10⁶ to 2x10⁷ cells compared to 2x10⁸ cells in a wild-type thymus

decreased double-positive T cell number ( J:1119 , J:110847 )

• 0.3x10⁷ to 1.6x10⁷ compared to 1.8x10⁸ cells in wild-type mice (J:1119)

• only 12% of thymocytes are double-positive compared to 97% in controls (J:110847)

decreased CD4-positive, alpha-beta T cell number ( J:37940 )

• CD4 T cell numbers are reduced in the periphery

decreased CD8-positive, alpha-beta T cell number ( J:37940 )

• CD8 T cell numbers are reduced in the periphery

decreased gamma-delta T cell number ( J:37062 )

• gamma-delta T cell numbers are reduced about 2-fold compared to controls

decreased gamma-delta intraepithelial T cell number ( J:37940 , J:112977 )

• dendritic epithelial T cell numbers are reduced in the skin (J:37940)

• gamma-delta IEL are absent in these mice (J:112977)

• a CD3^- IEL population believed to represent a precursor population is present in small intestine epithelium (J:112977)

abnormal alpha-beta intraepithelial T cell morphology ( J:112977 )

• alpha-beta IEL are absent in these mice

• a CD3^- IEL population believed to represent a precursor population is present in small intestine epithelium

abnormal single-positive T cell number ( J:37062 )

• very few single-positive thymocytes are detected in these mice and even fewer express mature levels of TCR, CD5 and CD69

decreased IgG1 level ( J:1119 )

increased IgG2a level ( J:1119 )

• marginal

increased IgG3 level ( J:1119 )

• marginal

increased IgM level ( J:1119 )

endocrine/exocrine glands

thymus atrophy ( J:1119 )

• the thymus is sparsely populated with lymphoid cells and has a very thin medulla

thymus hypoplasia ( J:37062 , J:37940 )

• thymus cellularity is 10-20% of normal (J:37062)

• thymic cellularity is 10-fold less than controls (J:37940)

decreased thymocyte number ( J:1119 )

• 6x10⁶ to 2x10⁷ cells compared to 2x10⁸ cells in a wild-type thymus

abnormal thymocyte activation ( J:110847 )

• double positive thymocytes do not efficiently enter into the cell cycle

Allelic Composition	Il2rb^tm1Mak/Il2rb^tm1Mak Lck^tm1Mak/Lck^tm1Mak
Genetic Background	involves: 129

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il2rb^tm1Mak mutation (5 available); any Il2rb mutation (45 available)
Lck^tm1Mak mutation (1 available); any Lck mutation (93 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

abnormal maternal decidual layer morphology ( J:134589 )

• the decidual region is deficient in normal decidual cells and appears as a large area of adipose tissue

absent uterine NK cells ( J:134589 )

• uterine NK cells are virtually absent in the uteri of pregnant mice at day 14 of gestation

abnormal endometrium morphology ( J:134589 )

• the metrial gland and uterine NK cells are absent from the uteri of pregnant mice at day 14 of gestation

absent endometrial glands ( J:134589 )

• the metrial gland is absent from the uteri of pregnant mice at day 14 of gestation

immune system

absent uterine NK cells ( J:134589 )

• uterine NK cells are virtually absent in the uteri of pregnant mice at day 14 of gestation

embryo

abnormal maternal decidual layer morphology ( J:134589 )

• the decidual region is deficient in normal decidual cells and appears as a large area of adipose tissue

absent uterine NK cells ( J:134589 )

• uterine NK cells are virtually absent in the uteri of pregnant mice at day 14 of gestation

hematopoietic system

absent uterine NK cells ( J:134589 )

• uterine NK cells are virtually absent in the uteri of pregnant mice at day 14 of gestation

endocrine/exocrine glands

absent uterine NK cells ( J:134589 )

• uterine NK cells are virtually absent in the uteri of pregnant mice at day 14 of gestation

absent endometrial glands ( J:134589 )

• the metrial gland is absent from the uteri of pregnant mice at day 14 of gestation

Allelic Composition	Fyn^tm1Rmp/Fyn^tm1Rmp Lck^tm1Mak/Lck^tm1Mak
Genetic Background	involves: 129S2/SvPas

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fyn^tm1Rmp mutation (1 available); any Fyn mutation (37 available)
Lck^tm1Mak mutation (1 available); any Lck mutation (93 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

thymus hypoplasia ( J:37062 , J:37940 )

• thymus cellularity is 1-5% of normal (J:37062)

• thymic cellularity is 50- to 100-fold less than controls (J:37940)

decreased double-positive T cell number ( J:37940 )

• double-positive thymocytes are virtually absent

arrested T cell differentiation ( J:37062 , J:37940 )

• T cell development is blocked in the transition from double-negative to double-positive stage (J:37062)

• the thymocytes are predominately CD25⁺ and HAS^hi (J:37062)

• T cell development is arrested at the CD44^-CD25⁺ stage with 85-92% of thymocytes being at this stage (J:37940)

absent CD4-positive, alpha-beta T cells ( J:37940 )

• CD4 T cells are virtually absent from the periphery

absent CD8-positive, alpha-beta T cells ( J:37940 )

• CD8 T cells are virtually absent from the periphery

abnormal NK T cell number ( J:37940 )

• NK1.1⁺ alpha-beta T cells are virtually absent from the periphery

decreased gamma-delta intraepithelial T cell number ( J:37940 , J:112977 )

