Gna14^em1Dlee
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gna14^em1Dlee
• Name: guanine nucleotide binding protein, alpha 14; endonuclease-mediated mutation 1, Daekee Lee
• MGI ID: MGI:8449338
• Gene: Gna14 Location: Chr19:16413126-16588184 bp, + strand Genetic Position: Chr19, 11.29 cM
• Alliance: Gna14^em1Dlee page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details:

  CRISPR/Cas9-mediated recombination using sgRNAs 5-ACGTGGTATTTCCTTTCCGT-3 and 5-CCAAATAGTGAGTACTCAGC-3 located in introns 2 and 3 generated a 175 bp deletion that includes the entire exon 3. Western blot analysis confirmed the absence of protein in kidney. (J:341358)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gna14 Mutation: 22 strains or lines available

References

• Original: J:341358 Park R, et al., Tumor-Promoting Role of GNA14 in Colon Cancer Development. Cancers (Basel). 2023 Sep 15;15(18)
• All: 1 reference(s)