Gt(ROSA)26Sor^{em4.1(CAG-MTRFR*)Rwb}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em4.1(CAG-MTRFR*)Rwb}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 4.1, Robert W Burgess
• MGI ID: MGI:8433685
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em4.1(CAG-MTRFR*)Rwb} page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Insertion
• Mutation details:

  Cre-mediated excision of the lox-stop-lox in the em4(CAG-MTRFR*)Rwb allele resulted in this allele in which a CAG promoter driving expression of the sequence for human MTRFR with the sequence modified to replace the lysines at residues 146, 153, and 156 with alanines. (J:370627)

• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1212 strains or lines available

References

• Original: J:370627 Pratt SL, et al., Evaluating the feasibility of gene replacement strategies to treat MTRFR deficiency. Dis Model Mech. 2025 May 1;18(5)
• All: 1 reference(s)