• dendritic epithelial T cell numbers are severely reduced in the skin (J:37940)

• CD8alpha-alpha gamma-delta IEL are present but in smaller numbers (J:112977)

absent T cells ( J:37062 )

• alpha-beta and gamma-delta T cells are absent in these mice

abnormal alpha-beta intraepithelial T cell morphology ( J:112977 )

• alpha-beta IEL are absent in these mice

• gamma-delta IEL are still present

hematopoietic system

thymus hypoplasia ( J:37062 , J:37940 )

• thymus cellularity is 1-5% of normal (J:37062)

• thymic cellularity is 50- to 100-fold less than controls (J:37940)

decreased double-positive T cell number ( J:37940 )

• double-positive thymocytes are virtually absent

arrested T cell differentiation ( J:37062 , J:37940 )

• T cell development is blocked in the transition from double-negative to double-positive stage (J:37062)

• the thymocytes are predominately CD25⁺ and HAS^hi (J:37062)

• T cell development is arrested at the CD44^-CD25⁺ stage with 85-92% of thymocytes being at this stage (J:37940)

absent CD4-positive, alpha-beta T cells ( J:37940 )

• CD4 T cells are virtually absent from the periphery

absent CD8-positive, alpha-beta T cells ( J:37940 )

• CD8 T cells are virtually absent from the periphery

abnormal NK T cell number ( J:37940 )

• NK1.1⁺ alpha-beta T cells are virtually absent from the periphery

decreased gamma-delta intraepithelial T cell number ( J:37940 , J:112977 )

• dendritic epithelial T cell numbers are severely reduced in the skin (J:37940)

• CD8alpha-alpha gamma-delta IEL are present but in smaller numbers (J:112977)

absent T cells ( J:37062 )

• alpha-beta and gamma-delta T cells are absent in these mice

abnormal alpha-beta intraepithelial T cell morphology ( J:112977 )

• alpha-beta IEL are absent in these mice

• gamma-delta IEL are still present

endocrine/exocrine glands

thymus hypoplasia ( J:37062 , J:37940 )

• thymus cellularity is 1-5% of normal (J:37062)

• thymic cellularity is 50- to 100-fold less than controls (J:37940)

Allelic Composition	Lck^tm1Mak/Lck^tm1Mak Rag1^tm1Mom/Rag1⁺ Tg(Lck-Fyn*Y528F)5525Cjg/0
Genetic Background	involves: 129S2/SvPas * 129S7/SvEvBrd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lck^tm1Mak mutation (1 available); any Lck mutation (93 available)
Rag1^tm1Mom mutation (49 available); any Rag1 mutation (120 available)
Tg(Lck-Fyn*Y528F)5525Cjg mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal T cell differentiation ( J:37062 )

• the transgene partially rescues arrest of T cell development associated with Lck deficiency

• thymocyte numbers are normal

• splenic gamma-delta T cell numbers are normal

• expression of TCR and CD5 on transitional single-positive thymocytes is slightly lower than controls

decreased T cell number ( J:37062 )

• alpha beta T cell numbers are less than half of wild-type controls

immune system

abnormal T cell differentiation ( J:37062 )

• the transgene partially rescues arrest of T cell development associated with Lck deficiency

• thymocyte numbers are normal

• splenic gamma-delta T cell numbers are normal

• expression of TCR and CD5 on transitional single-positive thymocytes is slightly lower than controls

decreased T cell number ( J:37062 )

• alpha beta T cell numbers are less than half of wild-type controls

Allelic Composition	Cd247^tm1Mal/Cd247^tm1Mal Lck^tm1Mak/Lck^tm1Mak
Genetic Background	involves: 129S2/SvPas * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd247^tm1Mal mutation (1 available); any Cd247 mutation (11 available)
Lck^tm1Mak mutation (1 available); any Lck mutation (93 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal thymocyte activation ( J:110847 )

• minimal numbers of double positive thymocytes enter into the cell cycle during the double positive stage

abnormal T cell morphology ( J:110847 )

• thymocytes fail to express an alphabeta TCR

abnormal T cell differentiation ( J:110847 )

• there is a partial block in development in the DN3 stage that is more severe in the double homozygote null mice than in the singly- homozygote null mice

abnormal double-negative T cell morphology ( J:110847 )

• there is a partial block in development in the DN3 stage that leads to a higher percentage of these thymocytes

decreased double-positive T cell number ( J:110847 )

• the double-positive thymocyte population is slightly larger by nine weeks of age

• double-positive thymocytes are almost absent from the thymus in 6 week old mice

hematopoietic system

abnormal thymocyte activation ( J:110847 )

• minimal numbers of double positive thymocytes enter into the cell cycle during the double positive stage

abnormal T cell morphology ( J:110847 )

• thymocytes fail to express an alphabeta TCR

abnormal T cell differentiation ( J:110847 )

• there is a partial block in development in the DN3 stage that is more severe in the double homozygote null mice than in the singly- homozygote null mice

abnormal double-negative T cell morphology ( J:110847 )

• there is a partial block in development in the DN3 stage that leads to a higher percentage of these thymocytes

decreased double-positive T cell number ( J:110847 )

• double-positive thymocytes are almost absent from the thymus in 6 week old mice

• the double-positive thymocyte population is slightly larger by nine weeks of age

endocrine/exocrine glands

abnormal thymocyte activation ( J:110847 )

• minimal numbers of double positive thymocytes enter into the cell cycle during the double positive stage

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